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The map label for this gene is braE [H]

Identifier: 83311558

GI number: 83311558

Start: 2635463

End: 2636455

Strand: Reverse

Name: braE [H]

Synonym: amb2459

Alternate gene names: 83311558

Gene position: 2636455-2635463 (Counterclockwise)

Preceding gene: 83311559

Following gene: 83311557

Centisome position: 53.08

GC content: 66.97

Gene sequence:

>993_bases
ATGAGCCGCCAAGCCATTCTGCTTTGCCTGTTGCTGGCCGCCTTCGCCCTGGCCCCGCTGGCGGCCGGGCCTTACCTGCT
GTCGGTGCTGGTGGTGGTGATGTATTTCGCCTATGTGGGCCAGGCGTGGAACATCATGATGGGCTTCGCCGGGCAATTGT
CCCTGGGCCACACCCTCTATGTGGGGCTCGGCGCCTATACGGCGGGGGCGCTGTTCGTCCATCTGGGCGTGCCGGCGGCC
CTGGGGCTGTTCGCGGCGGTGGCGGCGGCCGTGCTGGTGGGGGGAGCCGTCGGGGCGCTTGGGTTCCGCTTCGGCGTCAA
GGGCGTCTATTTCGCCCTGCTGACCATCGCCTTCGCCGAGTTCACCCGCATCCTGTTCGAGCACATTCCCGGCCTGGGCG
GTCCCGGCGGCCTGTTCCTGCCGGTGGGGGACCGCACCGGAATCGACCTGATCCATCTGCGCGGCCATCCCTTGATGTTC
TACTACCTGATCCTGGCCATGACCGTGGGCATCTTCCTGCTGTGTCGGAAGCTGGTGCGGTCGCGCCTCGGCTACACTTG
GCTGGCGGTGCGCGAGGACCAGGAGGCGGCGGCGGCGCTGGGCATCAACGTGTTCCGCGCCAAGCTTTATGCCGTCATGC
TGTCGGCCGGACTGACCGCGCTGGGCGGCGTGTTCTTCGCCTTCTACTACAACAACCTGTTCCCCAATCAGGCCTTCGGC
ATGAACCGGTCCATCGAGATCATCCTGGCGCCCATCGTCGGGGGCCTGGGAACCCTGTTCGGGCCGATCCTCGGGGCGCT
GATCCTGACGCCCCTGGGCGAGGGGATCACCATGGCCACCGAGGCCCTGGGATTCAAGGCGGCAGGCATCAAGCAGCTGT
GCTACGGCCTGTCGCTGCTGGCCATCGTCAAGTTCCTGCCCGACGGCGTTTGGCCCTGGATTCGGACCCGTCTGGGGCTG
GAACCCGAGGAAGACGGGGAGGGCGACCGTTGA

Upstream 100 bases:

>100_bases
CCGGCTATTTCCTGATGCCCTCCATGAAGGCCATGTTCAGCTTCGCCATCCTGATCCTGATCCTGGTGCTGCGGCCCCAG
GGGCTGATGGGGGCGCGCAA

Downstream 100 bases:

>100_bases
GCGCCGCGCCGTTGCTGCTGGTCGAAGGGCTGGTCAAGAACTTCCGGGGCCTGCGCGCCGTGGACGGCGTGTCGTTCAGC
GTCGCCGCTGGCGAGGTCGT

Product: ABC-type branched-chain amino acid transport system, permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSRQAILLCLLLAAFALAPLAAGPYLLSVLVVVMYFAYVGQAWNIMMGFAGQLSLGHTLYVGLGAYTAGALFVHLGVPAA
LGLFAAVAAAVLVGGAVGALGFRFGVKGVYFALLTIAFAEFTRILFEHIPGLGGPGGLFLPVGDRTGIDLIHLRGHPLMF
YYLILAMTVGIFLLCRKLVRSRLGYTWLAVREDQEAAAALGINVFRAKLYAVMLSAGLTALGGVFFAFYYNNLFPNQAFG
MNRSIEIILAPIVGGLGTLFGPILGALILTPLGEGITMATEALGFKAAGIKQLCYGLSLLAIVKFLPDGVWPWIRTRLGL
EPEEDGEGDR

Sequences:

>Translated_330_residues
MSRQAILLCLLLAAFALAPLAAGPYLLSVLVVVMYFAYVGQAWNIMMGFAGQLSLGHTLYVGLGAYTAGALFVHLGVPAA
LGLFAAVAAAVLVGGAVGALGFRFGVKGVYFALLTIAFAEFTRILFEHIPGLGGPGGLFLPVGDRTGIDLIHLRGHPLMF
YYLILAMTVGIFLLCRKLVRSRLGYTWLAVREDQEAAAALGINVFRAKLYAVMLSAGLTALGGVFFAFYYNNLFPNQAFG
MNRSIEIILAPIVGGLGTLFGPILGALILTPLGEGITMATEALGFKAAGIKQLCYGLSLLAIVKFLPDGVWPWIRTRLGL
EPEEDGEGDR
>Mature_329_residues
SRQAILLCLLLAAFALAPLAAGPYLLSVLVVVMYFAYVGQAWNIMMGFAGQLSLGHTLYVGLGAYTAGALFVHLGVPAAL
GLFAAVAAAVLVGGAVGALGFRFGVKGVYFALLTIAFAEFTRILFEHIPGLGGPGGLFLPVGDRTGIDLIHLRGHPLMFY
YLILAMTVGIFLLCRKLVRSRLGYTWLAVREDQEAAAALGINVFRAKLYAVMLSAGLTALGGVFFAFYYNNLFPNQAFGM
NRSIEIILAPIVGGLGTLFGPILGALILTPLGEGITMATEALGFKAAGIKQLCYGLSLLAIVKFLPDGVWPWIRTRLGLE
PEEDGEGDR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=326, Percent_Identity=26.9938650306748, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35036; Mature: 34905

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQAILLCLLLAAFALAPLAAGPYLLSVLVVVMYFAYVGQAWNIMMGFAGQLSLGHTLY
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VGLGAYTAGALFVHLGVPAALGLFAAVAAAVLVGGAVGALGFRFGVKGVYFALLTIAFAE
EEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTRILFEHIPGLGGPGGLFLPVGDRTGIDLIHLRGHPLMFYYLILAMTVGIFLLCRKLVR
HHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
SRLGYTWLAVREDQEAAAALGINVFRAKLYAVMLSAGLTALGGVFFAFYYNNLFPNQAFG
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MNRSIEIILAPIVGGLGTLFGPILGALILTPLGEGITMATEALGFKAAGIKQLCYGLSLL
CCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
AIVKFLPDGVWPWIRTRLGLEPEEDGEGDR
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRQAILLCLLLAAFALAPLAAGPYLLSVLVVVMYFAYVGQAWNIMMGFAGQLSLGHTLY
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VGLGAYTAGALFVHLGVPAALGLFAAVAAAVLVGGAVGALGFRFGVKGVYFALLTIAFAE
EEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTRILFEHIPGLGGPGGLFLPVGDRTGIDLIHLRGHPLMFYYLILAMTVGIFLLCRKLVR
HHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
SRLGYTWLAVREDQEAAAALGINVFRAKLYAVMLSAGLTALGGVFFAFYYNNLFPNQAFG
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MNRSIEIILAPIVGGLGTLFGPILGALILTPLGEGITMATEALGFKAAGIKQLCYGLSLL
CCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
AIVKFLPDGVWPWIRTRLGLEPEEDGEGDR
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]