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The map label for this gene is rep [H]

Identifier: 83311537

GI number: 83311537

Start: 2618220

End: 2619482

Strand: Reverse

Name: rep [H]

Synonym: amb2438

Alternate gene names: 83311537

Gene position: 2619482-2618220 (Counterclockwise)

Preceding gene: 83311538

Following gene: 83311536

Centisome position: 52.74

GC content: 69.6

Gene sequence:

>1263_bases
ATGACCCCTCTCGGGAACATCGCAAGTAATGTAGCCCCCCCCCTCCCCGGAGGCGAAGCCGGAGCCGGAGGGGGGCCCCT
GGCTGAGCCTGTCCGCCGGCTCAAGTGGCGTCTCCGCGACCACGCCGGGCGGATGCTCCCCAAGCAGTCCATTCCTGGCC
TGGATGGCGTCACCCGCGAGCGCCTGCACCGGGTGGCTCGATGTGGCCGCCACATCCTCGGCGGACAGGTGGTCGTGGTG
GCGGGGGAGGGCGGCGCCCGGTTCCTCGGCCTGGAAACATGCGGCTCCGTGTGGCTGTGCCCGGTTTGCTCGTCCAAGAT
CGCTGAGCACCGCCGCGCCGACATCGAAACCATGTTGACGGCCCATTGCGCGGGCCAGCGGCGGCGGTTCACCAAGGCCC
CTGGAGACACCTGGGGCGGGGCCTGGGAAACCTATGAGGCGGCTCCCGGCGCGGTCTACATGGCCACCTTCACCGTGCCC
CATGGCCGGTGGGATAGCCTGGAACGCCTGCGCGACGACGTGGCCGAGTGCTGGCGCCGCGTGCAACGTGGGGCGGCCTG
GGCGCGTCTCAAGGCCCGCTGTGGCATCGTGGGCGTGATCCGGGCCATGGAACTGACCCACGGCGCCAACGGATGGCACC
CCCATCTGCATGTGCTGATCCTGGCAAAGCCGGGGGCCTGCGTCGAAATGACCCTGCGCCATGCCCTCTATGCCCGCTGG
GCGGCCTTCGTCGGCAAGATGGGCCTGGGGGTGGTGTCGGAAACCCATGGGGTGGACCTGTACCGCGCCCATTCCGTCCA
AGCCGCCGGTGACTACGTCGCCAAGTGGGGCTGCGACGCCGAGTTGACCAAATGGCACGTCAAGAAGGGGCGGGGAAAGA
ACCGCTCCCCCTGGCAACTGCTGGCCGCCGCCGCCGAGGGCGACGTTGAGGCCCGCATCCGGTGGCGCGAGTTCGCCGCC
GTCATGCCGGGGACCTGCCACATCACCATGAGCCACGGCCTGCGCGACCTGTACTTGGACGGTCCCGACCTGTCCGACGC
CGAGATCGCCGCCATGGAGCATGGCATCGACCCCGCCGAGATCGTTCGGAATACCGGGGAAACCATTGTCGGGCATATCC
GGCGGGGCGTGTGGGTCCGCGTCATGCGGGCCGGGCTCGCCGTGGACGTGCTGGAAGCCGTGGAAACGGGCGGGTGGGCC
GCCGCCCTTTCCATGCTGGTGGATCGTGGCCTTGCACTGAGGAGGGCCAGCGATGGCGGATGA

Upstream 100 bases:

>100_bases
AACTTGTTGGCGTGATTGTTCTGGCCTTGTTCGCCGTGGCGTTCCCGCGCTTCGGTGTGGGGATCTGGATTGGGCTGTTC
ATCGGTTGGGGGCTGTGGTC

Downstream 100 bases:

>100_bases
GGAAACGAGGTCGGGGACGGTGGTTGGGTATGGCCCGTGTAAGTTCTGTGGGAACGAGGGCAAGTATCAGGTCAACAAGC
GCGGGAAATTGTATTTCTAC

Product: Rep protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTPLGNIASNVAPPLPGGEAGAGGGPLAEPVRRLKWRLRDHAGRMLPKQSIPGLDGVTRERLHRVARCGRHILGGQVVVV
AGEGGARFLGLETCGSVWLCPVCSSKIAEHRRADIETMLTAHCAGQRRRFTKAPGDTWGGAWETYEAAPGAVYMATFTVP
HGRWDSLERLRDDVAECWRRVQRGAAWARLKARCGIVGVIRAMELTHGANGWHPHLHVLILAKPGACVEMTLRHALYARW
AAFVGKMGLGVVSETHGVDLYRAHSVQAAGDYVAKWGCDAELTKWHVKKGRGKNRSPWQLLAAAAEGDVEARIRWREFAA
VMPGTCHITMSHGLRDLYLDGPDLSDAEIAAMEHGIDPAEIVRNTGETIVGHIRRGVWVRVMRAGLAVDVLEAVETGGWA
AALSMLVDRGLALRRASDGG

Sequences:

>Translated_420_residues
MTPLGNIASNVAPPLPGGEAGAGGGPLAEPVRRLKWRLRDHAGRMLPKQSIPGLDGVTRERLHRVARCGRHILGGQVVVV
AGEGGARFLGLETCGSVWLCPVCSSKIAEHRRADIETMLTAHCAGQRRRFTKAPGDTWGGAWETYEAAPGAVYMATFTVP
HGRWDSLERLRDDVAECWRRVQRGAAWARLKARCGIVGVIRAMELTHGANGWHPHLHVLILAKPGACVEMTLRHALYARW
AAFVGKMGLGVVSETHGVDLYRAHSVQAAGDYVAKWGCDAELTKWHVKKGRGKNRSPWQLLAAAAEGDVEARIRWREFAA
VMPGTCHITMSHGLRDLYLDGPDLSDAEIAAMEHGIDPAEIVRNTGETIVGHIRRGVWVRVMRAGLAVDVLEAVETGGWA
AALSMLVDRGLALRRASDGG
>Mature_419_residues
TPLGNIASNVAPPLPGGEAGAGGGPLAEPVRRLKWRLRDHAGRMLPKQSIPGLDGVTRERLHRVARCGRHILGGQVVVVA
GEGGARFLGLETCGSVWLCPVCSSKIAEHRRADIETMLTAHCAGQRRRFTKAPGDTWGGAWETYEAAPGAVYMATFTVPH
GRWDSLERLRDDVAECWRRVQRGAAWARLKARCGIVGVIRAMELTHGANGWHPHLHVLILAKPGACVEMTLRHALYARWA
AFVGKMGLGVVSETHGVDLYRAHSVQAAGDYVAKWGCDAELTKWHVKKGRGKNRSPWQLLAAAAEGDVEARIRWREFAAV
MPGTCHITMSHGLRDLYLDGPDLSDAEIAAMEHGIDPAEIVRNTGETIVGHIRRGVWVRVMRAGLAVDVLEAVETGGWAA
ALSMLVDRGLALRRASDGG

Specific function: Essential for the autonomous replication of the plasmid pIJ101 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-positive plasmids replication protein type 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45525; Mature: 45394

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLGNIASNVAPPLPGGEAGAGGGPLAEPVRRLKWRLRDHAGRMLPKQSIPGLDGVTRE
CCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RLHRVARCGRHILGGQVVVVAGEGGARFLGLETCGSVWLCPVCSSKIAEHRRADIETMLT
HHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHCCCEEECHHHHHHHHHHHHHHHHHHHH
AHCAGQRRRFTKAPGDTWGGAWETYEAAPGAVYMATFTVPHGRWDSLERLRDDVAECWRR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
VQRGAAWARLKARCGIVGVIRAMELTHGANGWHPHLHVLILAKPGACVEMTLRHALYARW
HHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
AAFVGKMGLGVVSETHGVDLYRAHSVQAAGDYVAKWGCDAELTKWHVKKGRGKNRSPWQL
HHHHHHHCCCCCCCCCCCEEEECCCCHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCHHHH
LAAAAEGDVEARIRWREFAAVMPGTCHITMSHGLRDLYLDGPDLSDAEIAAMEHGIDPAE
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCHHH
IVRNTGETIVGHIRRGVWVRVMRAGLAVDVLEAVETGGWAAALSMLVDRGLALRRASDGG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHEECCCCCC
>Mature Secondary Structure 
TPLGNIASNVAPPLPGGEAGAGGGPLAEPVRRLKWRLRDHAGRMLPKQSIPGLDGVTRE
CCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RLHRVARCGRHILGGQVVVVAGEGGARFLGLETCGSVWLCPVCSSKIAEHRRADIETMLT
HHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHCCCEEECHHHHHHHHHHHHHHHHHHHH
AHCAGQRRRFTKAPGDTWGGAWETYEAAPGAVYMATFTVPHGRWDSLERLRDDVAECWRR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
VQRGAAWARLKARCGIVGVIRAMELTHGANGWHPHLHVLILAKPGACVEMTLRHALYARW
HHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
AAFVGKMGLGVVSETHGVDLYRAHSVQAAGDYVAKWGCDAELTKWHVKKGRGKNRSPWQL
HHHHHHHCCCCCCCCCCCEEEECCCCHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCHHHH
LAAAAEGDVEARIRWREFAAVMPGTCHITMSHGLRDLYLDGPDLSDAEIAAMEHGIDPAE
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCHHH
IVRNTGETIVGHIRRGVWVRVMRAGLAVDVLEAVETGGWAAALSMLVDRGLALRRASDGG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3170481 [H]