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The map label for this gene is hcaT [H]

Identifier: 83311379

GI number: 83311379

Start: 2480743

End: 2481903

Strand: Reverse

Name: hcaT [H]

Synonym: amb2280

Alternate gene names: 83311379

Gene position: 2481903-2480743 (Counterclockwise)

Preceding gene: 83311380

Following gene: 83311378

Centisome position: 49.97

GC content: 68.91

Gene sequence:

>1161_bases
ATGAGCCCCACCTCCGCCTCGGCGCGGCTGGGAACCTATTACGCCGCCCTGTTCGTGGCGGTGGGCATTCACATCCCCTT
CTGGCCCCTCTGGCTGCAAAGCCGGGGGATGTCGGCCTCGGAAATCGGTCTGGTCGCTGCGGCCGGCTACCTGACCCGCA
TCCTGGCCAGCCCGGTGATCGGCCATCTGGCCGACCATGGCGGCCAGCGCCGCCTGCTGATGATCCGCCTCGCCCTGGCC
GCAGGCCTGATCTGGCTGCTGTTTCCCCTGGCCAACGGCTTCTGGCCCATCCTGGTGCTGAGCGTGGTGGCCATCTTCCC
CTTCACCGGATTGGTGCCGCTGGGCGACACCCTGGCCATGATGGTGGTCAGCCGGCACGGCCTGGATTACGGGCGGGCGC
GGCTGTGGGGATCGCTGTCCTTCATCGCCGCCGCCACCTTGTTGGGCAAGGCGCTGGAATCCTGGCCGGTGGCGGTTCTG
CCCTGGCTGATGACCGGGGCCTTGCTGCTGACCGCCGCCTCCTGCCTGGGGCTGCCCGACATCAAGGTGCCACGGCACGA
GGGCAAACCGCCGTCGCTGCGTCCGGTGCTGCTCAACCCGCTGTTCCTGCTGTTCCTGGGAACCACGGCGCTGAACCAGA
TGGCCCATACCGTCTATTACGCCTTCGCCACCATCCACTGGAAGGCGGCGGGCCTGTCGGACATGGTGATCGGCGTGCTG
TGGTCCGAAGGCGTGGTAGCCGAGATCATCCTGTTCGCCGTCAGCAACCGGGTGGTCGGCCGCTTCGGCCCTGCCGCCCT
GCTGCTGGCCGCCGCCCTGGGCGGGGTGGTGCGCTGGCTGCTTCTGGGGTCAACTGCCTCGCTGCCGCTGGTAGCCCTGG
CCCAGTTGCTGCACGCCGCCACCTTCGGCTGCGCCCATCTGGGAGCCATCCATTTCATCGCGCGGGCGGTTCCCGCCGGC
CTGTCGGCCCGCACCCAGGGCATCTTCGCCGCCATGGCGATCGGACTGGCGCCCGGCCTGATCACCCCCTTCAGCGGCCG
GCTGTATGAAAACCTGGGAGGCGGTTCCTATCTGGCCATGGCCGGTCTTTCCGTCATGTCGGCGGGGTTGGCCTGGGCCC
TGATGCGCCGCTGGACCTCGGGGGCGAGAATCACCGAGTGA

Upstream 100 bases:

>100_bases
CCGATCAGCGCGAGGTGGAGTGGAAATTCCTGGCCTCCGATGCCTTTCCCCTATCGCTGGCCGATCGCGGCATCACCCTG
GCGGCCAAGCCGCTGTCGGC

Downstream 100 bases:

>100_bases
AACACTTTCCCCTCGTCCATGCGGTGGAGTAGGATCAGTCAGGGGAAATACAGTGGCGGACTTTCGGGGATGAGCGGAAA
AGACAAGACGGTCGCGCAAA

Product: major facilitator superfamily permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSPTSASARLGTYYAALFVAVGIHIPFWPLWLQSRGMSASEIGLVAAAGYLTRILASPVIGHLADHGGQRRLLMIRLALA
AGLIWLLFPLANGFWPILVLSVVAIFPFTGLVPLGDTLAMMVVSRHGLDYGRARLWGSLSFIAAATLLGKALESWPVAVL
PWLMTGALLLTAASCLGLPDIKVPRHEGKPPSLRPVLLNPLFLLFLGTTALNQMAHTVYYAFATIHWKAAGLSDMVIGVL
WSEGVVAEIILFAVSNRVVGRFGPAALLLAAALGGVVRWLLLGSTASLPLVALAQLLHAATFGCAHLGAIHFIARAVPAG
LSARTQGIFAAMAIGLAPGLITPFSGRLYENLGGGSYLAMAGLSVMSAGLAWALMRRWTSGARITE

Sequences:

>Translated_386_residues
MSPTSASARLGTYYAALFVAVGIHIPFWPLWLQSRGMSASEIGLVAAAGYLTRILASPVIGHLADHGGQRRLLMIRLALA
AGLIWLLFPLANGFWPILVLSVVAIFPFTGLVPLGDTLAMMVVSRHGLDYGRARLWGSLSFIAAATLLGKALESWPVAVL
PWLMTGALLLTAASCLGLPDIKVPRHEGKPPSLRPVLLNPLFLLFLGTTALNQMAHTVYYAFATIHWKAAGLSDMVIGVL
WSEGVVAEIILFAVSNRVVGRFGPAALLLAAALGGVVRWLLLGSTASLPLVALAQLLHAATFGCAHLGAIHFIARAVPAG
LSARTQGIFAAMAIGLAPGLITPFSGRLYENLGGGSYLAMAGLSVMSAGLAWALMRRWTSGARITE
>Mature_385_residues
SPTSASARLGTYYAALFVAVGIHIPFWPLWLQSRGMSASEIGLVAAAGYLTRILASPVIGHLADHGGQRRLLMIRLALAA
GLIWLLFPLANGFWPILVLSVVAIFPFTGLVPLGDTLAMMVVSRHGLDYGRARLWGSLSFIAAATLLGKALESWPVAVLP
WLMTGALLLTAASCLGLPDIKVPRHEGKPPSLRPVLLNPLFLLFLGTTALNQMAHTVYYAFATIHWKAAGLSDMVIGVLW
SEGVVAEIILFAVSNRVVGRFGPAALLLAAALGGVVRWLLLGSTASLPLVALAQLLHAATFGCAHLGAIHFIARAVPAGL
SARTQGIFAAMAIGLAPGLITPFSGRLYENLGGGSYLAMAGLSVMSAGLAWALMRRWTSGARITE

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=355, Percent_Identity=35.7746478873239, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 40667; Mature: 40536

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SPTSASARLGTYYAALFVAVGIHIPFWPLWLQSRGMSASEIGLVAAAGYLTRILASPVI
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
GHLADHGGQRRLLMIRLALAAGLIWLLFPLANGFWPILVLSVVAIFPFTGLVPLGDTLAM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
MVVSRHGLDYGRARLWGSLSFIAAATLLGKALESWPVAVLPWLMTGALLLTAASCLGLPD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
IKVPRHEGKPPSLRPVLLNPLFLLFLGTTALNQMAHTVYYAFATIHWKAAGLSDMVIGVL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WSEGVVAEIILFAVSNRVVGRFGPAALLLAAALGGVVRWLLLGSTASLPLVALAQLLHAA
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TFGCAHLGAIHFIARAVPAGLSARTQGIFAAMAIGLAPGLITPFSGRLYENLGGGSYLAM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHH
AGLSVMSAGLAWALMRRWTSGARITE
HHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]