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The map label for this gene is phoB [H]

Identifier: 83310469

GI number: 83310469

Start: 1486809

End: 1487531

Strand: Reverse

Name: phoB [H]

Synonym: amb1370

Alternate gene names: 83310469

Gene position: 1487531-1486809 (Counterclockwise)

Preceding gene: 83310470

Following gene: 83310468

Centisome position: 29.95

GC content: 68.05

Gene sequence:

>723_bases
ATGACCGCCCGCGAGACCGCCAATCCTTCCAAGCCTCTTGTCCTGGTCGTCGAGGATGAAGCGGCGCTGGCCACCATGCT
GCGCTACAACCTGGAAAAGGAAGGCTACCGCGTCGCCGAGGCCGCCGACGGCGAGGAGGCGCTGACCGTGCTGGCCGAGC
GCAAGCCCGATCTGGTGCTGCTGGACTGGATGCTGCCCTCGCTGTCGGGCATCGAGATCTGCCGCCAGATCCGCCGCAAG
CCCGCCACCCGCGAACTGCCCATCATCATGCTGACCGCCCGGGGCGAGGAAGGCGACAAGATCCGAGGCCTGAACACCGG
CGCCGACGACTACCTGACCAAGCCGTTCTCGCTGCCCGAACTGATGGCCCGCGTCCGCGCCCTGCTGCGCCGCGCCCAGC
CGGTCAGCCAGAAGGGACAGATCAGCTGGGGCGACGTCTCCATGGATCTGGCCTCCCACCGGGTGGTGCGGGCCGGCAAG
GCCATCCATCTCGGCCCCACCGAGTTCCGCCTGCTGCAATTCTTCCTGCAGCATCCGGGAACGGTGTTCTCGCGCGAGGA
ACTGCTCGACGCCGTGTGGGGTCCCGACATCTATGTGGAGCCGCGCACGGTGGATGTGCACATCCGCCGCCTGCGCAAGG
CGCTGAACTGCGACACCGAGGCCGACATCATCCGCACCGTGCGGGCCGCCGGCTATGCGCTGGACAACGAGGACGCGGCT
TAG

Upstream 100 bases:

>100_bases
ATCACGCCACCAACATCGCCGAGACCCTGCACTTCCAGATTCTCGGCACCCAGCCCAAGGGCAACCGGCCCAAGGGCGTG
ACCCTCGGAGCCCAGGAATA

Downstream 100 bases:

>100_bases
AGGAAGACTGTCATCCCGAACGGAAGTGAGGGATCTCGTCCTGGCACGGCTTTTCAGAACAGGAACCGGCCATCCTGGCG
GAGATCCCTCGGCTTGGCCT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTARETANPSKPLVLVVEDEAALATMLRYNLEKEGYRVAEAADGEEALTVLAERKPDLVLLDWMLPSLSGIEICRQIRRK
PATRELPIIMLTARGEEGDKIRGLNTGADDYLTKPFSLPELMARVRALLRRAQPVSQKGQISWGDVSMDLASHRVVRAGK
AIHLGPTEFRLLQFFLQHPGTVFSREELLDAVWGPDIYVEPRTVDVHIRRLRKALNCDTEADIIRTVRAAGYALDNEDAA

Sequences:

>Translated_240_residues
MTARETANPSKPLVLVVEDEAALATMLRYNLEKEGYRVAEAADGEEALTVLAERKPDLVLLDWMLPSLSGIEICRQIRRK
PATRELPIIMLTARGEEGDKIRGLNTGADDYLTKPFSLPELMARVRALLRRAQPVSQKGQISWGDVSMDLASHRVVRAGK
AIHLGPTEFRLLQFFLQHPGTVFSREELLDAVWGPDIYVEPRTVDVHIRRLRKALNCDTEADIIRTVRAAGYALDNEDAA
>Mature_239_residues
TARETANPSKPLVLVVEDEAALATMLRYNLEKEGYRVAEAADGEEALTVLAERKPDLVLLDWMLPSLSGIEICRQIRRKP
ATRELPIIMLTARGEEGDKIRGLNTGADDYLTKPFSLPELMARVRALLRRAQPVSQKGQISWGDVSMDLASHRVVRAGKA
IHLGPTEFRLLQFFLQHPGTVFSREELLDAVWGPDIYVEPRTVDVHIRRLRKALNCDTEADIIRTVRAAGYALDNEDAA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=52.0547945205479, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=39.3805309734513, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786911, Length=206, Percent_Identity=42.7184466019417, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=39.5555555555556, Blast_Score=156, Evalue=9e-40,
Organism=Escherichia coli, GI1790860, Length=240, Percent_Identity=40.4166666666667, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=35.5263157894737, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=35.2941176470588, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=36.0869565217391, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=36.2068965517241, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=35.4260089686099, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.117903930131, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=34.841628959276, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=36.1990950226244, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=31.8965517241379, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788191, Length=118, Percent_Identity=31.3559322033898, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI48994928, Length=115, Percent_Identity=36.5217391304348, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790299, Length=141, Percent_Identity=27.6595744680851, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=117, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=129, Percent_Identity=31.0077519379845, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26852; Mature: 26721

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARETANPSKPLVLVVEDEAALATMLRYNLEKEGYRVAEAADGEEALTVLAERKPDLVL
CCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCEEE
LDWMLPSLSGIEICRQIRRKPATRELPIIMLTARGEEGDKIRGLNTGADDYLTKPFSLPE
EECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCHHHHCCCCCHHH
LMARVRALLRRAQPVSQKGQISWGDVSMDLASHRVVRAGKAIHLGPTEFRLLQFFLQHPG
HHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCC
TVFSREELLDAVWGPDIYVEPRTVDVHIRRLRKALNCDTEADIIRTVRAAGYALDNEDAA
CCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TARETANPSKPLVLVVEDEAALATMLRYNLEKEGYRVAEAADGEEALTVLAERKPDLVL
CCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCEEE
LDWMLPSLSGIEICRQIRRKPATRELPIIMLTARGEEGDKIRGLNTGADDYLTKPFSLPE
EECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCHHHHCCCCCHHH
LMARVRALLRRAQPVSQKGQISWGDVSMDLASHRVVRAGKAIHLGPTEFRLLQFFLQHPG
HHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCC
TVFSREELLDAVWGPDIYVEPRTVDVHIRRLRKALNCDTEADIIRTVRAAGYALDNEDAA
CCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]