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The map label for this gene is bcrA [H]

Identifier: 83311238

GI number: 83311238

Start: 2314769

End: 2316061

Strand: Reverse

Name: bcrA [H]

Synonym: amb2139

Alternate gene names: 83311238

Gene position: 2316061-2314769 (Counterclockwise)

Preceding gene: 83311239

Following gene: 83311237

Centisome position: 46.63

GC content: 64.89

Gene sequence:

>1293_bases
ATGCGTTGTTTCATCGGTATCGATCTGGGCTCCACCACCACCAAAGCCGTGGTGATGGATGAGAACCTCCAGATCCTGGG
ACGCGGCATCACCAATTCGCGCTCCAACTACGACACCGCGGCCGCCGTTTCCAAGCAGGAAGCGCTGATCGACACCCGCC
TGACCCTGTTCCGCCGCGCTCTGTCGAACGTGCCGGAAGTGTCGGGCAAGGTCGACGACATCCTGTCGGATCTGGAGCGG
AACTTCCGTCACGTCCAGTTCCTGGAGCAGTTGGAAGACCTGCAGAACACCTGCATCGCCAACATCAAGGGGCCGCGCTT
CGCCGGCCGCGAGAAGGTGGTGATCGAGGCCCTGGAAGGCACCTTCTCCCGCCTGCGTGACAGCTCGGCCGCCCAGTACG
CGCCGGGCGTCAAGCGCAAGTCCGACTTCTTCCGCGATCTGGCCGGCGCCGAGTTCATGAGCCACGGCGAGGCGGTGTGC
AAGGAGGCCGGTCTCGGCTTCGACCTGATCCTCAACGTCTACGACAAGTCGATCATCGAAGTGGAAAACCGTCCGCCGGC
CGGCGACATGGAGGGCAAGTTCGTCCGCGCCCTGGAAAAGGGCACCATGACCGGGTCCTCGATCCTGGCCCCGGTGCAGT
CGGCCCTGGCCATTCCGCTGGAGGAGACTTACGTGGTCGGCACCGGCTACGGCCGCGTCCGCCTGCCCTTCCCCAAGGAG
CACATCCGCTCGGAGATCCTGTGCCACGGCCTGGGCGCCCACATGATGTACCCGGACACCCGCACGGTGCTGGACATCGG
CGGCCAGGACACCAAGGGCATCCAGGTGGACCCGGTGGGCATCGTCGAGAACTTCCAGATGAACGATCGTTGCGCCGCCG
GTTGCGGCCGTTATCTGGGCTACATCGCCGACGAGATGAACATGGGCCTGCACGAGCTCGGCCCGCTGGCCATGAAGTCC
AACAAGCAGGTTCGAATCAACTCCACCTGCACCGTGTTCGCGGGCGCCGAGTTGCGTGACCGTCTGGCCCTGGGCGAAAA
GCGCGAGGACATTCTGGCCGGTCTGCACCGCGCCATCATCCTGCGCGCCATGTCCATCCTGTCGCGCGCCGGCGGCGTCA
AGGACCAGTTCACCTTCACCGGTGGCGTCGCCAAGAACGAGGCGGCGGTGCGCGAGCTGCGCAAGCTGATCAAGGAAAAC
TACGGCGACGTGACCATCAACATCGACCCGGACTCGATCTACACCGGCGCTCTCGGCGGGGCCACCTTCGCCGTCCGCGC
GGTCGTCAACTAA

Upstream 100 bases:

>100_bases
CCAACGTCAAGAACCGCCTGGAAAGCTACTTCCAGATGATCGAGCAGAAGCGCCGTGGTGGCGGTTCCAAGACCGTGGCC
GCGTAAGGGAGGGATACACA

Downstream 100 bases:

>100_bases
TCGGGAAGGGAGGAGACATCAATGTCTGAACTTATCACTACCGGTGTTGACATCGGATCGGGCTGCATCAAGACCGTGGT
CTTCAAGGTCAATGGCGACA

Product: 2-hydroxyglutaryl-CoA dehydratase activator protein

Products: NA

Alternate protein names: 3-hydroxybenzoyl-CoA reductase subunit alpha [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRCFIGIDLGSTTTKAVVMDENLQILGRGITNSRSNYDTAAAVSKQEALIDTRLTLFRRALSNVPEVSGKVDDILSDLER
NFRHVQFLEQLEDLQNTCIANIKGPRFAGREKVVIEALEGTFSRLRDSSAAQYAPGVKRKSDFFRDLAGAEFMSHGEAVC
KEAGLGFDLILNVYDKSIIEVENRPPAGDMEGKFVRALEKGTMTGSSILAPVQSALAIPLEETYVVGTGYGRVRLPFPKE
HIRSEILCHGLGAHMMYPDTRTVLDIGGQDTKGIQVDPVGIVENFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKS
NKQVRINSTCTVFAGAELRDRLALGEKREDILAGLHRAIILRAMSILSRAGGVKDQFTFTGGVAKNEAAVRELRKLIKEN
YGDVTINIDPDSIYTGALGGATFAVRAVVN

Sequences:

>Translated_430_residues
MRCFIGIDLGSTTTKAVVMDENLQILGRGITNSRSNYDTAAAVSKQEALIDTRLTLFRRALSNVPEVSGKVDDILSDLER
NFRHVQFLEQLEDLQNTCIANIKGPRFAGREKVVIEALEGTFSRLRDSSAAQYAPGVKRKSDFFRDLAGAEFMSHGEAVC
KEAGLGFDLILNVYDKSIIEVENRPPAGDMEGKFVRALEKGTMTGSSILAPVQSALAIPLEETYVVGTGYGRVRLPFPKE
HIRSEILCHGLGAHMMYPDTRTVLDIGGQDTKGIQVDPVGIVENFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKS
NKQVRINSTCTVFAGAELRDRLALGEKREDILAGLHRAIILRAMSILSRAGGVKDQFTFTGGVAKNEAAVRELRKLIKEN
YGDVTINIDPDSIYTGALGGATFAVRAVVN
>Mature_430_residues
MRCFIGIDLGSTTTKAVVMDENLQILGRGITNSRSNYDTAAAVSKQEALIDTRLTLFRRALSNVPEVSGKVDDILSDLER
NFRHVQFLEQLEDLQNTCIANIKGPRFAGREKVVIEALEGTFSRLRDSSAAQYAPGVKRKSDFFRDLAGAEFMSHGEAVC
KEAGLGFDLILNVYDKSIIEVENRPPAGDMEGKFVRALEKGTMTGSSILAPVQSALAIPLEETYVVGTGYGRVRLPFPKE
HIRSEILCHGLGAHMMYPDTRTVLDIGGQDTKGIQVDPVGIVENFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKS
NKQVRINSTCTVFAGAELRDRLALGEKREDILAGLHRAIILRAMSILSRAGGVKDQFTFTGGVAKNEAAVRELRKLIKEN
YGDVTINIDPDSIYTGALGGATFAVRAVVN

Specific function: Catalyzes the anaerobic reduction of benzoyl-CoA and 3- hydroxybenzoyl-CoA to form cyclohexa-1,5-diene-1-carbonyl-CoA and 3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA, respectively. The enzyme also reduces other benzoyl-CoA analogs with small substituents

COG id: COG1924

COG function: function code I; Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082426, Length=208, Percent_Identity=37.5, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002731
- InterPro:   IPR011954
- InterPro:   IPR008275 [H]

Pfam domain/function: PF01869 BcrAD_BadFG [H]

EC number: =1.3.99.15 [H]

Molecular weight: Translated: 46922; Mature: 46922

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFIGIDLGSTTTKAVVMDENLQILGRGITNSRSNYDTAAAVSKQEALIDTRLTLFRRA
CEEEEEEECCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LSNVPEVSGKVDDILSDLERNFRHVQFLEQLEDLQNTCIANIKGPRFAGREKVVIEALEG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
TFSRLRDSSAAQYAPGVKRKSDFFRDLAGAEFMSHGEAVCKEAGLGFDLILNVYDKSIIE
HHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
VENRPPAGDMEGKFVRALEKGTMTGSSILAPVQSALAIPLEETYVVGTGYGRVRLPFPKE
ECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHH
HIRSEILCHGLGAHMMYPDTRTVLDIGGQDTKGIQVDPVGIVENFQMNDRCAAGCGRYLG
HHHHHHHHHCCCCCEECCCCCEEEECCCCCCCCEEECCCHHEECCCCCCHHHHHHHHHHH
YIADEMNMGLHELGPLAMKSNKQVRINSTCTVFAGAELRDRLALGEKREDILAGLHRAII
HHHHHHCCCHHHHCCHHCCCCCEEEECCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHH
LRAMSILSRAGGVKDQFTFTGGVAKNEAAVRELRKLIKENYGDVTINIDPDSIYTGALGG
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCC
ATFAVRAVVN
HHEEEEEECC
>Mature Secondary Structure
MRCFIGIDLGSTTTKAVVMDENLQILGRGITNSRSNYDTAAAVSKQEALIDTRLTLFRRA
CEEEEEEECCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LSNVPEVSGKVDDILSDLERNFRHVQFLEQLEDLQNTCIANIKGPRFAGREKVVIEALEG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
TFSRLRDSSAAQYAPGVKRKSDFFRDLAGAEFMSHGEAVCKEAGLGFDLILNVYDKSIIE
HHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
VENRPPAGDMEGKFVRALEKGTMTGSSILAPVQSALAIPLEETYVVGTGYGRVRLPFPKE
ECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHH
HIRSEILCHGLGAHMMYPDTRTVLDIGGQDTKGIQVDPVGIVENFQMNDRCAAGCGRYLG
HHHHHHHHHCCCCCEECCCCCEEEECCCCCCCCEEECCCHHEECCCCCCHHHHHHHHHHH
YIADEMNMGLHELGPLAMKSNKQVRINSTCTVFAGAELRDRLALGEKREDILAGLHRAII
HHHHHHCCCHHHHCCHHCCCCCEEEECCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHH
LRAMSILSRAGGVKDQFTFTGGVAKNEAAVRELRKLIKENYGDVTINIDPDSIYTGALGG
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCC
ATFAVRAVVN
HHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9746358; 11208796 [H]