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The map label for this gene is tag [H]

Identifier: 83311673

GI number: 83311673

Start: 2759273

End: 2759845

Strand: Reverse

Name: tag [H]

Synonym: amb2574

Alternate gene names: 83311673

Gene position: 2759845-2759273 (Counterclockwise)

Preceding gene: 83311674

Following gene: 83311672

Centisome position: 55.56

GC content: 64.75

Gene sequence:

>573_bases
GTGGCCGAGGAGAAACAAATGTCTTGGTATTGCGACGTCGCGCCCGGCCATCCGTTCCACGGTCCCTATCATGACCGGGA
ATACGGCTTTCCCGTTGCCGACGAAGCGGTGCTGTTCGAGCGCCTTTGCCTGGAGATCTTTCAGGCGGGGTTATCCTGGC
TGATCGTGCTGAAGAAGCGCCCGGCCATGGCGGCGGCCTTCGACGGTTTCGAGGTGGACAAGGTGGCCGCCTATGGCGAG
GCCGAGCTGGAACGGCTGATGAATGATTCCGGCATCATCCGCAACCGCCGCAAGCTGGCCGCCATCGTCGAGAATGCCCG
TCGGCTGCAGGCCCTGCGGGCGCGCGAGGGCAGTTTCGCCGCCTGGCTGGCGCGGCACCATCCCCTGACCAAGGATGGCT
GGGTCAAGCTGTTCAAGGCCACGTTCGTCTTCGTGGGCGGTGAGGTGGTGGGGGAGTTTCTGATGAGCATCGGCTACCTG
CCGGGAGGCCATCGCGAGGATTGCCCGGTCCAGGCGCGGCTTCGCGCTGCCGGCGCACCCTGCTTCGCGGTCGATCCCGC
TTTTTATGCTTGA

Upstream 100 bases:

>100_bases
TCCTGCACCCCAAGGATTTCAACGGCACCCTGGTCGAGCTGGAACAGGCTTAGCCGGACTCCAACCCTCCTCGGCCACGC
GGGGGTGGCGGGGCGGCCGC

Downstream 100 bases:

>100_bases
GGTGGGGGCCTGAAATGGGAAACGACAGGACCGCCTTGGCGATCCTGCCGCATCCTACGTTCCTGGCCTCGGCCTTGACG
GCGGACCTGGGACACGTTCC

Product: 3-methyladenine DNA glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAEEKQMSWYCDVAPGHPFHGPYHDREYGFPVADEAVLFERLCLEIFQAGLSWLIVLKKRPAMAAAFDGFEVDKVAAYGE
AELERLMNDSGIIRNRRKLAAIVENARRLQALRAREGSFAAWLARHHPLTKDGWVKLFKATFVFVGGEVVGEFLMSIGYL
PGGHREDCPVQARLRAAGAPCFAVDPAFYA

Sequences:

>Translated_190_residues
MAEEKQMSWYCDVAPGHPFHGPYHDREYGFPVADEAVLFERLCLEIFQAGLSWLIVLKKRPAMAAAFDGFEVDKVAAYGE
AELERLMNDSGIIRNRRKLAAIVENARRLQALRAREGSFAAWLARHHPLTKDGWVKLFKATFVFVGGEVVGEFLMSIGYL
PGGHREDCPVQARLRAAGAPCFAVDPAFYA
>Mature_189_residues
AEEKQMSWYCDVAPGHPFHGPYHDREYGFPVADEAVLFERLCLEIFQAGLSWLIVLKKRPAMAAAFDGFEVDKVAAYGEA
ELERLMNDSGIIRNRRKLAAIVENARRLQALRAREGSFAAWLARHHPLTKDGWVKLFKATFVFVGGEVVGEFLMSIGYLP
GGHREDCPVQARLRAAGAPCFAVDPAFYA

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=169, Percent_Identity=35.5029585798817, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21245; Mature: 21113

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKQMSWYCDVAPGHPFHGPYHDREYGFPVADEAVLFERLCLEIFQAGLSWLIVLKKR
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEHCCCC
PAMAAAFDGFEVDKVAAYGEAELERLMNDSGIIRNRRKLAAIVENARRLQALRAREGSFA
CCHHHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AWLARHHPLTKDGWVKLFKATFVFVGGEVVGEFLMSIGYLPGGHREDCPVQARLRAAGAP
HHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
CFAVDPAFYA
EEECCCCCCC
>Mature Secondary Structure 
AEEKQMSWYCDVAPGHPFHGPYHDREYGFPVADEAVLFERLCLEIFQAGLSWLIVLKKR
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEHCCCC
PAMAAAFDGFEVDKVAAYGEAELERLMNDSGIIRNRRKLAAIVENARRLQALRAREGSFA
CCHHHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AWLARHHPLTKDGWVKLFKATFVFVGGEVVGEFLMSIGYLPGGHREDCPVQARLRAAGAP
HHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
CFAVDPAFYA
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]