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The map label for this gene is paaF [C]

Identifier: 83309774

GI number: 83309774

Start: 725427

End: 726209

Strand: Reverse

Name: paaF [C]

Synonym: amb0675

Alternate gene names: 83309774

Gene position: 726209-725427 (Counterclockwise)

Preceding gene: 83309775

Following gene: 83309773

Centisome position: 14.62

GC content: 70.63

Gene sequence:

>783_bases
ATGAGCGCCCTTCTCGTCACCACCCATGACGGCGTGCGGACCATCACCCTCAATCGGGCCGAGCGGCACAACGCCTTCGA
TGACGCCCTGATCGCCGAACTGGCCGCCGCCTTCCGCGACGCCGCCACGGCGCCGGGCATCCGCGCCGTGGTGCTGGACT
CCACGGGAAAAAGCTTTTCCGCCGGGGCCGACCTGGAGTGGATGAAGCGCATGGCGGGCTATTCCCACGAGGAAAACCTG
GCAGACGCCCAAGCCCTGTCGGACATGCTGGAAGCCATCGACGCCTGCCCCCGCCCCGTCATCGGCGTGGTGCAGGGTGC
CGCCTATGGCGGCGGCGTCGGGCTGGTGGCCTGCTGCGATCTGGCCGTCGCGGCGGAAGGCGCGACCTTCTGCCTGTCCG
AGGTCAAGCTGGGCATCATCCCGGCGGTGATCAGCCCCTATGTGGTGCGCGCCATGGGCGGCCGCGCCGCCCGGCGCTAC
GCCGTCACCGCCGAACTGTTCGACGCGGCCGAGGCCAAGGCCACCGGGCTGGTGCACGAGGTCGTCCCCGCCGAGCATCT
GGCCGAGGTCAGGGACAAGTGGCTGGCCCGCGTCAAGGGCAACGGGCCGCAGGCCATGAGCGCCGCCAAGGGCCTGATCC
GCCGCGCCGCCGACCAGCCCCTGGACGAGTCCTTGCGGGCCTGGACCGCCGGCCAGATCGCCGAGCGCCGGGCCAGCGAC
GAGGGCAAGGAAGGCATCCGCGCCTTCCTGGAAAAGCGCAAACCCGCTTGGATTGAGGGATAA

Upstream 100 bases:

>100_bases
ATCCGGCCGAGACCCGCATGGTGCTGGCGCTGTCGCTGTCCGCCTCCTTCAACGCCCCCATCGAGCCGACCAAGTTCGGC
GTGTTCAGGATGTAAGCGGC

Downstream 100 bases:

>100_bases
AGAGGCATGTTCGATAAGATCCTCATCGCCAATCGCGGCGAAATCGCCTGCCGGGTCATGCGCACCGCCAAGCGCCTCGG
GATTCGCACCGTGGCGGTCT

Product: enoyl-CoA hydratase/carnithine racemase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSALLVTTHDGVRTITLNRAERHNAFDDALIAELAAAFRDAATAPGIRAVVLDSTGKSFSAGADLEWMKRMAGYSHEENL
ADAQALSDMLEAIDACPRPVIGVVQGAAYGGGVGLVACCDLAVAAEGATFCLSEVKLGIIPAVISPYVVRAMGGRAARRY
AVTAELFDAAEAKATGLVHEVVPAEHLAEVRDKWLARVKGNGPQAMSAAKGLIRRAADQPLDESLRAWTAGQIAERRASD
EGKEGIRAFLEKRKPAWIEG

Sequences:

>Translated_260_residues
MSALLVTTHDGVRTITLNRAERHNAFDDALIAELAAAFRDAATAPGIRAVVLDSTGKSFSAGADLEWMKRMAGYSHEENL
ADAQALSDMLEAIDACPRPVIGVVQGAAYGGGVGLVACCDLAVAAEGATFCLSEVKLGIIPAVISPYVVRAMGGRAARRY
AVTAELFDAAEAKATGLVHEVVPAEHLAEVRDKWLARVKGNGPQAMSAAKGLIRRAADQPLDESLRAWTAGQIAERRASD
EGKEGIRAFLEKRKPAWIEG
>Mature_259_residues
SALLVTTHDGVRTITLNRAERHNAFDDALIAELAAAFRDAATAPGIRAVVLDSTGKSFSAGADLEWMKRMAGYSHEENLA
DAQALSDMLEAIDACPRPVIGVVQGAAYGGGVGLVACCDLAVAAEGATFCLSEVKLGIIPAVISPYVVRAMGGRAARRYA
VTAELFDAAEAKATGLVHEVVPAEHLAEVRDKWLARVKGNGPQAMSAAKGLIRRAADQPLDESLRAWTAGQIAERRASDE
GKEGIRAFLEKRKPAWIEG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=32.1285140562249, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=28.6290322580645, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=28.4584980237154, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI45643119, Length=258, Percent_Identity=28.2945736434109, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260275230, Length=258, Percent_Identity=28.2945736434109, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260274832, Length=258, Percent_Identity=28.2945736434109, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI157694516, Length=222, Percent_Identity=23.8738738738739, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI213417737, Length=222, Percent_Identity=23.8738738738739, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=30.9803921568627, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=268, Percent_Identity=32.4626865671642, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=30.6451612903226, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=28.6259541984733, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=28.7822878228782, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=31.1538461538462, Blast_Score=88, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=32.183908045977, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=30.4, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=211, Percent_Identity=30.8056872037915, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=234, Percent_Identity=29.4871794871795, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=239, Percent_Identity=24.6861924686192, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=28.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=27.5590551181102, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=238, Percent_Identity=24.7899159663866, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=31.7269076305221, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=31.7269076305221, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=32.6923076923077, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=28.957528957529, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920382, Length=186, Percent_Identity=31.7204301075269, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27477; Mature: 27345

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLVTTHDGVRTITLNRAERHNAFDDALIAELAAAFRDAATAPGIRAVVLDSTGKSFS
CCEEEEEECCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
AGADLEWMKRMAGYSHEENLADAQALSDMLEAIDACPRPVIGVVQGAAYGGGVGLVACCD
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHH
LAVAAEGATFCLSEVKLGIIPAVISPYVVRAMGGRAARRYAVTAELFDAAEAKATGLVHE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPAEHLAEVRDKWLARVKGNGPQAMSAAKGLIRRAADQPLDESLRAWTAGQIAERRASD
HCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
EGKEGIRAFLEKRKPAWIEG
CHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SALLVTTHDGVRTITLNRAERHNAFDDALIAELAAAFRDAATAPGIRAVVLDSTGKSFS
CEEEEEECCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
AGADLEWMKRMAGYSHEENLADAQALSDMLEAIDACPRPVIGVVQGAAYGGGVGLVACCD
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHH
LAVAAEGATFCLSEVKLGIIPAVISPYVVRAMGGRAARRYAVTAELFDAAEAKATGLVHE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPAEHLAEVRDKWLARVKGNGPQAMSAAKGLIRRAADQPLDESLRAWTAGQIAERRASD
HCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
EGKEGIRAFLEKRKPAWIEG
CHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA