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The map label for this gene is fadD [H]

Identifier: 83311124

GI number: 83311124

Start: 2202166

End: 2204100

Strand: Reverse

Name: fadD [H]

Synonym: amb2025

Alternate gene names: 83311124

Gene position: 2204100-2202166 (Counterclockwise)

Preceding gene: 83311125

Following gene: 83311123

Centisome position: 44.37

GC content: 65.48

Gene sequence:

>1935_bases
ATGACGACGGGGTCCGTCAGGCCTATCTCGGCCTGTAGAAGACCCCGCAGGGAAGACAAGAAGAGCGGTCCTTTGAAGGC
GGCTAGGGAGGTCACTATGAATTCGGTTGCCTGGGCACCCATCAATTTCGCACCCGCGCGGGTGGAAGTGGAGAAGCTGG
AGGACGGCGCCTTCATCATCCGCTCGCCGGACAGCCTGAAGCCTTATTCGCGCTGCCTGGGCGACCTGCTGGTCCATTGG
GCCGAGCGCATCCCCAACCAGACCTTCCTGGCCCAGCGCGACGGCGACGGCTGGCGCCGGGTGACCTGGGCCGAGACCCT
GAAGAAGGTCGAAGCCATCGGCCAGTCCCTGCTGGACCGCGGGCTGAACAAGAACCGCCCGGTGATGATCCTGTCGGACA
ACAGCATCGACCACGCTTTGCTGGCCCTGGCCGCCATGCATGTGAGCGTGCCGGTCTCGCCCATCTCGCCGGCCTATTCG
CTGATCTCCCAGGATCATTCCAAGCTGCGCTACATCGCCGACCTGCTGAAGCCGGGTCTGGTCTACGCCTCCGACGGCGC
CCGCTTCGCCGATGCCCTGGTGATTCCGGAACTGGCCCGCGTCGAGGTGGTGACCAGCACCAATCCCATCGCCTGCGGCG
TGACCTTCGACACCCTGCTGGCCGCCACCCCCGGCCCCGAGGTGGAGAACGCCTTCGCCAATGTGCGGCCCACCACCGTG
GCCAAGATCCTGTTCACCTCGGGCTCCACCGACATGCCCAAGGGGGTGATCAACACCCAGAAGATGCTGTGCTCGAACCA
GCAGGCCATCCGCCAGGCCTGGCCCTTCCTGCTGGAAAGCCATCCGGTGCTGGTGGACTGGCTGCCGTGGAATCACACCT
TCGGCGGCAACCACAACTTCAACATGATGCTGCGCCACGGCGGCACGCTGTACATCGACGAGGGCAAGCCCATGCCCGGG
CTGGTGGAGAAGACCGCCGCCAACCTCAAGGAGGTTTCGCCGACGCTGTACTTCAACGTGCCGCGCGGCTTCGACATGCT
GATCCCCATGCTGGAGAAGGATGACGAGCTGCGGAACAGCTTCTTCAAGAACCTGAAGATCATCTTCTATGCCGGCGCCG
CCCTGCCCCAGAACTTGTGGGAGAAGATGGAGCAGCTGTCCATCCGCACCCTGGGCCACAAGGTGCGCATGGTGTCGTCC
TGGGGCTCGACCGAGACCTCGCCCATGATCACCACCGTGCATTTCGACATCCCCCGCGCCGGTATCATCGGCCTGCCCGC
CCCCGGCTGCGAGGTCAAGATGATCCCCAACGGCGGCAAGCTGGAGATGCGCATCAAGGGCCCCAACATCACCCCGGGCT
ACTTCAAGCGCGCCGACCTGACCGCCAAGGCCTTCGACGAGGACGGCTGGTACATGATCGGCGATGCCGGCAAGCTGGCC
GATCCCGACGATCCCTCCAAGGGCATCGTCTTCGACGGCCGCGTGGCCGAGGACTTCAAGCTGACCTCGGGCACCTGGGT
ACATGTGGGAGCGCTGCGCCTGTCGGTGATCGACGCCGCCGCGCCGGTGCTGCAGGACGCGGTGATCACCGGTCACGACC
GTGAATCCGTGGGCCTGCTGGGCTTCGCCAGCCTGCCCGGCTGTTTGCGCCTGTGCCCCGACGCCGCGCCGGACACGCCC
TTGAAGGAACTGATGAAGCGGCCCGAGGTGCGCGACAAGCTGCTGGGCGCCATGAAGGCGCTGGCCGCCGAAGGCAAGGG
CACCTCGCACCGCATCACCCGCGCCCTGCTGATGGACGCCCCGCCCTCCATCGACGCCAACGAGATCACCGACAAGGGCT
ATATCAACCAGCGGGCCGTGCTGACCAATCGCGTCCATCTGGTGGAGAAGCTCTACGCTCCCGGCGATGATCCCGACGTG
ATCCGGATGGGGTGA

Upstream 100 bases:

>100_bases
GTGGAGCAGAACGTGTCGGCCTCGCTGAAGCTGGCCGACCGGGGCTATGTGCTGGAAAACGGCCGCGTGGTGCTTTCGGG
CACCGGCAGCGGCCTTCTGA

Downstream 100 bases:

>100_bases
GCGACCGATAAATGTCATCCCGACGCCCATCGGGGAGGAGGGATCTCGTTCTGACCCGGCTTTGCCGATTCGGCACCAGC
CGGACAAACAGAGATCCCTC

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MTTGSVRPISACRRPRREDKKSGPLKAAREVTMNSVAWAPINFAPARVEVEKLEDGAFIIRSPDSLKPYSRCLGDLLVHW
AERIPNQTFLAQRDGDGWRRVTWAETLKKVEAIGQSLLDRGLNKNRPVMILSDNSIDHALLALAAMHVSVPVSPISPAYS
LISQDHSKLRYIADLLKPGLVYASDGARFADALVIPELARVEVVTSTNPIACGVTFDTLLAATPGPEVENAFANVRPTTV
AKILFTSGSTDMPKGVINTQKMLCSNQQAIRQAWPFLLESHPVLVDWLPWNHTFGGNHNFNMMLRHGGTLYIDEGKPMPG
LVEKTAANLKEVSPTLYFNVPRGFDMLIPMLEKDDELRNSFFKNLKIIFYAGAALPQNLWEKMEQLSIRTLGHKVRMVSS
WGSTETSPMITTVHFDIPRAGIIGLPAPGCEVKMIPNGGKLEMRIKGPNITPGYFKRADLTAKAFDEDGWYMIGDAGKLA
DPDDPSKGIVFDGRVAEDFKLTSGTWVHVGALRLSVIDAAAPVLQDAVITGHDRESVGLLGFASLPGCLRLCPDAAPDTP
LKELMKRPEVRDKLLGAMKALAAEGKGTSHRITRALLMDAPPSIDANEITDKGYINQRAVLTNRVHLVEKLYAPGDDPDV
IRMG

Sequences:

>Translated_644_residues
MTTGSVRPISACRRPRREDKKSGPLKAAREVTMNSVAWAPINFAPARVEVEKLEDGAFIIRSPDSLKPYSRCLGDLLVHW
AERIPNQTFLAQRDGDGWRRVTWAETLKKVEAIGQSLLDRGLNKNRPVMILSDNSIDHALLALAAMHVSVPVSPISPAYS
LISQDHSKLRYIADLLKPGLVYASDGARFADALVIPELARVEVVTSTNPIACGVTFDTLLAATPGPEVENAFANVRPTTV
AKILFTSGSTDMPKGVINTQKMLCSNQQAIRQAWPFLLESHPVLVDWLPWNHTFGGNHNFNMMLRHGGTLYIDEGKPMPG
LVEKTAANLKEVSPTLYFNVPRGFDMLIPMLEKDDELRNSFFKNLKIIFYAGAALPQNLWEKMEQLSIRTLGHKVRMVSS
WGSTETSPMITTVHFDIPRAGIIGLPAPGCEVKMIPNGGKLEMRIKGPNITPGYFKRADLTAKAFDEDGWYMIGDAGKLA
DPDDPSKGIVFDGRVAEDFKLTSGTWVHVGALRLSVIDAAAPVLQDAVITGHDRESVGLLGFASLPGCLRLCPDAAPDTP
LKELMKRPEVRDKLLGAMKALAAEGKGTSHRITRALLMDAPPSIDANEITDKGYINQRAVLTNRVHLVEKLYAPGDDPDV
IRMG
>Mature_643_residues
TTGSVRPISACRRPRREDKKSGPLKAAREVTMNSVAWAPINFAPARVEVEKLEDGAFIIRSPDSLKPYSRCLGDLLVHWA
ERIPNQTFLAQRDGDGWRRVTWAETLKKVEAIGQSLLDRGLNKNRPVMILSDNSIDHALLALAAMHVSVPVSPISPAYSL
ISQDHSKLRYIADLLKPGLVYASDGARFADALVIPELARVEVVTSTNPIACGVTFDTLLAATPGPEVENAFANVRPTTVA
KILFTSGSTDMPKGVINTQKMLCSNQQAIRQAWPFLLESHPVLVDWLPWNHTFGGNHNFNMMLRHGGTLYIDEGKPMPGL
VEKTAANLKEVSPTLYFNVPRGFDMLIPMLEKDDELRNSFFKNLKIIFYAGAALPQNLWEKMEQLSIRTLGHKVRMVSSW
GSTETSPMITTVHFDIPRAGIIGLPAPGCEVKMIPNGGKLEMRIKGPNITPGYFKRADLTAKAFDEDGWYMIGDAGKLAD
PDDPSKGIVFDGRVAEDFKLTSGTWVHVGALRLSVIDAAAPVLQDAVITGHDRESVGLLGFASLPGCLRLCPDAAPDTPL
KELMKRPEVRDKLLGAMKALAAEGKGTSHRITRALLMDAPPSIDANEITDKGYINQRAVLTNRVHLVEKLYAPGDDPDVI
RMG

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807491, Length=554, Percent_Identity=22.0216606498195, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI187761345, Length=324, Percent_Identity=23.7654320987654, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI187761343, Length=324, Percent_Identity=23.7654320987654, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788107, Length=249, Percent_Identity=26.9076305220884, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693145, Length=311, Percent_Identity=26.3665594855305, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=439, Percent_Identity=25.0569476082005, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556552, Length=339, Percent_Identity=24.188790560472, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=451, Percent_Identity=24.6119733924612, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17564090, Length=454, Percent_Identity=23.7885462555066, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 70611; Mature: 70480

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGSVRPISACRRPRREDKKSGPLKAAREVTMNSVAWAPINFAPARVEVEKLEDGAFII
CCCCCCCHHHHHCCCCHHCCCCCCHHHHHHHHHHCEEECCCCCCCCEEEEEEECCCEEEE
RSPDSLKPYSRCLGDLLVHWAERIPNQTFLAQRDGDGWRRVTWAETLKKVEAIGQSLLDR
ECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHC
GLNKNRPVMILSDNSIDHALLALAAMHVSVPVSPISPAYSLISQDHSKLRYIADLLKPGL
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
VYASDGARFADALVIPELARVEVVTSTNPIACGVTFDTLLAATPGPEVENAFANVRPTTV
EEECCCCHHHHHHHCCCCEEEEEEECCCCEEEEEEHHHHHCCCCCCCHHHHHCCCCCCEE
AKILFTSGSTDMPKGVINTQKMLCSNQQAIRQAWPFLLESHPVLVDWLPWNHTFGGNHNF
EEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
NMMLRHGGTLYIDEGKPMPGLVEKTAANLKEVSPTLYFNVPRGFDMLIPMLEKDDELRNS
EEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHHHHH
FFKNLKIIFYAGAALPQNLWEKMEQLSIRTLGHKVRMVSSWGSTETSPMITTVHFDIPRA
HHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCC
GIIGLPAPGCEVKMIPNGGKLEMRIKGPNITPGYFKRADLTAKAFDEDGWYMIGDAGKLA
CEEECCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCC
DPDDPSKGIVFDGRVAEDFKLTSGTWVHVGALRLSVIDAAAPVLQDAVITGHDRESVGLL
CCCCCCCCEEECCCCCCCEEECCCCEEEEHHEEHHHHHHHHHHHHHHHCCCCCCCCCCEE
GFASLPGCLRLCPDAAPDTPLKELMKRPEVRDKLLGAMKALAAEGKGTSHRITRALLMDA
EECCCCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PPSIDANEITDKGYINQRAVLTNRVHLVEKLYAPGDDPDVIRMG
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
TTGSVRPISACRRPRREDKKSGPLKAAREVTMNSVAWAPINFAPARVEVEKLEDGAFII
CCCCCCHHHHHCCCCHHCCCCCCHHHHHHHHHHCEEECCCCCCCCEEEEEEECCCEEEE
RSPDSLKPYSRCLGDLLVHWAERIPNQTFLAQRDGDGWRRVTWAETLKKVEAIGQSLLDR
ECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHC
GLNKNRPVMILSDNSIDHALLALAAMHVSVPVSPISPAYSLISQDHSKLRYIADLLKPGL
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
VYASDGARFADALVIPELARVEVVTSTNPIACGVTFDTLLAATPGPEVENAFANVRPTTV
EEECCCCHHHHHHHCCCCEEEEEEECCCCEEEEEEHHHHHCCCCCCCHHHHHCCCCCCEE
AKILFTSGSTDMPKGVINTQKMLCSNQQAIRQAWPFLLESHPVLVDWLPWNHTFGGNHNF
EEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
NMMLRHGGTLYIDEGKPMPGLVEKTAANLKEVSPTLYFNVPRGFDMLIPMLEKDDELRNS
EEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHHHHH
FFKNLKIIFYAGAALPQNLWEKMEQLSIRTLGHKVRMVSSWGSTETSPMITTVHFDIPRA
HHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCC
GIIGLPAPGCEVKMIPNGGKLEMRIKGPNITPGYFKRADLTAKAFDEDGWYMIGDAGKLA
CEEECCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCC
DPDDPSKGIVFDGRVAEDFKLTSGTWVHVGALRLSVIDAAAPVLQDAVITGHDRESVGLL
CCCCCCCCEEECCCCCCCEEECCCCEEEEHHEEHHHHHHHHHHHHHHHCCCCCCCCCCEE
GFASLPGCLRLCPDAAPDTPLKELMKRPEVRDKLLGAMKALAAEGKGTSHRITRALLMDA
EECCCCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PPSIDANEITDKGYINQRAVLTNRVHLVEKLYAPGDDPDVIRMG
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]