Click here to switch to the map view.

The map label for this gene is fabI1 [H]

Identifier: 83310690

GI number: 83310690

Start: 1720411

End: 1721295

Strand: Reverse

Name: fabI1 [H]

Synonym: amb1591

Alternate gene names: 83310690

Gene position: 1721295-1720411 (Counterclockwise)

Preceding gene: 83310691

Following gene: 83310689

Centisome position: 34.65

GC content: 66.1

Gene sequence:

>885_bases
GTGTATGTAGGGGAGCCGTGGCCCGTTTCGGCGCCACCCATCCGCTCACCACGAAACGAGGACCGCATGACCGCTCCCGC
TCCCCTGATGGCCGGCAAGAAGGGCCTTGTCATGGGCGTCGCCAACGATCGCTCCATCGCCTGGGGCATCGCGCAGGCTG
CCCGCGCCCAGGGTGCCGAACTGGCCTTCACCTATCAGGGCGAGGCCTTGGAAAAGCGGGTCCGCCCCCTGGCCCAGAGC
GTCGGCTCGGACATCGTGCTGCCCTGCGACGTGTCGGACGAGGCCTCCATCGACGCCGTCTTCGCCGAGTTGGAAAAGCG
CTGGGGCAAGCTGGACTTCGTGGTCCATGCCATCGGCTATTCCGACAAGAACGAGCTGCGCGGCCGCTATGCCGACACGT
CCTTGCAGAACTTCCTGACCTCCATGCACATCTCGGTGTTCTCGTTCACCTCGGTGGCGCGCCGGGCCTCGGCCATGATG
CCCGACGGCGGCTCGCTGCTGACGCTGACCTATTACGGCGCCGAGCGCGTGATGCCCCATTACAACGTCATGGGCGTCGC
CAAGGCGGCTCTCGAAGCCTCGGTGCGCTATCTCGCCGTCGATCTCGGCGGCCAGGGCATCCGCGTCAACTCGCTGTCGG
CCGGTCCCATCAAGACTCTGGCGGCGTCCGGCATCGGCGACTTCCGCTACATCCTGAAGTGGAACGAGTACAACAGCCCG
CTCAAGCGCAACGTCACCCTGGAAGACATCGGCGGCGCCGGCCTTTACCTGCTGTCCGGCCTGTCGTCGGGCGTGTCGGG
CGAGACCCATCATGTGGATTGCGGCTATCACGTGGTCGGCATGAAGGCGGTGGACGCCCCCGACATCACCGTCGCCAAGG
ACTGA

Upstream 100 bases:

>100_bases
GACGCGCTGGGCTCGGAACTCAGCGCCTTCGAGCGCGAGAAGGCGAAGAAATCTGAACTGGGCGAAATTCTGGGATAGCC
TTTGCCACTTGGCCTGTTTC

Downstream 100 bases:

>100_bases
CTCCAACGTTCCAGTCATCCCGAGCGAAGTCGAGGGATCTCCGCCTGCTCCGGCAGGGCCAGTTCTGAAAGGCTGCGCCA
GGACGAGATCCCTCCTTCCG

Product: enoyl-acyl-carrier-protein reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MYVGEPWPVSAPPIRSPRNEDRMTAPAPLMAGKKGLVMGVANDRSIAWGIAQAARAQGAELAFTYQGEALEKRVRPLAQS
VGSDIVLPCDVSDEASIDAVFAELEKRWGKLDFVVHAIGYSDKNELRGRYADTSLQNFLTSMHISVFSFTSVARRASAMM
PDGGSLLTLTYYGAERVMPHYNVMGVAKAALEASVRYLAVDLGGQGIRVNSLSAGPIKTLAASGIGDFRYILKWNEYNSP
LKRNVTLEDIGGAGLYLLSGLSSGVSGETHHVDCGYHVVGMKAVDAPDITVAKD

Sequences:

>Translated_294_residues
MYVGEPWPVSAPPIRSPRNEDRMTAPAPLMAGKKGLVMGVANDRSIAWGIAQAARAQGAELAFTYQGEALEKRVRPLAQS
VGSDIVLPCDVSDEASIDAVFAELEKRWGKLDFVVHAIGYSDKNELRGRYADTSLQNFLTSMHISVFSFTSVARRASAMM
PDGGSLLTLTYYGAERVMPHYNVMGVAKAALEASVRYLAVDLGGQGIRVNSLSAGPIKTLAASGIGDFRYILKWNEYNSP
LKRNVTLEDIGGAGLYLLSGLSSGVSGETHHVDCGYHVVGMKAVDAPDITVAKD
>Mature_294_residues
MYVGEPWPVSAPPIRSPRNEDRMTAPAPLMAGKKGLVMGVANDRSIAWGIAQAARAQGAELAFTYQGEALEKRVRPLAQS
VGSDIVLPCDVSDEASIDAVFAELEKRWGKLDFVVHAIGYSDKNELRGRYADTSLQNFLTSMHISVFSFTSVARRASAMM
PDGGSLLTLTYYGAERVMPHYNVMGVAKAALEASVRYLAVDLGGQGIRVNSLSAGPIKTLAASGIGDFRYILKWNEYNSP
LKRNVTLEDIGGAGLYLLSGLSSGVSGETHHVDCGYHVVGMKAVDAPDITVAKD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=50.7751937984496, Blast_Score=264, Evalue=4e-72,
Organism=Escherichia coli, GI1789378, Length=292, Percent_Identity=29.1095890410959, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=29.5546558704453, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787905, Length=232, Percent_Identity=27.1551724137931, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 31540; Mature: 31540

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVGEPWPVSAPPIRSPRNEDRMTAPAPLMAGKKGLVMGVANDRSIAWGIAQAARAQGAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCE
LAFTYQGEALEKRVRPLAQSVGSDIVLPCDVSDEASIDAVFAELEKRWGKLDFVVHAIGY
EEEEECCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCC
SDKNELRGRYADTSLQNFLTSMHISVFSFTSVARRASAMMPDGGSLLTLTYYGAERVMPH
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHCCCC
YNVMGVAKAALEASVRYLAVDLGGQGIRVNSLSAGPIKTLAASGIGDFRYILKWNEYNSP
CCHHHHHHHHHHHCEEEEEEEECCCEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCC
LKRNVTLEDIGGAGLYLLSGLSSGVSGETHHVDCGYHVVGMKAVDAPDITVAKD
HHCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEECCEEEEEEEEECCCCEEEECC
>Mature Secondary Structure
MYVGEPWPVSAPPIRSPRNEDRMTAPAPLMAGKKGLVMGVANDRSIAWGIAQAARAQGAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCE
LAFTYQGEALEKRVRPLAQSVGSDIVLPCDVSDEASIDAVFAELEKRWGKLDFVVHAIGY
EEEEECCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCC
SDKNELRGRYADTSLQNFLTSMHISVFSFTSVARRASAMMPDGGSLLTLTYYGAERVMPH
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHCCCC
YNVMGVAKAALEASVRYLAVDLGGQGIRVNSLSAGPIKTLAASGIGDFRYILKWNEYNSP
CCHHHHHHHHHHHCEEEEEEEECCCEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCC
LKRNVTLEDIGGAGLYLLSGLSSGVSGETHHVDCGYHVVGMKAVDAPDITVAKD
HHCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEECCEEEEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]