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The map label for this gene is cobB [H]

Identifier: 83309966

GI number: 83309966

Start: 927423

End: 928145

Strand: Reverse

Name: cobB [H]

Synonym: amb0867

Alternate gene names: 83309966

Gene position: 928145-927423 (Counterclockwise)

Preceding gene: 83309969

Following gene: 83309965

Centisome position: 18.69

GC content: 67.91

Gene sequence:

>723_bases
ATGCCCTCCCTTCCCGGCAATGGAACCATCGTCGTCCTGACCGGCGCCGGAATTTCCAAGGAATCCGGCCTGGACACCTT
CCGCGACAAGGACGGCATCTGGGCCAAGGTCCGCATGGAGGACGTGGCGACGCCCGACGGCTTTCGCCGCGACCCGGCCA
AGGTGCACGCCTTCTACAATGCGCGGCGGCGCAATCTGGCCGAGGGCGGCGTCGCCCCCAACGCGGCCCACGCCGCCCTG
GCCCGGCTGGAGAGGGAGTGGCCGGGCGAAGTGCTGCTGGTGACCCAGAACATCGACGACCTACACGAGCGGGCCGGGTC
GGAAAACCTGATCCACATGCATGGCGAGCTGCTGAAGATCCGTTGCGTCAAATGCGCCCGGCCCTTCGCCTGGACCCGGG
ACCTGGGGATCGAGGACAAGTGCCCCGATTGCTACCGCGTCGGCTCGCTGCGCCCCCATGTGGTGTGGTTCGGCGAGGTG
CCGCTGCACATGGAGCGCATTTTCGAGGCACTGGCCCAATGCGCCCTGTTCCTGTCCATCGGCACCTCGGGCCATGTCTA
CCCGGCGGCGGGCTTCGTCCAGACGGTGCGAGGGCTGGGCCGCGCCCAGGCGGTCGAGCTTAATCTGGAGCCCTCGGAAG
GGGCGTCGCTGTTCCACGAATCCCGCCTGGGGCCTGCCTCCCGCCTAGTTCCCGCGTTCGTCGAGGATCTGCTGTCACGC
TAA

Upstream 100 bases:

>100_bases
GCCTGATGATAATTTCAAGCTAATGATTGCCGTTACGCGGTCTTGACCACTCAAGTCCGCTCGGGTTCCATGGACCTTTC
CCCAGCGGCAGGAGTCTCCC

Downstream 100 bases:

>100_bases
CCGCTCTTGACTCCTGACGCGATATGAGTAGCCATGTGCCGTCATTTCTAAGGGGGACACCTGATATGACGGCCCATGGC
GGAGACGGAAAGTCCCGCAA

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MPSLPGNGTIVVLTGAGISKESGLDTFRDKDGIWAKVRMEDVATPDGFRRDPAKVHAFYNARRRNLAEGGVAPNAAHAAL
ARLEREWPGEVLLVTQNIDDLHERAGSENLIHMHGELLKIRCVKCARPFAWTRDLGIEDKCPDCYRVGSLRPHVVWFGEV
PLHMERIFEALAQCALFLSIGTSGHVYPAAGFVQTVRGLGRAQAVELNLEPSEGASLFHESRLGPASRLVPAFVEDLLSR

Sequences:

>Translated_240_residues
MPSLPGNGTIVVLTGAGISKESGLDTFRDKDGIWAKVRMEDVATPDGFRRDPAKVHAFYNARRRNLAEGGVAPNAAHAAL
ARLEREWPGEVLLVTQNIDDLHERAGSENLIHMHGELLKIRCVKCARPFAWTRDLGIEDKCPDCYRVGSLRPHVVWFGEV
PLHMERIFEALAQCALFLSIGTSGHVYPAAGFVQTVRGLGRAQAVELNLEPSEGASLFHESRLGPASRLVPAFVEDLLSR
>Mature_239_residues
PSLPGNGTIVVLTGAGISKESGLDTFRDKDGIWAKVRMEDVATPDGFRRDPAKVHAFYNARRRNLAEGGVAPNAAHAALA
RLEREWPGEVLLVTQNIDDLHERAGSENLIHMHGELLKIRCVKCARPFAWTRDLGIEDKCPDCYRVGSLRPHVVWFGEVP
LHMERIFEALAQCALFLSIGTSGHVYPAAGFVQTVRGLGRAQAVELNLEPSEGASLFHESRLGPASRLVPAFVEDLLSR

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=249, Percent_Identity=38.9558232931727, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI300795542, Length=231, Percent_Identity=39.8268398268398, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI13787215, Length=236, Percent_Identity=39.4067796610169, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI7657575, Length=200, Percent_Identity=30, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI6912660, Length=200, Percent_Identity=32, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI63054862, Length=197, Percent_Identity=31.4720812182741, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI308199517, Length=230, Percent_Identity=59.1304347826087, Blast_Score=272, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17541892, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990482, Length=232, Percent_Identity=28.0172413793103, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990487, Length=234, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17567771, Length=221, Percent_Identity=28.5067873303167, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648389, Length=211, Percent_Identity=27.9620853080569, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571445, Length=122, Percent_Identity=37.7049180327869, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17137536, Length=221, Percent_Identity=28.9592760180996, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26353; Mature: 26222

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLPGNGTIVVLTGAGISKESGLDTFRDKDGIWAKVRMEDVATPDGFRRDPAKVHAFYN
CCCCCCCCEEEEEECCCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ARRRNLAEGGVAPNAAHAALARLEREWPGEVLLVTQNIDDLHERAGSENLIHMHGELLKI
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEECCHHHHH
RCVKCARPFAWTRDLGIEDKCPDCYRVGSLRPHVVWFGEVPLHMERIFEALAQCALFLSI
HHHHHCCCCCCHHCCCCCCCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHC
GTSGHVYPAAGFVQTVRGLGRAQAVELNLEPSEGASLFHESRLGPASRLVPAFVEDLLSR
CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSLPGNGTIVVLTGAGISKESGLDTFRDKDGIWAKVRMEDVATPDGFRRDPAKVHAFYN
CCCCCCCEEEEEECCCCCCCCCCCHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ARRRNLAEGGVAPNAAHAALARLEREWPGEVLLVTQNIDDLHERAGSENLIHMHGELLKI
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEECCHHHHH
RCVKCARPFAWTRDLGIEDKCPDCYRVGSLRPHVVWFGEVPLHMERIFEALAQCALFLSI
HHHHHCCCCCCHHCCCCCCCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHC
GTSGHVYPAAGFVQTVRGLGRAQAVELNLEPSEGASLFHESRLGPASRLVPAFVEDLLSR
CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]