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The map label for this gene is hpcB [H]

Identifier: 83309903

GI number: 83309903

Start: 861447

End: 862310

Strand: Reverse

Name: hpcB [H]

Synonym: amb0804

Alternate gene names: 83309903

Gene position: 862310-861447 (Counterclockwise)

Preceding gene: 83309904

Following gene: 83309902

Centisome position: 17.36

GC content: 62.96

Gene sequence:

>864_bases
ATGGGTAATCTGGTTTTCGCCGCCAAGGTGGCTCACGTCCCCACCATGTACCTGTCCGAGCAGGAAGGCCCCTTCAAGGG
CTGCCGCCAGTCCGCCGTGGACGGCCACCGCGCCATGGGCAAGCGCATGAAGGACCTGGGCGTCGACACCGTCATCGTGC
TCGACACCCATTGGCTGGTGAACAACGCCTATCACATCAACGGCAACGCCCATTTCAAGGGCGTGTTCACCTCCAACGAG
TTCCCCCATCTGCTGGCCGACATGCCCTATGAGCACGAGGGCAACCCGGAGATGAGCGACCTGATCGCCAAGATCGCCAC
CGAGGAAAAGGGCGTCACCGTGCGCTCGCACCACATCGCAAGCCTGGAGCTGGAATACGGCACCCTGATTCCCATGTGGC
TGATGGGCGCCAAGGAATACGGCTTCAAGGTGGTGTCGGTGGCCTGCCTGTGCACCGTCCACGACCACATGGAATCCGCC
AAGGTGGGCGAGGCCATCCGCGAGGCCATCCGGCGCATGGACGGCAATTTCGCCATCGTCGCCTCGGGCTCGCTGTCCCA
CGCCATCCATCCCAACACGACGCTGAAGACCATGGATCACTTCTTCACCATCTCCAACGAGTTCTACAAGCAGGTGGACC
TGCGGGCGTTGGACCTGTGGAAGGAAGGGCGCTTCAAGGAATTCACCGACATGCTGCCGCTCTACGCCAAGACCTGCCAC
GGCGAGGGCCTGATGCACGACACCGCCATGCTGCTGGGCGCCCTGGGTTGGGACAAGTACGATCGCGGCGTCGAGATCGT
CACTCCCTATTTCGAGGCGGCCGGAACCGGCCAGGTCAACGCGGTCTTCCCCGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
ATCCCTCCGCCCAAGGGCGTCGGGATGACACGTCGAGGCAAGGGCCGCCAAAACAAACGTGTTCAGGGCAGAGTTTCAAC
GAGAGAGAGGAGCGTCCAAA

Downstream 100 bases:

>100_bases
TTTGCACAACCTGTCATCCCGAGCGCCGGCGAGGGATCTCCGCCTGGAACGGTGAAGCCAACTTGGACACGCCGTTCCAG
GACGAGATCCCTCCTCCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MGNLVFAAKVAHVPTMYLSEQEGPFKGCRQSAVDGHRAMGKRMKDLGVDTVIVLDTHWLVNNAYHINGNAHFKGVFTSNE
FPHLLADMPYEHEGNPEMSDLIAKIATEEKGVTVRSHHIASLELEYGTLIPMWLMGAKEYGFKVVSVACLCTVHDHMESA
KVGEAIREAIRRMDGNFAIVASGSLSHAIHPNTTLKTMDHFFTISNEFYKQVDLRALDLWKEGRFKEFTDMLPLYAKTCH
GEGLMHDTAMLLGALGWDKYDRGVEIVTPYFEAAGTGQVNAVFPVAA

Sequences:

>Translated_287_residues
MGNLVFAAKVAHVPTMYLSEQEGPFKGCRQSAVDGHRAMGKRMKDLGVDTVIVLDTHWLVNNAYHINGNAHFKGVFTSNE
FPHLLADMPYEHEGNPEMSDLIAKIATEEKGVTVRSHHIASLELEYGTLIPMWLMGAKEYGFKVVSVACLCTVHDHMESA
KVGEAIREAIRRMDGNFAIVASGSLSHAIHPNTTLKTMDHFFTISNEFYKQVDLRALDLWKEGRFKEFTDMLPLYAKTCH
GEGLMHDTAMLLGALGWDKYDRGVEIVTPYFEAAGTGQVNAVFPVAA
>Mature_286_residues
GNLVFAAKVAHVPTMYLSEQEGPFKGCRQSAVDGHRAMGKRMKDLGVDTVIVLDTHWLVNNAYHINGNAHFKGVFTSNEF
PHLLADMPYEHEGNPEMSDLIAKIATEEKGVTVRSHHIASLELEYGTLIPMWLMGAKEYGFKVVSVACLCTVHDHMESAK
VGEAIREAIRRMDGNFAIVASGSLSHAIHPNTTLKTMDHFFTISNEFYKQVDLRALDLWKEGRFKEFTDMLPLYAKTCHG
EGLMHDTAMLLGALGWDKYDRGVEIVTPYFEAAGTGQVNAVFPVAA

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 31873; Mature: 31742

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLVFAAKVAHVPTMYLSEQEGPFKGCRQSAVDGHRAMGKRMKDLGVDTVIVLDTHWLV
CCCEEEEEHHHHCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCEEE
NNAYHINGNAHFKGVFTSNEFPHLLADMPYEHEGNPEMSDLIAKIATEEKGVTVRSHHIA
CCEEEECCCEEEEEEECCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECEEE
SLELEYGTLIPMWLMGAKEYGFKVVSVACLCTVHDHMESAKVGEAIREAIRRMDGNFAIV
EEEECCCCCHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ASGSLSHAIHPNTTLKTMDHFFTISNEFYKQVDLRALDLWKEGRFKEFTDMLPLYAKTCH
ECCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
GEGLMHDTAMLLGALGWDKYDRGVEIVTPYFEAAGTGQVNAVFPVAA
CCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCCCEEEEECCC
>Mature Secondary Structure 
GNLVFAAKVAHVPTMYLSEQEGPFKGCRQSAVDGHRAMGKRMKDLGVDTVIVLDTHWLV
CCEEEEEHHHHCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCEEE
NNAYHINGNAHFKGVFTSNEFPHLLADMPYEHEGNPEMSDLIAKIATEEKGVTVRSHHIA
CCEEEECCCEEEEEEECCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECEEE
SLELEYGTLIPMWLMGAKEYGFKVVSVACLCTVHDHMESAKVGEAIREAIRRMDGNFAIV
EEEECCCCCHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ASGSLSHAIHPNTTLKTMDHFFTISNEFYKQVDLRALDLWKEGRFKEFTDMLPLYAKTCH
ECCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
GEGLMHDTAMLLGALGWDKYDRGVEIVTPYFEAAGTGQVNAVFPVAA
CCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]