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The map label for this gene is rsuA [H]

Identifier: 83311576

GI number: 83311576

Start: 2656179

End: 2656922

Strand: Reverse

Name: rsuA [H]

Synonym: amb2477

Alternate gene names: 83311576

Gene position: 2656922-2656179 (Counterclockwise)

Preceding gene: 83311577

Following gene: 83311571

Centisome position: 53.49

GC content: 68.82

Gene sequence:

>744_bases
ATGCGGCACGCATCCCGCCCGGCGCCTGAGGGGCATACGGATATGCGCCTGATCCGCCTCATCGCCAATCTGGGCTATGG
CAGCCAGCGCGACGTCAAGGCCATGATCAAGGCCGGACGCGTTACCCTGGCCGACGGCACGGTGCTGGACGACGGCACCA
AGGCCGCCCACGACGACATCCGGGTGGATGGCGAGCCGCTGGATCCGCCTCCCGGTCTGGTGCTGATGCTCCATAAGCCC
GAGGGCTATACCTGCTCTACCTCGGACCCGGGACGCATCGTCTACGAATTGCTGCCCGAGCGCTTCATGCACCGCAATCC
CATCGTGGCGCCGGTGGGCCGCCTGGATCGGGACAGCACCGGCCTGCTGCTGCTGACCGACGACGGGCCGCTGGCGCACC
GCATCACCTCGCCCAAGCGCCATGTACCCAAGACCTACGAGGTGACATTGGCCCGGCCTCTGAGCGGGGAGGAGGGGGCG
GTGTTCGCCTCCGGCACCCTGATGCTGCGCTCGGAATCCACGCCCCTGGCTCCGGCGGAACTGGTGGTCAGCGGCCCGCA
GACGGCGCGCATCACCATCACCGAGGGGCGCTACCATCAGGTCAAGCGCATGTTCGCCGCCGTCGGCAACCATGTGGAGC
GCCTGCACCGCGCCGCCATCGGCGGGCTGGATCTGGGCGATCTGGCGGAAGGGCAGTGGCGGCCGCTCAGCCCGGCCGAG
ATTGCCCTGGTGACAGGGTCGTAA

Upstream 100 bases:

>100_bases
AGTGACGCTGCTGCGCGAGGCCGACGGCTTTAAGGTCTATCTGGCCAGCAACCGCCATGACAAATAATGACGATCACCGA
CAAGCCGCAGAAGCTAAGAG

Downstream 100 bases:

>100_bases
GGCTATCTTTGATCAGGCCGAAGAAAATTCGGGGTGCCTCCGCGCCCTCGAAGCGCCAGCCCAGCTGCAGGTCGCAGCCG
GTACAGGCGGCCAGCCGCCA

Product: 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRHASRPAPEGHTDMRLIRLIANLGYGSQRDVKAMIKAGRVTLADGTVLDDGTKAAHDDIRVDGEPLDPPPGLVLMLHKP
EGYTCSTSDPGRIVYELLPERFMHRNPIVAPVGRLDRDSTGLLLLTDDGPLAHRITSPKRHVPKTYEVTLARPLSGEEGA
VFASGTLMLRSESTPLAPAELVVSGPQTARITITEGRYHQVKRMFAAVGNHVERLHRAAIGGLDLGDLAEGQWRPLSPAE
IALVTGS

Sequences:

>Translated_247_residues
MRHASRPAPEGHTDMRLIRLIANLGYGSQRDVKAMIKAGRVTLADGTVLDDGTKAAHDDIRVDGEPLDPPPGLVLMLHKP
EGYTCSTSDPGRIVYELLPERFMHRNPIVAPVGRLDRDSTGLLLLTDDGPLAHRITSPKRHVPKTYEVTLARPLSGEEGA
VFASGTLMLRSESTPLAPAELVVSGPQTARITITEGRYHQVKRMFAAVGNHVERLHRAAIGGLDLGDLAEGQWRPLSPAE
IALVTGS
>Mature_247_residues
MRHASRPAPEGHTDMRLIRLIANLGYGSQRDVKAMIKAGRVTLADGTVLDDGTKAAHDDIRVDGEPLDPPPGLVLMLHKP
EGYTCSTSDPGRIVYELLPERFMHRNPIVAPVGRLDRDSTGLLLLTDDGPLAHRITSPKRHVPKTYEVTLARPLSGEEGA
VFASGTLMLRSESTPLAPAELVVSGPQTARITITEGRYHQVKRMFAAVGNHVERLHRAAIGGLDLGDLAEGQWRPLSPAE
IALVTGS

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=45.9574468085106, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787524, Length=240, Percent_Identity=33.75, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=30.6382978723404, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26701; Mature: 26701

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHASRPAPEGHTDMRLIRLIANLGYGSQRDVKAMIKAGRVTLADGTVLDDGTKAAHDDI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCEEECCCCCCCCCCE
RVDGEPLDPPPGLVLMLHKPEGYTCSTSDPGRIVYELLPERFMHRNPIVAPVGRLDRDST
EECCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GLLLLTDDGPLAHRITSPKRHVPKTYEVTLARPLSGEEGAVFASGTLMLRSESTPLAPAE
CEEEEECCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCEEEECCEEEEECCCCCCCCCE
LVVSGPQTARITITEGRYHQVKRMFAAVGNHVERLHRAAIGGLDLGDLAEGQWRPLSPAE
EEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
IALVTGS
EEEEECC
>Mature Secondary Structure
MRHASRPAPEGHTDMRLIRLIANLGYGSQRDVKAMIKAGRVTLADGTVLDDGTKAAHDDI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCEEECCCCCCCCCCE
RVDGEPLDPPPGLVLMLHKPEGYTCSTSDPGRIVYELLPERFMHRNPIVAPVGRLDRDST
EECCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GLLLLTDDGPLAHRITSPKRHVPKTYEVTLARPLSGEEGAVFASGTLMLRSESTPLAPAE
CEEEEECCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCEEEECCEEEEECCCCCCCCCE
LVVSGPQTARITITEGRYHQVKRMFAAVGNHVERLHRAAIGGLDLGDLAEGQWRPLSPAE
EEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
IALVTGS
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]