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The map label for this gene is mazG

Identifier: 83311491

GI number: 83311491

Start: 2597341

End: 2598162

Strand: Reverse

Name: mazG

Synonym: amb2392

Alternate gene names: 83311491

Gene position: 2598162-2597341 (Counterclockwise)

Preceding gene: 83311492

Following gene: 83311490

Centisome position: 52.31

GC content: 65.69

Gene sequence:

>822_bases
ATGAGCCAGGACAAGCCCATCGACCGATTGCTGGCCATCATGGCCCGCCTGCGCTCCCCCCAGGGCGGCTGTCCCTGGGA
TCTGGAGCAGACCTTCGCCACCATCGCCCCCTACACCATCGAGGAAGCCTACGAGGTGGCCGAAGCCATCGAGCATGGCG
ACATGCCCCAGTTGAAGGATGAACTGGGCGACCTGCTGTTCCAGGTGGTGTTCTACGCCCAGATGGCCAAGGAGAACGGC
GATTTCGATTTCGACGCCATCGCCTCGGCCATCTCCGACAAGATGATCCGTCGCCATCCCCATGTGTTCGTCGAGGATGA
CGGCCGGGATTCCGCCGGTCAGGTGGAGGCCTGGGAGGAGACCAAGGCCCGTGAGCGTGCCGCCAAGGCGAAGGGCGCCA
ACATGGGCGTCCTGGACGGCGTGGCCCGCACCCTGCCCCCCATGACCCGCGCCCTGAAGCTGCAGAACCGCGCCGCCCGC
GTCGGCTTCGACTGGGACCGGCCCGAGACCATCCTCGACAAGCTGGCCGAGGAAGCCCAGGAAATCGCCGAGGAAATTCG
CGCCGAGGCGCCCTTCGATCGGCTGGAGGACGAGATGGGCGACGTTCTGTTCGTCTGCGTCAATCTCGCCCGCAAGCTGA
ACATCGACCCGGAACGCGCCCTGAAGCGCGCCAACGCCAAGTTCGAACGCCGCTTTCGTCATATCGAGACGGAATTGAAC
GCCACCGGCCGCACGCCCCAAGCCGCCACCCTCGACGAGATGGAGGCACTGTGGCAGGCTGCCAAGACCTTGGAGAAGGC
CGGAGGGCATGGTGGCGGGTGA

Upstream 100 bases:

>100_bases
ACGAGATCACCGAGACCTATCGCCGGCACTGCCCGTTGCTGATGACCGACGACCCCTGGGACAAGGGCGCCGATTTCGCC
GCCGAGGCCAAGGCGCCGGG

Downstream 100 bases:

>100_bases
TTGGCTGAAACGGATCGGCGTCATGGCGGCGGTATTGCTGCTGTCGTCGTGCTATATCCCCGACAAGTTTCGAAGCGAAC
TGCGCCTGTCCAAGTACGGC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSQDKPIDRLLAIMARLRSPQGGCPWDLEQTFATIAPYTIEEAYEVAEAIEHGDMPQLKDELGDLLFQVVFYAQMAKENG
DFDFDAIASAISDKMIRRHPHVFVEDDGRDSAGQVEAWEETKARERAAKAKGANMGVLDGVARTLPPMTRALKLQNRAAR
VGFDWDRPETILDKLAEEAQEIAEEIRAEAPFDRLEDEMGDVLFVCVNLARKLNIDPERALKRANAKFERRFRHIETELN
ATGRTPQAATLDEMEALWQAAKTLEKAGGHGGG

Sequences:

>Translated_273_residues
MSQDKPIDRLLAIMARLRSPQGGCPWDLEQTFATIAPYTIEEAYEVAEAIEHGDMPQLKDELGDLLFQVVFYAQMAKENG
DFDFDAIASAISDKMIRRHPHVFVEDDGRDSAGQVEAWEETKARERAAKAKGANMGVLDGVARTLPPMTRALKLQNRAAR
VGFDWDRPETILDKLAEEAQEIAEEIRAEAPFDRLEDEMGDVLFVCVNLARKLNIDPERALKRANAKFERRFRHIETELN
ATGRTPQAATLDEMEALWQAAKTLEKAGGHGGG
>Mature_272_residues
SQDKPIDRLLAIMARLRSPQGGCPWDLEQTFATIAPYTIEEAYEVAEAIEHGDMPQLKDELGDLLFQVVFYAQMAKENGD
FDFDAIASAISDKMIRRHPHVFVEDDGRDSAGQVEAWEETKARERAAKAKGANMGVLDGVARTLPPMTRALKLQNRAARV
GFDWDRPETILDKLAEEAQEIAEEIRAEAPFDRLEDEMGDVLFVCVNLARKLNIDPERALKRANAKFERRFRHIETELNA
TGRTPQAATLDEMEALWQAAKTLEKAGGHGGG

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=261, Percent_Identity=53.639846743295, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30536; Mature: 30405

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDKPIDRLLAIMARLRSPQGGCPWDLEQTFATIAPYTIEEAYEVAEAIEHGDMPQLKD
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
ELGDLLFQVVFYAQMAKENGDFDFDAIASAISDKMIRRHPHVFVEDDGRDSAGQVEAWEE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TKARERAAKAKGANMGVLDGVARTLPPMTRALKLQNRAARVGFDWDRPETILDKLAEEAQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EIAEEIRAEAPFDRLEDEMGDVLFVCVNLARKLNIDPERALKRANAKFERRFRHIETELN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ATGRTPQAATLDEMEALWQAAKTLEKAGGHGGG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQDKPIDRLLAIMARLRSPQGGCPWDLEQTFATIAPYTIEEAYEVAEAIEHGDMPQLKD
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
ELGDLLFQVVFYAQMAKENGDFDFDAIASAISDKMIRRHPHVFVEDDGRDSAGQVEAWEE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TKARERAAKAKGANMGVLDGVARTLPPMTRALKLQNRAARVGFDWDRPETILDKLAEEAQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EIAEEIRAEAPFDRLEDEMGDVLFVCVNLARKLNIDPERALKRANAKFERRFRHIETELN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ATGRTPQAATLDEMEALWQAAKTLEKAGGHGGG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]