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The map label for this gene is rfbB [H]

Identifier: 83310161

GI number: 83310161

Start: 1124327

End: 1125610

Strand: Reverse

Name: rfbB [H]

Synonym: amb1062

Alternate gene names: 83310161

Gene position: 1125610-1124327 (Counterclockwise)

Preceding gene: 83310162

Following gene: 83310160

Centisome position: 22.66

GC content: 61.06

Gene sequence:

>1284_bases
ATGAGCGCGGCGGATCGGCCTTCCGCCCACCGGGAGGCGGTGTCCTTATCGGTTCGGAATCTTTCAAAATTCTTCAAGAT
CTACCGGAACTCCGGCGACCGCCTGAAGGAATGGTTCCGTCTGGGCAGGTCGCCACGTCATGAAAAATTCTGGGCTTTGC
AGGATGTCAGCCTGACCCTGCGGCAGGGTGAGTGCCTGGGGATCGTTGGCGTCAACGGCTCGGGCAAGAGCACTCTCCTG
AAGATTCTGGCGGGCACGATGGAGGCCTCGGCCGGTGACGTCGAGGTCAACGGGCGCTGCTACGCCCTGCTGGAACTGGG
AACCGGCTTCAATCCCGACGTCAGCGGCCGCGACAATATCACGCTGGCCGCCAGCATGTTGGGCGTCAAGCCGGAATGGG
TGGCTAAACGGGTCGACGATATCATCGAGTTCAGCGGCCTGGGCGAGTTCATCGACCGGCCGCTCAGAACCTATTCGTCT
GGCATGATGGTCAGGCTGGGCTTTTCCATTTTTGCCTTCATCGACCCCGACATCCTGATCATCGATGAGGCCCTGTCGGT
GGGTGACGTCGCCTTCCAGCTGAAATGCATCCGCCGGATGGAAGACCTTATCTATCAAGACCAGCGCAGCGCCATCATCA
TTTCACACGACACCAACGCGCTGGAGCGGTTTTGCGACCGGGTCATTTGGCTTGACCAGGGCCGCGTGCGGATGGAAGGC
GAGCCGTCCGAGGTCGTCAATGCTTATATTATGGCCAATTCCACAGGGGCTCTGTCCGAACACGAACAGGCCATCTCGGT
GCCGGCGCAGACGCCGCCCGGCGTCAGACTCCCGGTCAGCGATGCGGCGGCGCTGTTCCTGTCGGATCAGGCTGAAATCG
TGCAATTGTGGGCGGAAGACAAAGCCGGCGGCTGGATCGTATCGGCGTCTTCCGACCAGGAGTTCACCCTGTGCTACCAG
GCCCGGCTCCATGTGGAATTGACCGGGCCGGTATTCGGTATCAGGATGGTCAATGGTCGTGGCGACGTGGTCGTGTCGAC
CAACACGTTTCTCGAGGGGCTGGACGACGCCACGTTCCATCCCGGAGACGAAGTGATGTTGCGCTGGCCCATCCGAGCAG
GCTTGATGCCTGGGCACTATTTCGTGTCCTGCGGCATTTCCAAGCGGGAAGACCCCCACGACTTCCTGGTCCGCCACCTC
GACGCCTACCATTTCACCATCCAGGGGGAGGCCAGGGGGAGCGGGTTCGTCACCATCAGCGGCAAGCCGGTGATAAGCCG
ATGA

Upstream 100 bases:

>100_bases
CCCGTCATGATCTGACCTTGACTGCCTTGTGGACAATCGTGTTGATCGCATTGGCGACGCTTGCTTGGCGACGGTTGGGC
CGTGACGTGAGAGACCTGCT

Downstream 100 bases:

>100_bases
GCGGGATAACCTCCCGAGATCCGGTCTTCCTCCTGGGGATCCCACGCTCGGGGACAACCCTGCTGGCCCGGCTGCTGACC
AATCACCCCGAGGTCATGGC

Product: ABC-type polysaccharide/polyol phosphate transport system

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAADRPSAHREAVSLSVRNLSKFFKIYRNSGDRLKEWFRLGRSPRHEKFWALQDVSLTLRQGECLGIVGVNGSGKSTLL
KILAGTMEASAGDVEVNGRCYALLELGTGFNPDVSGRDNITLAASMLGVKPEWVAKRVDDIIEFSGLGEFIDRPLRTYSS
GMMVRLGFSIFAFIDPDILIIDEALSVGDVAFQLKCIRRMEDLIYQDQRSAIIISHDTNALERFCDRVIWLDQGRVRMEG
EPSEVVNAYIMANSTGALSEHEQAISVPAQTPPGVRLPVSDAAALFLSDQAEIVQLWAEDKAGGWIVSASSDQEFTLCYQ
ARLHVELTGPVFGIRMVNGRGDVVVSTNTFLEGLDDATFHPGDEVMLRWPIRAGLMPGHYFVSCGISKREDPHDFLVRHL
DAYHFTIQGEARGSGFVTISGKPVISR

Sequences:

>Translated_427_residues
MSAADRPSAHREAVSLSVRNLSKFFKIYRNSGDRLKEWFRLGRSPRHEKFWALQDVSLTLRQGECLGIVGVNGSGKSTLL
KILAGTMEASAGDVEVNGRCYALLELGTGFNPDVSGRDNITLAASMLGVKPEWVAKRVDDIIEFSGLGEFIDRPLRTYSS
GMMVRLGFSIFAFIDPDILIIDEALSVGDVAFQLKCIRRMEDLIYQDQRSAIIISHDTNALERFCDRVIWLDQGRVRMEG
EPSEVVNAYIMANSTGALSEHEQAISVPAQTPPGVRLPVSDAAALFLSDQAEIVQLWAEDKAGGWIVSASSDQEFTLCYQ
ARLHVELTGPVFGIRMVNGRGDVVVSTNTFLEGLDDATFHPGDEVMLRWPIRAGLMPGHYFVSCGISKREDPHDFLVRHL
DAYHFTIQGEARGSGFVTISGKPVISR
>Mature_426_residues
SAADRPSAHREAVSLSVRNLSKFFKIYRNSGDRLKEWFRLGRSPRHEKFWALQDVSLTLRQGECLGIVGVNGSGKSTLLK
ILAGTMEASAGDVEVNGRCYALLELGTGFNPDVSGRDNITLAASMLGVKPEWVAKRVDDIIEFSGLGEFIDRPLRTYSSG
MMVRLGFSIFAFIDPDILIIDEALSVGDVAFQLKCIRRMEDLIYQDQRSAIIISHDTNALERFCDRVIWLDQGRVRMEGE
PSEVVNAYIMANSTGALSEHEQAISVPAQTPPGVRLPVSDAAALFLSDQAEIVQLWAEDKAGGWIVSASSDQEFTLCYQA
RLHVELTGPVFGIRMVNGRGDVVVSTNTFLEGLDDATFHPGDEVMLRWPIRAGLMPGHYFVSCGISKREDPHDFLVRHLD
AYHFTIQGEARGSGFVTISGKPVISR

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI150417984, Length=220, Percent_Identity=25.9090909090909, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI31657092, Length=244, Percent_Identity=27.8688524590164, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI153792144, Length=229, Percent_Identity=24.4541484716157, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI47078218, Length=232, Percent_Identity=26.2931034482759, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI45446740, Length=232, Percent_Identity=26.2931034482759, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI27436953, Length=207, Percent_Identity=23.6714975845411, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI6005701, Length=204, Percent_Identity=27.4509803921569, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI105990541, Length=200, Percent_Identity=27, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI27881501, Length=209, Percent_Identity=25.8373205741627, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI30795238, Length=209, Percent_Identity=25.8373205741627, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI27477115, Length=204, Percent_Identity=24.5098039215686, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI116734710, Length=212, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27262624, Length=207, Percent_Identity=23.6714975845411, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI27262626, Length=207, Percent_Identity=23.6714975845411, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI21536376, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789032, Length=256, Percent_Identity=26.953125, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790467, Length=202, Percent_Identity=28.2178217821782, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787547, Length=254, Percent_Identity=28.3464566929134, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789891, Length=214, Percent_Identity=27.1028037383178, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994943, Length=245, Percent_Identity=28.1632653061224, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081791, Length=256, Percent_Identity=23.828125, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787758, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788761, Length=214, Percent_Identity=24.2990654205607, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081709, Length=203, Percent_Identity=23.6453201970443, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787029, Length=212, Percent_Identity=24.5283018867925, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082267, Length=210, Percent_Identity=25.2380952380952, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081782, Length=214, Percent_Identity=24.7663551401869, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786398, Length=211, Percent_Identity=25.5924170616114, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788897, Length=222, Percent_Identity=25.6756756756757, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788450, Length=204, Percent_Identity=24.5098039215686, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787370, Length=211, Percent_Identity=22.7488151658768, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786803, Length=219, Percent_Identity=22.8310502283105, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787712, Length=208, Percent_Identity=25.4807692307692, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787792, Length=233, Percent_Identity=27.0386266094421, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786703, Length=207, Percent_Identity=28.0193236714976, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788165, Length=205, Percent_Identity=27.8048780487805, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786563, Length=225, Percent_Identity=22.6666666666667, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=217, Percent_Identity=26.2672811059908, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533608, Length=215, Percent_Identity=23.2558139534884, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133930907, Length=202, Percent_Identity=26.7326732673267, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24643674, Length=214, Percent_Identity=24.7663551401869, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221512771, Length=229, Percent_Identity=23.5807860262009, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28573571, Length=221, Percent_Identity=24.8868778280543, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573573, Length=221, Percent_Identity=24.8868778280543, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641565, Length=221, Percent_Identity=28.0542986425339, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221500365, Length=233, Percent_Identity=21.8884120171674, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007184, Length=233, Percent_Identity=21.8884120171674, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 47156; Mature: 47025

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAADRPSAHREAVSLSVRNLSKFFKIYRNSGDRLKEWFRLGRSPRHEKFWALQDVSLTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCEEEE
RQGECLGIVGVNGSGKSTLLKILAGTMEASAGDVEVNGRCYALLELGTGFNPDVSGRDNI
ECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCCCCCCCCCCCE
TLAASMLGVKPEWVAKRVDDIIEFSGLGEFIDRPLRTYSSGMMVRLGFSIFAFIDPDILI
EEEEEHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCEEE
IDEALSVGDVAFQLKCIRRMEDLIYQDQRSAIIISHDTNALERFCDRVIWLDQGRVRMEG
EECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHEEEECCCEEEECC
EPSEVVNAYIMANSTGALSEHEQAISVPAQTPPGVRLPVSDAAALFLSDQAEIVQLWAED
CHHHHEEEEEEECCCCCCCCCHHHEECCCCCCCCCEEECCCCEEEEECCCHHEEEEEECC
KAGGWIVSASSDQEFTLCYQARLHVELTGPVFGIRMVNGRGDVVVSTNTFLEGLDDATFH
CCCCEEEECCCCCCEEEEEEEEEEEEECCCEEEEEEECCCCCEEEECCHHHHCCCCCCCC
PGDEVMLRWPIRAGLMPGHYFVSCGISKREDPHDFLVRHLDAYHFTIQGEARGSGFVTIS
CCCCEEEEECHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEC
GKPVISR
CCCCCCC
>Mature Secondary Structure 
SAADRPSAHREAVSLSVRNLSKFFKIYRNSGDRLKEWFRLGRSPRHEKFWALQDVSLTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCEEEE
RQGECLGIVGVNGSGKSTLLKILAGTMEASAGDVEVNGRCYALLELGTGFNPDVSGRDNI
ECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCCCCCCCCCCCE
TLAASMLGVKPEWVAKRVDDIIEFSGLGEFIDRPLRTYSSGMMVRLGFSIFAFIDPDILI
EEEEEHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCEEE
IDEALSVGDVAFQLKCIRRMEDLIYQDQRSAIIISHDTNALERFCDRVIWLDQGRVRMEG
EECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHEEEECCCEEEECC
EPSEVVNAYIMANSTGALSEHEQAISVPAQTPPGVRLPVSDAAALFLSDQAEIVQLWAED
CHHHHEEEEEEECCCCCCCCCHHHEECCCCCCCCCEEECCCCEEEEECCCHHEEEEEECC
KAGGWIVSASSDQEFTLCYQARLHVELTGPVFGIRMVNGRGDVVVSTNTFLEGLDDATFH
CCCCEEEECCCCCCEEEEEEEEEEEEECCCEEEEEEECCCCCEEEECCHHHHCCCCCCCC
PGDEVMLRWPIRAGLMPGHYFVSCGISKREDPHDFLVRHLDAYHFTIQGEARGSGFVTIS
CCCCEEEEECHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEC
GKPVISR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626291 [H]