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The map label for this gene is cspA [C]

Identifier: 83310692

GI number: 83310692

Start: 1721879

End: 1722088

Strand: Reverse

Name: cspA [C]

Synonym: amb1593

Alternate gene names: 83310692

Gene position: 1722088-1721879 (Counterclockwise)

Preceding gene: 83310693

Following gene: 83310691

Centisome position: 34.67

GC content: 59.52

Gene sequence:

>210_bases
ATGCCGAACGGCACCGTTAAGTGGTTCAACGCTACCAAGGGTTATGGCTTTATCCAGCCCGAGGATGGCTCGGCCGACAT
CTTCGTCCACATCTCTGCCGTCGAGCGCGCTGGCATCGGCAACCTGAAGGAAGGCCAGAAGCTCTCCTTCGAGACCGAGC
GCGATCCCCGCAAGGGTAAGAGCTCCGCGGTCAATCTCAAGGCCCTCTAA

Upstream 100 bases:

>100_bases
GCAAGGGAATCCGGCGCCGCTTCAGGTGTCATCGCCGGTCGAACGATTGAACCCCTCGCATGAGAGCATGCGGGGTACTA
CTAGTTATTTGGAGGTTCTC

Downstream 100 bases:

>100_bases
GGCTTTCGAGACTGCGACGCGAAGGGGCGCGGCCTTGTGGCCCGCGCCCTTTTTCGTGCCGATTGCCCAAGCTTCCTTCC
AGGTTCATAGTGAACCCATC

Product: cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MPNGTVKWFNATKGYGFIQPEDGSADIFVHISAVERAGIGNLKEGQKLSFETERDPRKGKSSAVNLKAL

Sequences:

>Translated_69_residues
MPNGTVKWFNATKGYGFIQPEDGSADIFVHISAVERAGIGNLKEGQKLSFETERDPRKGKSSAVNLKAL
>Mature_68_residues
PNGTVKWFNATKGYGFIQPEDGSADIFVHISAVERAGIGNLKEGQKLSFETERDPRKGKSSAVNLKAL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=50, Percent_Identity=62, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=51.5151515151515, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=53.030303030303, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=45.3125, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=58, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI2367114, Length=54, Percent_Identity=51.8518518518518, Blast_Score=65, Evalue=7e-13,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=46.969696969697, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7509; Mature: 7377

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGTVKWFNATKGYGFIQPEDGSADIFVHISAVERAGIGNLKEGQKLSFETERDPRKGK
CCCCEEEEEECCCCCEEECCCCCCEEEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCC
SSAVNLKAL
CCEEEEEEC
>Mature Secondary Structure 
PNGTVKWFNATKGYGFIQPEDGSADIFVHISAVERAGIGNLKEGQKLSFETERDPRKGK
CCCEEEEEECCCCCEEECCCCCCEEEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCC
SSAVNLKAL
CCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]