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The map label for this gene is map [H]

Identifier: 83310702

GI number: 83310702

Start: 1730087

End: 1730890

Strand: Reverse

Name: map [H]

Synonym: amb1603

Alternate gene names: 83310702

Gene position: 1730890-1730087 (Counterclockwise)

Preceding gene: 83310703

Following gene: 83310701

Centisome position: 34.85

GC content: 63.31

Gene sequence:

>804_bases
ATGGAACACGAAGACAGGGAATTTTCCCGCATCCGCCGCCACGGCCCCGCCGATTTCGACGGCATGCGCAAAGCCGGCCA
GCTGGCCGCCGAGACATTGGACTTCATCGCCCCCCATGTGGTGCCCGGCGTGACCACCGCCGAGCTCGACCGCCTGTGCG
CCGAGTTCATCATCACCCGCGGCGGCATCAATGCGCCCATGAATTACCGGGGCTTTCCCAAATCCATCTGCACCTCGGTC
AATCACGTGGTCTGCCACGGCATTCCGGGGGAAAAGCGCCTGGAGGACGGCGACATCGTCAATATCGACGTGACGCCCAT
CGTCGACGGCTGGCACGGCGATTCGTCGCGTATGTATTACGTCGGCAAGGTCGGCGTGAAGGCCCGCAAGCTGGTGGAGG
TGACCTACGAATCGCTGATGCGCGGCATCGAGGTGGTCAAGCCCGGCGCCACCCTGGGCGATATCGGCTATGCCATCCAG
AGCTTTGCCGAAAAGCACCGCTTTTCCGTGGTGCGCGACTTCTGCGGCCACGGCCTGGGCCGGATCTTCCACGAGCCGCC
CAACGTCATGCATTACGGCAACAAGGGCGAAGGCGGCGTCCTGGCCGAGGGAATGTTCTTCACCATCGAGCCCATGATCA
ATGCTGGCCGCTTTGAGACCAAGATCCTGGCCGACGGCTGGACGGCGGTCACCAAGGACAAATCCCTGTCGGCCCAGTTC
GAGCATTCCATCGGCGTCACCGCCACGGGCTGCGAGATATTTACCCTCTCCCCCGCCGGACGGCACTGCCCTCCATACCT
CTAG

Upstream 100 bases:

>100_bases
GGCGTTGAAGCGATCTGCTATACCTGCCGTCTCGCGCCCGACGGCATCGATCTGGGCGCCCCCTTGACCATGGATTTGAC
TGAGCGGATCTGAAGGACCG

Downstream 100 bases:

>100_bases
GGTAAGCACTTTCCCCTACCACCTTTCGGCCATGGCTTTTGCCGGATGGCGGATAATAGCCGTTTCACGCCCGCCCCCCC
GGGTCGAGACCCTATGCTTT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MEHEDREFSRIRRHGPADFDGMRKAGQLAAETLDFIAPHVVPGVTTAELDRLCAEFIITRGGINAPMNYRGFPKSICTSV
NHVVCHGIPGEKRLEDGDIVNIDVTPIVDGWHGDSSRMYYVGKVGVKARKLVEVTYESLMRGIEVVKPGATLGDIGYAIQ
SFAEKHRFSVVRDFCGHGLGRIFHEPPNVMHYGNKGEGGVLAEGMFFTIEPMINAGRFETKILADGWTAVTKDKSLSAQF
EHSIGVTATGCEIFTLSPAGRHCPPYL

Sequences:

>Translated_267_residues
MEHEDREFSRIRRHGPADFDGMRKAGQLAAETLDFIAPHVVPGVTTAELDRLCAEFIITRGGINAPMNYRGFPKSICTSV
NHVVCHGIPGEKRLEDGDIVNIDVTPIVDGWHGDSSRMYYVGKVGVKARKLVEVTYESLMRGIEVVKPGATLGDIGYAIQ
SFAEKHRFSVVRDFCGHGLGRIFHEPPNVMHYGNKGEGGVLAEGMFFTIEPMINAGRFETKILADGWTAVTKDKSLSAQF
EHSIGVTATGCEIFTLSPAGRHCPPYL
>Mature_267_residues
MEHEDREFSRIRRHGPADFDGMRKAGQLAAETLDFIAPHVVPGVTTAELDRLCAEFIITRGGINAPMNYRGFPKSICTSV
NHVVCHGIPGEKRLEDGDIVNIDVTPIVDGWHGDSSRMYYVGKVGVKARKLVEVTYESLMRGIEVVKPGATLGDIGYAIQ
SFAEKHRFSVVRDFCGHGLGRIFHEPPNVMHYGNKGEGGVLAEGMFFTIEPMINAGRFETKILADGWTAVTKDKSLSAQF
EHSIGVTATGCEIFTLSPAGRHCPPYL

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=247, Percent_Identity=49.3927125506073, Blast_Score=248, Evalue=3e-66,
Organism=Homo sapiens, GI40385867, Length=240, Percent_Identity=42.0833333333333, Blast_Score=193, Evalue=1e-49,
Organism=Escherichia coli, GI1786364, Length=243, Percent_Identity=55.5555555555556, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71996291, Length=243, Percent_Identity=48.559670781893, Blast_Score=230, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6323273, Length=255, Percent_Identity=43.921568627451, Blast_Score=227, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21355531, Length=247, Percent_Identity=50.2024291497976, Blast_Score=243, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24583427, Length=237, Percent_Identity=43.4599156118143, Blast_Score=207, Evalue=7e-54,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 29304; Mature: 29304

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHEDREFSRIRRHGPADFDGMRKAGQLAAETLDFIAPHVVPGVTTAELDRLCAEFIITR
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GGINAPMNYRGFPKSICTSVNHVVCHGIPGEKRLEDGDIVNIDVTPIVDGWHGDSSRMYY
CCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEE
VGKVGVKARKLVEVTYESLMRGIEVVKPGATLGDIGYAIQSFAEKHRFSVVRDFCGHGLG
EECCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RIFHEPPNVMHYGNKGEGGVLAEGMFFTIEPMINAGRFETKILADGWTAVTKDKSLSAQF
HHHCCCCCEEECCCCCCCCEEECCEEEEEECCCCCCCCEEEEEECCCEEECCCCCCCHHH
EHSIGVTATGCEIFTLSPAGRHCPPYL
HHCCCEEEECEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MEHEDREFSRIRRHGPADFDGMRKAGQLAAETLDFIAPHVVPGVTTAELDRLCAEFIITR
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GGINAPMNYRGFPKSICTSVNHVVCHGIPGEKRLEDGDIVNIDVTPIVDGWHGDSSRMYY
CCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEE
VGKVGVKARKLVEVTYESLMRGIEVVKPGATLGDIGYAIQSFAEKHRFSVVRDFCGHGLG
EECCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RIFHEPPNVMHYGNKGEGGVLAEGMFFTIEPMINAGRFETKILADGWTAVTKDKSLSAQF
HHHCCCCCEEECCCCCCCCEEECCEEEEEECCCCCCCCEEEEEECCCEEECCCCCCCHHH
EHSIGVTATGCEIFTLSPAGRHCPPYL
HHCCCEEEECEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]