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The map label for this gene is nhaA

Identifier: 83311544

GI number: 83311544

Start: 2621271

End: 2622422

Strand: Reverse

Name: nhaA

Synonym: amb2445

Alternate gene names: 83311544

Gene position: 2622422-2621271 (Counterclockwise)

Preceding gene: 83311545

Following gene: 83311543

Centisome position: 52.8

GC content: 63.54

Gene sequence:

>1152_bases
ATGGCGGCTGAGAAGCCTTCTTCCATTTTTCGGGACTTCCTGGACAGCGAGGCGGCCGGCGGCGTCATTCTGATGGTTAC
GGCGGCCTTGGCGCTGGTCATCGCCAATTCGCCGCTTTCAGGCGCCTATTTCGCCTTCTTGCAGGCCAAGTTCCTCGGCA
TGAGCGTGCTGCATGGAATCAATGACGGACTGATGGCGGTGTTCTTCCTGCTGGTCGGTCTGGAAATCAAGCGCGAGGTG
CTGGGCGGACAATTGTCCAATTGGTCGCAGCGCATCCTTCCCGGATTGGCCGCCCTGGGTGGCATGGTGGTGCCGGCCCT
GGTTTTCCTGGCCCTCAACGCCAAATCGCCGGAGACCGTGCGGGGGTGGGCCGTTCCCACCGCCACCGATATCGCCTTTG
CCTTGGGCGTCCTGGCTCTTCTGGGGCCGAGAGTGCCGGCCTCCCTCAAGATTTTCCTGACGGCGCTGGCCATCATCGAT
GACCTCGGCGCCGTCCTGGTTATCGCCTTGTTCTATACCGCCAAACTTTCGTGGCCGGCCCTGGTGGCCGTGGCGGCGAT
CCTGGCTCTCCTGGCGGCGCTCAACCGTCTTCGAGTCAGGTCCCTTTGGCCCTATCTGCTGGTGGGGGCGGGGCTATGGG
GCGCCATGTTGCAGTCGGGCGTCCATGCCACCGTCGCCGGAATTGCCCTGGCCCTGACCATTCCCATGGGCGACGAGCAG
CACTCGCCGCTGCATCGGCTCGAGCATGGTCTGGCCCCATGGGTCGGATACGGCATCGTGCCGATCTTTGGGTTTGCCAA
TGCCGGTGTGTCGTTCGCGGGATTGGAGCCATCACGGGTCCTGCAATCGTTACCGCTGGGAATCGCTCTAGGCCTGCTTT
TTGGAAAGCAGATCGGCGTATTCGGCACGGCCTGGATGGCCATCTGGCTTGGGTTCGCCGCGAGGCCCGAGGGTGCCGGC
ACGGCCCAGCTCTACGGCGTTGCCGTCCTGTGCGGCATCGGCTTCACCATGAGCCTGTTCATTGGAGCGCTGGCCTTCGG
CGAACTCCCAGCATCGTCCGACGCGGTGAAAGTCGGCGTCTTGGCGGGCTCCGCGCTTTCGGCCATCCTGGGCTCCCTCG
TACTTTTGAGGTGCCGATCTTCGTCGTCATAA

Upstream 100 bases:

>100_bases
GCCTATATCACTCATATGGGCTCCCGGCTCGATTACGAGGCGGTGCGGCGGGCTGTTCCGCCACACGTCATTCCAGCCCA
CGACGGACTGGTTATCGAGC

Downstream 100 bases:

>100_bases
TATACAAACCCGCGCAATCGCCTGCACGATGTTCGCTGATTGTGCTAAACGGCTCTATCCAGTAACAATAAATAGCTAGC
GCGTTGATTGTGGCAAGACT

Product: Na+/H+ antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAAEKPSSIFRDFLDSEAAGGVILMVTAALALVIANSPLSGAYFAFLQAKFLGMSVLHGINDGLMAVFFLLVGLEIKREV
LGGQLSNWSQRILPGLAALGGMVVPALVFLALNAKSPETVRGWAVPTATDIAFALGVLALLGPRVPASLKIFLTALAIID
DLGAVLVIALFYTAKLSWPALVAVAAILALLAALNRLRVRSLWPYLLVGAGLWGAMLQSGVHATVAGIALALTIPMGDEQ
HSPLHRLEHGLAPWVGYGIVPIFGFANAGVSFAGLEPSRVLQSLPLGIALGLLFGKQIGVFGTAWMAIWLGFAARPEGAG
TAQLYGVAVLCGIGFTMSLFIGALAFGELPASSDAVKVGVLAGSALSAILGSLVLLRCRSSSS

Sequences:

>Translated_383_residues
MAAEKPSSIFRDFLDSEAAGGVILMVTAALALVIANSPLSGAYFAFLQAKFLGMSVLHGINDGLMAVFFLLVGLEIKREV
LGGQLSNWSQRILPGLAALGGMVVPALVFLALNAKSPETVRGWAVPTATDIAFALGVLALLGPRVPASLKIFLTALAIID
DLGAVLVIALFYTAKLSWPALVAVAAILALLAALNRLRVRSLWPYLLVGAGLWGAMLQSGVHATVAGIALALTIPMGDEQ
HSPLHRLEHGLAPWVGYGIVPIFGFANAGVSFAGLEPSRVLQSLPLGIALGLLFGKQIGVFGTAWMAIWLGFAARPEGAG
TAQLYGVAVLCGIGFTMSLFIGALAFGELPASSDAVKVGVLAGSALSAILGSLVLLRCRSSSS
>Mature_382_residues
AAEKPSSIFRDFLDSEAAGGVILMVTAALALVIANSPLSGAYFAFLQAKFLGMSVLHGINDGLMAVFFLLVGLEIKREVL
GGQLSNWSQRILPGLAALGGMVVPALVFLALNAKSPETVRGWAVPTATDIAFALGVLALLGPRVPASLKIFLTALAIIDD
LGAVLVIALFYTAKLSWPALVAVAAILALLAALNRLRVRSLWPYLLVGAGLWGAMLQSGVHATVAGIALALTIPMGDEQH
SPLHRLEHGLAPWVGYGIVPIFGFANAGVSFAGLEPSRVLQSLPLGIALGLLFGKQIGVFGTAWMAIWLGFAARPEGAGT
AQLYGVAVLCGIGFTMSLFIGALAFGELPASSDAVKVGVLAGSALSAILGSLVLLRCRSSSS

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=383, Percent_Identity=51.1749347258486, Blast_Score=308, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_MAGSA (Q2W4H6)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421808.1
- ProteinModelPortal:   Q2W4H6
- SMR:   Q2W4H6
- STRING:   Q2W4H6
- GeneID:   3806488
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2445
- NMPDR:   fig|342108.5.peg.2147
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   YFALRDL
- PhylomeDB:   Q2W4H6
- BioCyc:   MMAG342108:AMB2445-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 39533; Mature: 39402

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x40407f30)-; HASH(0xb60e0b0)-; HASH(0x13064cb0)-; HASH(0x12e2afe8)-; HASH(0x11551318)-; HASH(0x12f311e8)-; HASH(0xb536e38)-; HASH(0x12b2ca68)-; HASH(0x12c20f9c)-; HASH(0x134a187c)-; HASH(0x4040a264)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEKPSSIFRDFLDSEAAGGVILMVTAALALVIANSPLSGAYFAFLQAKFLGMSVLHGI
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
NDGLMAVFFLLVGLEIKREVLGGQLSNWSQRILPGLAALGGMVVPALVFLALNAKSPETV
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RGWAVPTATDIAFALGVLALLGPRVPASLKIFLTALAIIDDLGAVLVIALFYTAKLSWPA
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LVAVAAILALLAALNRLRVRSLWPYLLVGAGLWGAMLQSGVHATVAGIALALTIPMGDEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCC
HSPLHRLEHGLAPWVGYGIVPIFGFANAGVSFAGLEPSRVLQSLPLGIALGLLFGKQIGV
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHH
FGTAWMAIWLGFAARPEGAGTAQLYGVAVLCGIGFTMSLFIGALAFGELPASSDAVKVGV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEH
LAGSALSAILGSLVLLRCRSSSS
HHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
AAEKPSSIFRDFLDSEAAGGVILMVTAALALVIANSPLSGAYFAFLQAKFLGMSVLHGI
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
NDGLMAVFFLLVGLEIKREVLGGQLSNWSQRILPGLAALGGMVVPALVFLALNAKSPETV
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RGWAVPTATDIAFALGVLALLGPRVPASLKIFLTALAIIDDLGAVLVIALFYTAKLSWPA
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LVAVAAILALLAALNRLRVRSLWPYLLVGAGLWGAMLQSGVHATVAGIALALTIPMGDEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCC
HSPLHRLEHGLAPWVGYGIVPIFGFANAGVSFAGLEPSRVLQSLPLGIALGLLFGKQIGV
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHH
FGTAWMAIWLGFAARPEGAGTAQLYGVAVLCGIGFTMSLFIGALAFGELPASSDAVKVGV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEH
LAGSALSAILGSLVLLRCRSSSS
HHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA