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The map label for this gene is ycdT [H]

Identifier: 83310444

GI number: 83310444

Start: 1460774

End: 1462270

Strand: Reverse

Name: ycdT [H]

Synonym: amb1345

Alternate gene names: 83310444

Gene position: 1462270-1460774 (Counterclockwise)

Preceding gene: 83310445

Following gene: 83310439

Centisome position: 29.44

GC content: 68.4

Gene sequence:

>1497_bases
GTGAGTCGAAAAGGGGCGAGATCAAGCCGGCGCGTGTCCCAGTCCCCGGCCATCCTCTATTCCGCGCTGTTCGCCATCCT
GGCGGGGGTTGCGGCCTGGCTGGGCGTCGACCTGGCCGAGGGACGGGGCCGCATCGTCGCCGAGCGGACGGTGCTGGCGG
TCCAGAAGAGCCAGTTCATGGCGCAATGGTTCGGGACCACCATCATCTCGGTGGATTACGTCCTGCGCGACGTGATCGGC
CAGACGGGACCGGAAGTGCTGCGCCCTCGCCTCGACCCCGAGCGGGGCCGGCAACTGAACGGCATGCTGGCGGAAAAGAC
CGCCAGCGTGCCGCGGCTGACCGGCATTTCGATCTACAACTCCGACTGCGTCTTCGTGGCGGCGGCCGACACCCGCCTGC
TGGGCTTTCGCAGCAATCAGAAGTCCTGTGCCGAGCGCCCGGAGGCACTGACGGACAAGACCTATATCCAATACGTGCCG
GCGGAGAAATCGGCCAGCCGGCGGCCGGTGATCCTGGTGTCGCGCCATCTGCTGTCCGCCGACGGCAAGCTGCTGGGAGG
CGCCCTGGCGGCCATCGACCTCAGCCATGCCCAGGACTGGATCTCCTCGTTCACCGTCGAGGATCGCGACGTGCTGGCCA
TCGTCGACGGGGACGGCGTTCTGCTGGCCCGCAATCCCGAACTGCCCGCCGTCATCGGAACCCGCACGCCGCAGCCCACC
GGCCAGCCCAGCTTCGGCGAGCAGCGCTCCAGCGCGTCGTTCGTCGCCGTGTCGCCACTGGATGGCCGCGAGCGGATCTT
CGGGCTGAGCAAGCTCGAAAACATTCCACTGATTCTCATGGTCGGCTACGACCTGGCCGGAATCCTGACGGAATGGAGCA
AGCGGGCCGGACAGCTTGCCGCCGGGTTCTTGTTGCTGGTGGCCCTGGCGACCCTTGGCCTGCGCTCCTATCTGACCACC
GTGCGCCAGCGCGAGGAATTGCGCCGGCAGGCGGACACCGATCCATTGACCGGCGTGCCCAACCGCCGGCATCTGATGTC
GGTGGCCGAGCACGAACTGGCCCGCACCTTGCGCTATGGCGGCGCGCTGGCGCTGTTCATGGTGGACATCGACCATTTCA
AGGCCATCAACGACACCTGGGGCCATCCCACCGGCGACCGCGTGATACAGGTGCTTGCCGCCGCCATGGCCGCCTCGGCC
CGCGAGCAGGACACGGCGGGGCGCCTGGGTGGCGAGGAATTCGCCGCCGTGCTGCCCGAGACCGATCTGGAGGGCGCCGC
CATCATGGCCGAGCGGCTGCGGGAAGCGGTCGAGGCCATCACCTCCGCCCTCGATGACCAGGGACAGCCGGTCCGCTTCA
CCGTCAGCATCGGAGTGGCGGCCATCCAGCTCGGCGACGGCTCGGTGGAGGACATTCTCGGCCGTGCCGACCGGGCGCTC
TATGCCGCCAAGCACGGCGGCCGCAATCGGGTGGAGATGGCCGCGACGGCGGAATGA

Upstream 100 bases:

>100_bases
ACGATCCCACCGCGTCAAAGCCACTCCATGCAATTGCCGCGAAGGGTGTGTACACTTCGGCCCTCGCGGTTTTGCGCGAT
CTAAGGGAGAGAATTCCGGA

Downstream 100 bases:

>100_bases
GGGCTGGCGCAAAGCCCCGCTTCGCGGCTGGGCTACCGCCCAGACCTGTTCGGAGGCACAGCCTCCGAGCCACCAGTTCT
TTCATTCCGATAAAATAAAA

Product: response regulator

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSRKGARSSRRVSQSPAILYSALFAILAGVAAWLGVDLAEGRGRIVAERTVLAVQKSQFMAQWFGTTIISVDYVLRDVIG
QTGPEVLRPRLDPERGRQLNGMLAEKTASVPRLTGISIYNSDCVFVAAADTRLLGFRSNQKSCAERPEALTDKTYIQYVP
AEKSASRRPVILVSRHLLSADGKLLGGALAAIDLSHAQDWISSFTVEDRDVLAIVDGDGVLLARNPELPAVIGTRTPQPT
GQPSFGEQRSSASFVAVSPLDGRERIFGLSKLENIPLILMVGYDLAGILTEWSKRAGQLAAGFLLLVALATLGLRSYLTT
VRQREELRRQADTDPLTGVPNRRHLMSVAEHELARTLRYGGALALFMVDIDHFKAINDTWGHPTGDRVIQVLAAAMAASA
REQDTAGRLGGEEFAAVLPETDLEGAAIMAERLREAVEAITSALDDQGQPVRFTVSIGVAAIQLGDGSVEDILGRADRAL
YAAKHGGRNRVEMAATAE

Sequences:

>Translated_498_residues
MSRKGARSSRRVSQSPAILYSALFAILAGVAAWLGVDLAEGRGRIVAERTVLAVQKSQFMAQWFGTTIISVDYVLRDVIG
QTGPEVLRPRLDPERGRQLNGMLAEKTASVPRLTGISIYNSDCVFVAAADTRLLGFRSNQKSCAERPEALTDKTYIQYVP
AEKSASRRPVILVSRHLLSADGKLLGGALAAIDLSHAQDWISSFTVEDRDVLAIVDGDGVLLARNPELPAVIGTRTPQPT
GQPSFGEQRSSASFVAVSPLDGRERIFGLSKLENIPLILMVGYDLAGILTEWSKRAGQLAAGFLLLVALATLGLRSYLTT
VRQREELRRQADTDPLTGVPNRRHLMSVAEHELARTLRYGGALALFMVDIDHFKAINDTWGHPTGDRVIQVLAAAMAASA
REQDTAGRLGGEEFAAVLPETDLEGAAIMAERLREAVEAITSALDDQGQPVRFTVSIGVAAIQLGDGSVEDILGRADRAL
YAAKHGGRNRVEMAATAE
>Mature_497_residues
SRKGARSSRRVSQSPAILYSALFAILAGVAAWLGVDLAEGRGRIVAERTVLAVQKSQFMAQWFGTTIISVDYVLRDVIGQ
TGPEVLRPRLDPERGRQLNGMLAEKTASVPRLTGISIYNSDCVFVAAADTRLLGFRSNQKSCAERPEALTDKTYIQYVPA
EKSASRRPVILVSRHLLSADGKLLGGALAAIDLSHAQDWISSFTVEDRDVLAIVDGDGVLLARNPELPAVIGTRTPQPTG
QPSFGEQRSSASFVAVSPLDGRERIFGLSKLENIPLILMVGYDLAGILTEWSKRAGQLAAGFLLLVALATLGLRSYLTTV
RQREELRRQADTDPLTGVPNRRHLMSVAEHELARTLRYGGALALFMVDIDHFKAINDTWGHPTGDRVIQVLAAAMAASAR
EQDTAGRLGGEEFAAVLPETDLEGAAIMAERLREAVEAITSALDDQGQPVRFTVSIGVAAIQLGDGSVEDILGRADRALY
AAKHGGRNRVEMAATAE

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=220, Percent_Identity=33.6363636363636, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=41.1764705882353, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786584, Length=220, Percent_Identity=35.4545454545455, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=39.1566265060241, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI87082007, Length=204, Percent_Identity=37.2549019607843, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1787816, Length=265, Percent_Identity=31.3207547169811, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=34.7560975609756, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=33.9181286549708, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=34.2857142857143, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081974, Length=188, Percent_Identity=30.8510638297872, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=35.7575757575758, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=28.1609195402299, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=204, Percent_Identity=28.921568627451, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53671; Mature: 53540

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKGARSSRRVSQSPAILYSALFAILAGVAAWLGVDLAEGRGRIVAERTVLAVQKSQFM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEHHHHHHHHHHHHH
AQWFGTTIISVDYVLRDVIGQTGPEVLRPRLDPERGRQLNGMLAEKTASVPRLTGISIYN
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHCCHHCCHHHHHHCCCCCEEEEEEEC
SDCVFVAAADTRLLGFRSNQKSCAERPEALTDKTYIQYVPAEKSASRRPVILVSRHLLSA
CCEEEEEECCCEEEEECCCCHHHHHCCHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHC
DGKLLGGALAAIDLSHAQDWISSFTVEDRDVLAIVDGDGVLLARNPELPAVIGTRTPQPT
CCCHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCEEEECCCCCCC
GQPSFGEQRSSASFVAVSPLDGRERIFGLSKLENIPLILMVGYDLAGILTEWSKRAGQLA
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGFLLLVALATLGLRSYLTTVRQREELRRQADTDPLTGVPNRRHLMSVAEHELARTLRYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GALALFMVDIDHFKAINDTWGHPTGDRVIQVLAAAMAASAREQDTAGRLGGEEFAAVLPE
CEEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHCCC
TDLEGAAIMAERLREAVEAITSALDDQGQPVRFTVSIGVAAIQLGDGSVEDILGRADRAL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEEEEECCCCHHHHHHHHHHHH
YAAKHGGRNRVEMAATAE
HHHHCCCCCCEEEEECCC
>Mature Secondary Structure 
SRKGARSSRRVSQSPAILYSALFAILAGVAAWLGVDLAEGRGRIVAERTVLAVQKSQFM
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEHHHHHHHHHHHHH
AQWFGTTIISVDYVLRDVIGQTGPEVLRPRLDPERGRQLNGMLAEKTASVPRLTGISIYN
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHCCHHCCHHHHHHCCCCCEEEEEEEC
SDCVFVAAADTRLLGFRSNQKSCAERPEALTDKTYIQYVPAEKSASRRPVILVSRHLLSA
CCEEEEEECCCEEEEECCCCHHHHHCCHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHC
DGKLLGGALAAIDLSHAQDWISSFTVEDRDVLAIVDGDGVLLARNPELPAVIGTRTPQPT
CCCHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCEEEECCCCCCC
GQPSFGEQRSSASFVAVSPLDGRERIFGLSKLENIPLILMVGYDLAGILTEWSKRAGQLA
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGFLLLVALATLGLRSYLTTVRQREELRRQADTDPLTGVPNRRHLMSVAEHELARTLRYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GALALFMVDIDHFKAINDTWGHPTGDRVIQVLAAAMAASAREQDTAGRLGGEEFAAVLPE
CEEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHCCC
TDLEGAAIMAERLREAVEAITSALDDQGQPVRFTVSIGVAAIQLGDGSVEDILGRADRAL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEEEEECCCCHHHHHHHHHHHH
YAAKHGGRNRVEMAATAE
HHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]