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The map label for this gene is hypA [H]

Identifier: 83310742

GI number: 83310742

Start: 1781449

End: 1781784

Strand: Reverse

Name: hypA [H]

Synonym: amb1643

Alternate gene names: 83310742

Gene position: 1781784-1781449 (Counterclockwise)

Preceding gene: 83310743

Following gene: 83310741

Centisome position: 35.87

GC content: 62.8

Gene sequence:

>336_bases
ATGAGCCTGACCGAAGGCGTAGTGCGCATCCTGGAGGATCAGGCGGCCGAGCACGGCTTCACCCGGGTCAAGACCGTGTG
GCTGGAGATCGGCGAATTGTCCACCGTGGTGCCGGAAAGCATGGAATTCTGCTTTGATGCCGTGGCGCGGGGCAATCCGC
TGACCGAGGGCGCCAAACTGGAAATCATCCGGGTTCCCGGCACCGCCTGGTGCATGGATTGCTCGAAATCGGTCCATGTC
ACCTCGCGGGTGGACCTCTGCCCCGATTGCGGTGGCGCCAAGCTGGCGGTGACCACGGGCGAGGAAATGCGGATCAAGGA
AATGGAGGTGGAGTGA

Upstream 100 bases:

>100_bases
GGAATGTCCGGCCCAGACCCGGAAGGGCGGGCCGCATTGCTGGCCGCCAGTCTCGACCCCTGCGTTCCCTTCCGCATCGA
GGTTGTCCCCCATGCATGAG

Downstream 100 bases:

>100_bases
TGTGCACGGTTTGCGGCTGCGGCGACGCCAAGGTGGACGGCCACGCTCATTCCCCCCATTCCCATGACCACGACCATGAG
CACCATCATGGCCATAGCCA

Product: hydrogenase nickel incorporation protein hypA

Products: NA

Alternate protein names: Protein hupA [H]

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MSLTEGVVRILEDQAAEHGFTRVKTVWLEIGELSTVVPESMEFCFDAVARGNPLTEGAKLEIIRVPGTAWCMDCSKSVHV
TSRVDLCPDCGGAKLAVTTGEEMRIKEMEVE

Sequences:

>Translated_111_residues
MSLTEGVVRILEDQAAEHGFTRVKTVWLEIGELSTVVPESMEFCFDAVARGNPLTEGAKLEIIRVPGTAWCMDCSKSVHV
TSRVDLCPDCGGAKLAVTTGEEMRIKEMEVE
>Mature_110_residues
SLTEGVVRILEDQAAEHGFTRVKTVWLEIGELSTVVPESMEFCFDAVARGNPLTEGAKLEIIRVPGTAWCMDCSKSVHVT
SRVDLCPDCGGAKLAVTTGEEMRIKEMEVE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step [H]

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family [H]

Homologues:

Organism=Escherichia coli, GI1789365, Length=111, Percent_Identity=40.5405405405405, Blast_Score=90, Evalue=3e-20,
Organism=Escherichia coli, GI1789081, Length=112, Percent_Identity=37.5, Blast_Score=82, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000688
- InterPro:   IPR020538 [H]

Pfam domain/function: PF01155 HypA [H]

EC number: NA

Molecular weight: Translated: 12144; Mature: 12013

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTEGVVRILEDQAAEHGFTRVKTVWLEIGELSTVVPESMEFCFDAVARGNPLTEGAKL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHCCCCCCCCCEE
EIIRVPGTAWCMDCSKSVHVTSRVDLCPDCGGAKLAVTTGEEMRIKEMEVE
EEEECCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCC
>Mature Secondary Structure 
SLTEGVVRILEDQAAEHGFTRVKTVWLEIGELSTVVPESMEFCFDAVARGNPLTEGAKL
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHCCCCCCCCCEE
EIIRVPGTAWCMDCSKSVHVTSRVDLCPDCGGAKLAVTTGEEMRIKEMEVE
EEEECCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486288 [H]