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The map label for this gene is hisI

Identifier: 83311406

GI number: 83311406

Start: 2509409

End: 2509807

Strand: Reverse

Name: hisI

Synonym: amb2307

Alternate gene names: 83311406

Gene position: 2509807-2509409 (Counterclockwise)

Preceding gene: 83311407

Following gene: 83311405

Centisome position: 50.53

GC content: 65.66

Gene sequence:

>399_bases
ATGTCCGCCATCGACAGCGCCGCCGTTCTCGCCAAGATCAAGTTCAACGCCGACGGCCTGGTGCCCGCCATCGCCCAGCA
GCACGATACCGGCGAGGTGCTGATGATGGCCTGGATGAACGCCGAATCCGTGGCCGAGACCCTGGCGACCGGCCGGGTCT
GCTATTTCTCGCGCTCGCGCGGCGGGTTGTGGCGCAAGGGCGAGACCTCGGGCCAGCAACAGCGCCTCAAGGACTTCCTG
ATCGATTGCGACGGCGACACCATCTTGTTGAAGGTCGATCAGGACGGCGTCGCCTGCCACACCGGGCGCCGCACCTGCTT
CTACACCGCCGCCCGCCCCGAAGGCCTGACCGAGGTGGCGAAGGTGCAGGTGTCTCCCGACGAGCTTTACAAGAAGTAA

Upstream 100 bases:

>100_bases
CGGGACTCCTATGTGCAGCAGATGCGGGGACTCGCCAGCGCCCTTGCCGAGTGCCTTGCCGCCCGTTAAGGATGGTCTCC
CATCCCAAGAGGTATCGATC

Downstream 100 bases:

>100_bases
TGAATGGGGTTTGGGGCCCTCGGCCCCAAGCGGGTATGGGCGGCAGCCCATAAACTGGAGTTGCATGCATGAGTCTCATC
CCCGTCACCGTCCTGACCGG

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSAIDSAAVLAKIKFNADGLVPAIAQQHDTGEVLMMAWMNAESVAETLATGRVCYFSRSRGGLWRKGETSGQQQRLKDFL
IDCDGDTILLKVDQDGVACHTGRRTCFYTAARPEGLTEVAKVQVSPDELYKK

Sequences:

>Translated_132_residues
MSAIDSAAVLAKIKFNADGLVPAIAQQHDTGEVLMMAWMNAESVAETLATGRVCYFSRSRGGLWRKGETSGQQQRLKDFL
IDCDGDTILLKVDQDGVACHTGRRTCFYTAARPEGLTEVAKVQVSPDELYKK
>Mature_131_residues
SAIDSAAVLAKIKFNADGLVPAIAQQHDTGEVLMMAWMNAESVAETLATGRVCYFSRSRGGLWRKGETSGQQQRLKDFLI
DCDGDTILLKVDQDGVACHTGRRTCFYTAARPEGLTEVAKVQVSPDELYKK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=90, Percent_Identity=45.5555555555556, Blast_Score=92, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319819, Length=75, Percent_Identity=40, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_MAGSA (Q2W4W4)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421670.1
- ProteinModelPortal:   Q2W4W4
- SMR:   Q2W4W4
- STRING:   Q2W4W4
- GeneID:   3802581
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2307
- NMPDR:   fig|342108.5.peg.2028
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   CLSK2397223
- BioCyc:   MMAG342108:AMB2307-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 14442; Mature: 14310

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDSAAVLAKIKFNADGLVPAIAQQHDTGEVLMMAWMNAESVAETLATGRVCYFSRSR
CCCCCCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHCCEEEEEECCC
GGLWRKGETSGQQQRLKDFLIDCDGDTILLKVDQDGVACHTGRRTCFYTAARPEGLTEVA
CCCCCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCHHH
KVQVSPDELYKK
EEEECHHHHHCC
>Mature Secondary Structure 
SAIDSAAVLAKIKFNADGLVPAIAQQHDTGEVLMMAWMNAESVAETLATGRVCYFSRSR
CCCCCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHCCEEEEEECCC
GGLWRKGETSGQQQRLKDFLIDCDGDTILLKVDQDGVACHTGRRTCFYTAARPEGLTEVA
CCCCCCCCCCCHHHHHHHHEEECCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCHHH
KVQVSPDELYKK
EEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA