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The map label for this gene is hupB [H]

Identifier: 83311280

GI number: 83311280

Start: 2364676

End: 2364954

Strand: Reverse

Name: hupB [H]

Synonym: amb2181

Alternate gene names: 83311280

Gene position: 2364954-2364676 (Counterclockwise)

Preceding gene: 83311282

Following gene: 83311279

Centisome position: 47.61

GC content: 57.35

Gene sequence:

>279_bases
ATGAACAAGTCCGAATTGATCGCCGCCGTCGCCGATGGTGCCAACATGACCAAGGCCGACGCGGCAAGTGCCATTGATGC
TGTTTTCGCAGCCATCACCGCCGCCATGAAATCGGGTGATGATGTACGTCTGGTTGGCTTTGGAACCTTCTCGGTTTCTG
CCCGTCCTGCCCGTCAAGGCAGGAATCCGCAAACTGGCAAGACCCTCACCATCGCGGCCAGCAAGTCTGCCAAATTCACC
GCCGGAAAGAGCCTCAAGGACACCATCAATGGCCGATGA

Upstream 100 bases:

>100_bases
GCGGCTCGACTTTCCTGTTTACCTTGCCGACGCCTGAGTGAATCCGTTTGATAAATGATGGCTGTTAGTCATTCCGTTTT
TGTTTGGTAGGGAGGGGCAC

Downstream 100 bases:

>100_bases
CATCAAGGCTCTGACCACCAAGATTGTTGCCGCCTACTTGGGCAGCAACACCTTGGCGGTCACAGAAATTCCGAACCTGA
TCAAGTCCGTTTATTCGTCT

Product: nucleoid DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MNKSELIAAVADGANMTKADAASAIDAVFAAITAAMKSGDDVRLVGFGTFSVSARPARQGRNPQTGKTLTIAASKSAKFT
AGKSLKDTINGR

Sequences:

>Translated_92_residues
MNKSELIAAVADGANMTKADAASAIDAVFAAITAAMKSGDDVRLVGFGTFSVSARPARQGRNPQTGKTLTIAASKSAKFT
AGKSLKDTINGR
>Mature_92_residues
MNKSELIAAVADGANMTKADAASAIDAVFAAITAAMKSGDDVRLVGFGTFSVSARPARQGRNPQTGKTLTIAASKSAKFT
AGKSLKDTINGR

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=60, Blast_Score=110, Evalue=1e-26,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=50.561797752809, Blast_Score=97, Evalue=2e-22,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=32.2222222222222, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9360; Mature: 9360

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELIAAVADGANMTKADAASAIDAVFAAITAAMKSGDDVRLVGFGTFSVSARPARQG
CCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHCC
RNPQTGKTLTIAASKSAKFTAGKSLKDTINGR
CCCCCCCEEEEEECCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MNKSELIAAVADGANMTKADAASAIDAVFAAITAAMKSGDDVRLVGFGTFSVSARPARQG
CCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHCC
RNPQTGKTLTIAASKSAKFTAGKSLKDTINGR
CCCCCCCEEEEEECCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10877760 [H]