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The map label for this gene is yafC [C]

Identifier: 83310370

GI number: 83310370

Start: 1373867

End: 1374772

Strand: Reverse

Name: yafC [C]

Synonym: amb1271

Alternate gene names: 83310370

Gene position: 1374772-1373867 (Counterclockwise)

Preceding gene: 83310375

Following gene: 83310369

Centisome position: 27.68

GC content: 69.54

Gene sequence:

>906_bases
ATGGACCGCCTGGACGAGTTGGCCATCTTCATCGCCATCCTGGAGGTGGGCTCCCTGGCCGGGGCGGCCCGGCGGCTGCG
GCGGTCGCCGCCGGCCATCACCCGCTGTCTGGCCGGGCTGGAGGACAGGCTGGGCGTGCGTCTGGTGGAGCGAACGACCC
GCCGACTGGCCCCGACCGACGCCGGACGGCGCTTCGCCGAGCAGGCCCGGCACCTGCTGTCCGGCTATAACGAGGCCTTG
CGCTCCGCCGGTGCCGACGAGCCCCTGCGCGGGCGGCTGCGCGTCACCGCGCCGGTGGTGTTCGGCCGTCGCCACGTGAC
GCCGATCATCGTCGACTTCCTGCGAGCCTATCCGCAAATTTCCATCGAGGCGGTGTTCTCGGACAACAACCTCGACCTGA
TCGAGGAGGAACTGGACGTCGCGGTCCGGATCGGAACGCTGGCTGATTCCACCCTGGTGGCCCGGCGGGTCGGAGAGGTG
GAGAGCGTGCTGGTCGCCAGTCCCGGTTACATTGCCGCCCACGGCCTTCCCCTCGCCGTCGATGAACTGGCGGAGCATGA
CGTGATCTTCACCTCGGGGCGCCCGGTCCTGGTGGAGTGGCGGCTGATCGAGGGCGGCCGCGAGCGGAATGTCCGGCTGA
CGCCGCGCCTGATGGTCAATCAGGTGGAGGCATCCCTGGCCGCCGCCCGCGCCGGCCTGGGAATCGCCCGTGCCCTGTCC
TATCAGGTGGTCGACGACCTGGCGGCTGGGCATCTGGTCCGGGTTCTGCCGGGAAGCGGCGCCCTGCAACCGGTTCATCT
GGTTGTGCCGGGGGGGCGTCACATGGCGGCCCGCACCCGCGCTTTCCTGAATTTCGCCACAAATGGGCTGCAATCCATCG
GCATCGGACACCATCTTTCCTCCTGA

Upstream 100 bases:

>100_bases
TGATCAGCTCCGCGAATGGGGTCTGACACCTTAGCCATTGCCGGACACGGAGAAAAAGCGCCATCCTTGAAAGTCATTAT
TTCAAAATATGGAAGTATAC

Downstream 100 bases:

>100_bases
CAGACGGGCCGGGAACGGCCATCATCTCGCACCGACGACCACCAAGCATCAATCAAGGTTCAGCAATGCCGGTTTCAAAT
TCCCCTGCCCCCAAATGGCT

Product: transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDRLDELAIFIAILEVGSLAGAARRLRRSPPAITRCLAGLEDRLGVRLVERTTRRLAPTDAGRRFAEQARHLLSGYNEAL
RSAGADEPLRGRLRVTAPVVFGRRHVTPIIVDFLRAYPQISIEAVFSDNNLDLIEEELDVAVRIGTLADSTLVARRVGEV
ESVLVASPGYIAAHGLPLAVDELAEHDVIFTSGRPVLVEWRLIEGGRERNVRLTPRLMVNQVEASLAAARAGLGIARALS
YQVVDDLAAGHLVRVLPGSGALQPVHLVVPGGRHMAARTRAFLNFATNGLQSIGIGHHLSS

Sequences:

>Translated_301_residues
MDRLDELAIFIAILEVGSLAGAARRLRRSPPAITRCLAGLEDRLGVRLVERTTRRLAPTDAGRRFAEQARHLLSGYNEAL
RSAGADEPLRGRLRVTAPVVFGRRHVTPIIVDFLRAYPQISIEAVFSDNNLDLIEEELDVAVRIGTLADSTLVARRVGEV
ESVLVASPGYIAAHGLPLAVDELAEHDVIFTSGRPVLVEWRLIEGGRERNVRLTPRLMVNQVEASLAAARAGLGIARALS
YQVVDDLAAGHLVRVLPGSGALQPVHLVVPGGRHMAARTRAFLNFATNGLQSIGIGHHLSS
>Mature_301_residues
MDRLDELAIFIAILEVGSLAGAARRLRRSPPAITRCLAGLEDRLGVRLVERTTRRLAPTDAGRRFAEQARHLLSGYNEAL
RSAGADEPLRGRLRVTAPVVFGRRHVTPIIVDFLRAYPQISIEAVFSDNNLDLIEEELDVAVRIGTLADSTLVARRVGEV
ESVLVASPGYIAAHGLPLAVDELAEHDVIFTSGRPVLVEWRLIEGGRERNVRLTPRLMVNQVEASLAAARAGLGIARALS
YQVVDDLAAGHLVRVLPGSGALQPVHLVVPGGRHMAARTRAFLNFATNGLQSIGIGHHLSS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=32, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789440, Length=254, Percent_Identity=28.3464566929134, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=30.6620209059233, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=30.0341296928328, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789639, Length=301, Percent_Identity=29.2358803986711, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=27.1186440677966, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=31.1284046692607, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788706, Length=255, Percent_Identity=25.0980392156863, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=27.7397260273973, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=296, Percent_Identity=27.3648648648649, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI157672245, Length=179, Percent_Identity=30.7262569832402, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=247, Percent_Identity=25.1012145748988, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789204, Length=301, Percent_Identity=26.578073089701, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=304, Percent_Identity=27.6315789473684, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=32.5, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32553; Mature: 32553

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLDELAIFIAILEVGSLAGAARRLRRSPPAITRCLAGLEDRLGVRLVERTTRRLAPTD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGRRFAEQARHLLSGYNEALRSAGADEPLRGRLRVTAPVVFGRRHVTPIIVDFLRAYPQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCE
SIEAVFSDNNLDLIEEELDVAVRIGTLADSTLVARRVGEVESVLVASPGYIAAHGLPLAV
EEEEEEECCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHEEEECCCEEEECCCCHHH
DELAEHDVIFTSGRPVLVEWRLIEGGRERNVRLTPRLMVNQVEASLAAARAGLGIARALS
HHHHCCCEEEECCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHHHH
YQVVDDLAAGHLVRVLPGSGALQPVHLVVPGGRHMAARTRAFLNFATNGLQSIGIGHHLS
HHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
S
C
>Mature Secondary Structure
MDRLDELAIFIAILEVGSLAGAARRLRRSPPAITRCLAGLEDRLGVRLVERTTRRLAPTD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGRRFAEQARHLLSGYNEALRSAGADEPLRGRLRVTAPVVFGRRHVTPIIVDFLRAYPQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCE
SIEAVFSDNNLDLIEEELDVAVRIGTLADSTLVARRVGEVESVLVASPGYIAAHGLPLAV
EEEEEEECCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHEEEECCCEEEECCCCHHH
DELAEHDVIFTSGRPVLVEWRLIEGGRERNVRLTPRLMVNQVEASLAAARAGLGIARALS
HHHHCCCEEEECCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHHHH
YQVVDDLAAGHLVRVLPGSGALQPVHLVVPGGRHMAARTRAFLNFATNGLQSIGIGHHLS
HHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]