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The map label for this gene is fcl [C]

Identifier: 83309898

GI number: 83309898

Start: 856810

End: 857721

Strand: Reverse

Name: fcl [C]

Synonym: amb0799

Alternate gene names: 83309898

Gene position: 857721-856810 (Counterclockwise)

Preceding gene: 83309899

Following gene: 83309894

Centisome position: 17.27

GC content: 66.12

Gene sequence:

>912_bases
GTGTTCGTGGCGGGTCATCGCGGCATGGCGGGCAGCGCCATCCTCCGCCGCCTGCAATCCGAGGATTGCGAGACTCTCAC
CGTCGACCGCTTGGCGCTCGATCTTCGCAACCAGTCTGCCGTCGAGGCGTGGATGGAGGACAAACGGCCCGACGCCGTGT
TCCTGGCCGCCGCCCTGGTAGGCGGCATCCGCGCCAATTCCACCCGCCCGGCCGAGTTCCTCTATGACAATCTCGCCGTC
GAGATGAATGTCATCCATGCGGCCCACCGGGTGGGGGTGTCGCGGCTGCTGTTCCTGGGCTCGTCCTGCGCCTATCCCCG
GGAGGCCGCCCAGCCCATGGCCGAGTCCTCCATGCTGACCGGTCCGCTGGAGCCCACCAACGAGGCCTATGCCATCGCCA
AGATCGCCGGCATCAAGCTGTGCGAGGCCTATCACCGCCAGTATGGCCGCCACTTCATGTCGGCGGTGCCGGCCAGCCTG
ATCGGGCCGGGCGACCGCTTCGACGCCGAGAACGGCCATGTGGGCGCCGCCCTGGTCATGAAGTTCCACGACGCCGTCCA
GCGTGGCGCCGATACGGTGGAATTGTGGGGAACGGGAAGCCCGATCCGCGAATTCCTCTACGTGGACGATCTGGCCGACG
CCTGCGTCTTCCTGATGAAGTCCCTGGGCGGCGGCGAGATCATCAATGTGGGCTCGGGCATCGAAGCCTCTATCCGCGAG
TTGGCCGAACTCACCGCCCGCGTGGTGGGGTTCAAGGGCAAGCTGTCCTTCGACACCACCAAACCCGACGGCATGATGCG
CAAGCTGGTGGATTCCACCCGCATCCGCGCCATGGGCTGGCAGGCCGCCACATCGCTGGAGGAGAGCATCCGGCGCGGCT
ATGAGTGGTATCTGGCCAATTCGAAGGCTTAA

Upstream 100 bases:

>100_bases
CTGATGACAGCTCCCGGGGTCCGGCCTATGATCGGCGCCAGTCAGGCAATAGGGTGAGGACACAATGGCCGGGGCGAATT
TTTCTTTGGCGGGCAAGCGG

Downstream 100 bases:

>100_bases
TGCGGCTCCCGACCGTTTCCATCGTCCCGCATGTCAAGACTGGCGCCGGTGATCATGACGTTCTACCCGGTGTGATGACG
TCCGCGGCCTGATCCAGGCT

Product: nucleoside-diphosphate-sugar epimerase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MFVAGHRGMAGSAILRRLQSEDCETLTVDRLALDLRNQSAVEAWMEDKRPDAVFLAAALVGGIRANSTRPAEFLYDNLAV
EMNVIHAAHRVGVSRLLFLGSSCAYPREAAQPMAESSMLTGPLEPTNEAYAIAKIAGIKLCEAYHRQYGRHFMSAVPASL
IGPGDRFDAENGHVGAALVMKFHDAVQRGADTVELWGTGSPIREFLYVDDLADACVFLMKSLGGGEIINVGSGIEASIRE
LAELTARVVGFKGKLSFDTTKPDGMMRKLVDSTRIRAMGWQAATSLEESIRRGYEWYLANSKA

Sequences:

>Translated_303_residues
MFVAGHRGMAGSAILRRLQSEDCETLTVDRLALDLRNQSAVEAWMEDKRPDAVFLAAALVGGIRANSTRPAEFLYDNLAV
EMNVIHAAHRVGVSRLLFLGSSCAYPREAAQPMAESSMLTGPLEPTNEAYAIAKIAGIKLCEAYHRQYGRHFMSAVPASL
IGPGDRFDAENGHVGAALVMKFHDAVQRGADTVELWGTGSPIREFLYVDDLADACVFLMKSLGGGEIINVGSGIEASIRE
LAELTARVVGFKGKLSFDTTKPDGMMRKLVDSTRIRAMGWQAATSLEESIRRGYEWYLANSKA
>Mature_303_residues
MFVAGHRGMAGSAILRRLQSEDCETLTVDRLALDLRNQSAVEAWMEDKRPDAVFLAAALVGGIRANSTRPAEFLYDNLAV
EMNVIHAAHRVGVSRLLFLGSSCAYPREAAQPMAESSMLTGPLEPTNEAYAIAKIAGIKLCEAYHRQYGRHFMSAVPASL
IGPGDRFDAENGHVGAALVMKFHDAVQRGADTVELWGTGSPIREFLYVDDLADACVFLMKSLGGGEIINVGSGIEASIRE
LAELTARVVGFKGKLSFDTTKPDGMMRKLVDSTRIRAMGWQAATSLEESIRRGYEWYLANSKA

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=309, Percent_Identity=31.0679611650485, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI7657641, Length=274, Percent_Identity=24.4525547445255, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI42516563, Length=313, Percent_Identity=25.5591054313099, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788365, Length=313, Percent_Identity=51.4376996805112, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=22.4431818181818, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554428, Length=308, Percent_Identity=28.2467532467532, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19922778, Length=312, Percent_Identity=29.8076923076923, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21356223, Length=314, Percent_Identity=23.2484076433121, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 32972; Mature: 32972

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVAGHRGMAGSAILRRLQSEDCETLTVDRLALDLRNQSAVEAWMEDKRPDAVFLAAALV
CEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHH
GGIRANSTRPAEFLYDNLAVEMNVIHAAHRVGVSRLLFLGSSCAYPREAAQPMAESSMLT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHCCCC
GPLEPTNEAYAIAKIAGIKLCEAYHRQYGRHFMSAVPASLIGPGDRFDAENGHVGAALVM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHH
KFHDAVQRGADTVELWGTGSPIREFLYVDDLADACVFLMKSLGGGEIINVGSGIEASIRE
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHH
LAELTARVVGFKGKLSFDTTKPDGMMRKLVDSTRIRAMGWQAATSLEESIRRGYEWYLAN
HHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SKA
CCC
>Mature Secondary Structure
MFVAGHRGMAGSAILRRLQSEDCETLTVDRLALDLRNQSAVEAWMEDKRPDAVFLAAALV
CEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHH
GGIRANSTRPAEFLYDNLAVEMNVIHAAHRVGVSRLLFLGSSCAYPREAAQPMAESSMLT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHCCCC
GPLEPTNEAYAIAKIAGIKLCEAYHRQYGRHFMSAVPASLIGPGDRFDAENGHVGAALVM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHH
KFHDAVQRGADTVELWGTGSPIREFLYVDDLADACVFLMKSLGGGEIINVGSGIEASIRE
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHH
LAELTARVVGFKGKLSFDTTKPDGMMRKLVDSTRIRAMGWQAATSLEESIRRGYEWYLAN
HHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]