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The map label for this gene is slt [H]

Identifier: 83311638

GI number: 83311638

Start: 2721576

End: 2723588

Strand: Reverse

Name: slt [H]

Synonym: amb2539

Alternate gene names: 83311638

Gene position: 2723588-2721576 (Counterclockwise)

Preceding gene: 83311639

Following gene: 83311637

Centisome position: 54.83

GC content: 68.7

Gene sequence:

>2013_bases
ATGCCTGCCCTGTTGAACAATCGGCCAGGATTCCAGCCGTTGAAGCTTTTCCCCGTCGCCATCATCCTTTCCGCTCTTCT
TTGCCTGGGGGCACCGTCTGCCGCCCGGGCTGACGGGGTCGGGGATGCCGCCATCGCCCGCCAGGCCATCGCCGCCGCCA
AGCGCGATCATTTCGACGAGGCCGAGCGGCTGGCGCGGCAATCGCGCTCAAAGGCGCTGCCCCGCCTGGTGACCTGGATG
GCCTACGTCTCCGGCCGGTCGGGCGCCGATTTCGCCCAGCTCGGCGCCTTCATCCACGCCAATCCCGAATGGCCGATGAT
GAGCCAGATGACCAAGCGGGCCGAGGAATCCATTACCGCCGCCACGCCCACGGCCCAGGTTCTGGCCTGGTTCGACTCGC
ACCCGCCCACCACCGCCGATGGCGGTCAGGCCTATGCGCGCGCGCTGTTCGCCGCCGGGCGGAACGAGCAGGCGGTCAAG
GTCATCCGCGAGACCTGGGTCAATCTGAGCTTCGGGGCGCTGCAGGAAAAGCAGTACCTCAACCTCCTGGGCGAGCATCT
GCGCTACGAGGACCACTGGCGCCGCCTGGACCGGCTGCTGTGGGACCGGCAGGAGACCTCGGTCCAGCGCATGATCATGA
AGGTGGATGCCGGCCATCGCGCCGTGGCCCAGGCCCGCCTCGCCCTGCAGGCGGGCAAGTCCAATCCCGAGCCGCTGATC
AATGCCGTTCCCGCCAGCTTGCGTGACGATCCCGGCCTGATCTACGAGCGGGTGCGCTGGCGCCGCCAGAAGGACCTGGA
CGAGGATGCGCTCGACCTGCTGTCCCACCCGTCGCGCAACAAGGTCCGCCCGGATCTGTGGTGGCAGGAGCGGGCCATCC
TGGCCCGGCGCGCCCTGCAGAAGGGGCTGGTCTCGAGGGCCTATCAGGCCGCCGCCGATCACGGGCTGGAAGGGGGCACC
CAATATGTGGACGCCGAGTTCCTGGCCGGCTGGGTCGCGCTGCGCTTCCTGGATGACCGCGCCACTGCCGTCCACCACTT
CACCCGTCTGCATGAATGGGCCAGTCACCCCATTTCGCGGGCTCGTGCCGCCTATTGGGCCGGGCGTGCCCTGGAGGCGG
CGGGGGATGCCAAGGCCAAGGAGTGGTATACCCGGGCGGCCCGCTATTCCACCACCTATTACGGCCAGCTGGGCGCGTCG
CGCCTGGGCGATCATCATTGGCCGCTGCCCGACGAGCCGCAGCCGACCCCCGACGACGTGGCCCGCTTCGAGGCCCGCGA
CGTGGTGGCCGCCGCCCGCCTGCTGATGCAGGTGGGGGAGAGCGAATTGCTGCGCTCATTCTTCATCCGCCTCAACGACA
CCGTCCAGACTCCGGGGGAACGCGCCCTGGTGGCCGGACTGGCCAGCCGGACCGGGCGGCACGACCTGGGCCTGACCGTG
GCGCGCCGCTCCGACCGCGAGGGGGTGACCCTGGTCCAGGCCGGCTGGCCGGTGCCTGACCTGGACGCCGACGAGACCAA
TCCGGAAAAGGCCCTGGTTCTCGCCCTGATTCGCCAGGAGAGCGGCTTCGTCGCCGATATCGAATCGCCGGCCGGGGCCA
AGGGCCTGATGCAGTTGCTGCCGTCCACCGCGTCCAAGGTGGCCAAGAGCATCGGACTCAAATACCACGTCAACAAGCTG
GATGATCCCAACTTCAACGTTCAGGTGGGCTCGGCCTATCTGCGCGATCTGGTGGGGGATTTCGAGGGCTCCTACATCCT
GGCCCTGGCGTCCTACAATGCCGGGCCGGGGCGGGCGCGGCGCTGGATTCGCGAATACGGCGATCCCCGCGACGCCAATG
TGGACGTGGTCGACTGGGTGGAAATGATTCCCTTCAGCGAGACCCGCAATTACGTGCAGCGGGTGATGGAAAGCGTCGCC
GTCTACCGCCGCCGCCTGGGCAAGCATGTGGGGCCGACCCTGGAGGCCGATCTGAAGCGCTGGGCCCGGCGCACGGCGGA
GGCCCGGCCGTGA

Upstream 100 bases:

>100_bases
TGACGAGAAGATTTATGACCGTTTCGACCGGATGGTTCGGGTGCGCGATGGGGTCCTTGAAAGCGGTTCGGCTTGAAAGC
GCCTTGAATAGACCTCTAGA

Downstream 100 bases:

>100_bases
CCCTTGCCCCGGTCAGGGCATGTGTCTTCGATGCCTATGGCACCTTGTTCGACCTGGGCAGCCTGACCCGGTCGGTGCGT
GGTGAACTGGGCGAGCGCGC

Product: soluble lytic murein transglycosylase and related regulatory protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MPALLNNRPGFQPLKLFPVAIILSALLCLGAPSAARADGVGDAAIARQAIAAAKRDHFDEAERLARQSRSKALPRLVTWM
AYVSGRSGADFAQLGAFIHANPEWPMMSQMTKRAEESITAATPTAQVLAWFDSHPPTTADGGQAYARALFAAGRNEQAVK
VIRETWVNLSFGALQEKQYLNLLGEHLRYEDHWRRLDRLLWDRQETSVQRMIMKVDAGHRAVAQARLALQAGKSNPEPLI
NAVPASLRDDPGLIYERVRWRRQKDLDEDALDLLSHPSRNKVRPDLWWQERAILARRALQKGLVSRAYQAAADHGLEGGT
QYVDAEFLAGWVALRFLDDRATAVHHFTRLHEWASHPISRARAAYWAGRALEAAGDAKAKEWYTRAARYSTTYYGQLGAS
RLGDHHWPLPDEPQPTPDDVARFEARDVVAAARLLMQVGESELLRSFFIRLNDTVQTPGERALVAGLASRTGRHDLGLTV
ARRSDREGVTLVQAGWPVPDLDADETNPEKALVLALIRQESGFVADIESPAGAKGLMQLLPSTASKVAKSIGLKYHVNKL
DDPNFNVQVGSAYLRDLVGDFEGSYILALASYNAGPGRARRWIREYGDPRDANVDVVDWVEMIPFSETRNYVQRVMESVA
VYRRRLGKHVGPTLEADLKRWARRTAEARP

Sequences:

>Translated_670_residues
MPALLNNRPGFQPLKLFPVAIILSALLCLGAPSAARADGVGDAAIARQAIAAAKRDHFDEAERLARQSRSKALPRLVTWM
AYVSGRSGADFAQLGAFIHANPEWPMMSQMTKRAEESITAATPTAQVLAWFDSHPPTTADGGQAYARALFAAGRNEQAVK
VIRETWVNLSFGALQEKQYLNLLGEHLRYEDHWRRLDRLLWDRQETSVQRMIMKVDAGHRAVAQARLALQAGKSNPEPLI
NAVPASLRDDPGLIYERVRWRRQKDLDEDALDLLSHPSRNKVRPDLWWQERAILARRALQKGLVSRAYQAAADHGLEGGT
QYVDAEFLAGWVALRFLDDRATAVHHFTRLHEWASHPISRARAAYWAGRALEAAGDAKAKEWYTRAARYSTTYYGQLGAS
RLGDHHWPLPDEPQPTPDDVARFEARDVVAAARLLMQVGESELLRSFFIRLNDTVQTPGERALVAGLASRTGRHDLGLTV
ARRSDREGVTLVQAGWPVPDLDADETNPEKALVLALIRQESGFVADIESPAGAKGLMQLLPSTASKVAKSIGLKYHVNKL
DDPNFNVQVGSAYLRDLVGDFEGSYILALASYNAGPGRARRWIREYGDPRDANVDVVDWVEMIPFSETRNYVQRVMESVA
VYRRRLGKHVGPTLEADLKRWARRTAEARP
>Mature_669_residues
PALLNNRPGFQPLKLFPVAIILSALLCLGAPSAARADGVGDAAIARQAIAAAKRDHFDEAERLARQSRSKALPRLVTWMA
YVSGRSGADFAQLGAFIHANPEWPMMSQMTKRAEESITAATPTAQVLAWFDSHPPTTADGGQAYARALFAAGRNEQAVKV
IRETWVNLSFGALQEKQYLNLLGEHLRYEDHWRRLDRLLWDRQETSVQRMIMKVDAGHRAVAQARLALQAGKSNPEPLIN
AVPASLRDDPGLIYERVRWRRQKDLDEDALDLLSHPSRNKVRPDLWWQERAILARRALQKGLVSRAYQAAADHGLEGGTQ
YVDAEFLAGWVALRFLDDRATAVHHFTRLHEWASHPISRARAAYWAGRALEAAGDAKAKEWYTRAARYSTTYYGQLGASR
LGDHHWPLPDEPQPTPDDVARFEARDVVAAARLLMQVGESELLRSFFIRLNDTVQTPGERALVAGLASRTGRHDLGLTVA
RRSDREGVTLVQAGWPVPDLDADETNPEKALVLALIRQESGFVADIESPAGAKGLMQLLPSTASKVAKSIGLKYHVNKLD
DPNFNVQVGSAYLRDLVGDFEGSYILALASYNAGPGRARRWIREYGDPRDANVDVVDWVEMIPFSETRNYVQRVMESVAV
YRRRLGKHVGPTLEADLKRWARRTAEARP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=157, Percent_Identity=38.2165605095541, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74689; Mature: 74558

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALLNNRPGFQPLKLFPVAIILSALLCLGAPSAARADGVGDAAIARQAIAAAKRDHFDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
AERLARQSRSKALPRLVTWMAYVSGRSGADFAQLGAFIHANPEWPMMSQMTKRAEESITA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHEECCCCCCHHHHHHHHHHHHHHC
ATPTAQVLAWFDSHPPTTADGGQAYARALFAAGRNEQAVKVIRETWVNLSFGALQEKQYL
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
NLLGEHLRYEDHWRRLDRLLWDRQETSVQRMIMKVDAGHRAVAQARLALQAGKSNPEPLI
HHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHH
NAVPASLRDDPGLIYERVRWRRQKDLDEDALDLLSHPSRNKVRPDLWWQERAILARRALQ
HHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KGLVSRAYQAAADHGLEGGTQYVDAEFLAGWVALRFLDDRATAVHHFTRLHEWASHPISR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ARAAYWAGRALEAAGDAKAKEWYTRAARYSTTYYGQLGASRLGDHHWPLPDEPQPTPDDV
HHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCCCCCCCCHHHH
ARFEARDVVAAARLLMQVGESELLRSFFIRLNDTVQTPGERALVAGLASRTGRHDLGLTV
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEE
ARRSDREGVTLVQAGWPVPDLDADETNPEKALVLALIRQESGFVADIESPAGAKGLMQLL
ECCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHH
PSTASKVAKSIGLKYHVNKLDDPNFNVQVGSAYLRDLVGDFEGSYILALASYNAGPGRAR
HHHHHHHHHHCCCEEEECCCCCCCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
RWIREYGDPRDANVDVVDWVEMIPFSETRNYVQRVMESVAVYRRRLGKHVGPTLEADLKR
HHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WARRTAEARP
HHHHHCCCCC
>Mature Secondary Structure 
PALLNNRPGFQPLKLFPVAIILSALLCLGAPSAARADGVGDAAIARQAIAAAKRDHFDE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
AERLARQSRSKALPRLVTWMAYVSGRSGADFAQLGAFIHANPEWPMMSQMTKRAEESITA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHEECCCCCCHHHHHHHHHHHHHHC
ATPTAQVLAWFDSHPPTTADGGQAYARALFAAGRNEQAVKVIRETWVNLSFGALQEKQYL
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
NLLGEHLRYEDHWRRLDRLLWDRQETSVQRMIMKVDAGHRAVAQARLALQAGKSNPEPLI
HHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHH
NAVPASLRDDPGLIYERVRWRRQKDLDEDALDLLSHPSRNKVRPDLWWQERAILARRALQ
HHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KGLVSRAYQAAADHGLEGGTQYVDAEFLAGWVALRFLDDRATAVHHFTRLHEWASHPISR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ARAAYWAGRALEAAGDAKAKEWYTRAARYSTTYYGQLGASRLGDHHWPLPDEPQPTPDDV
HHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCCCCCCCCHHHH
ARFEARDVVAAARLLMQVGESELLRSFFIRLNDTVQTPGERALVAGLASRTGRHDLGLTV
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEE
ARRSDREGVTLVQAGWPVPDLDADETNPEKALVLALIRQESGFVADIESPAGAKGLMQLL
ECCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHH
PSTASKVAKSIGLKYHVNKLDDPNFNVQVGSAYLRDLVGDFEGSYILALASYNAGPGRAR
HHHHHHHHHHCCCEEEECCCCCCCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
RWIREYGDPRDANVDVVDWVEMIPFSETRNYVQRVMESVAVYRRRLGKHVGPTLEADLKR
HHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WARRTAEARP
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]