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The map label for this gene is ubiE [H]

Identifier: 83309730

GI number: 83309730

Start: 680116

End: 680739

Strand: Reverse

Name: ubiE [H]

Synonym: amb0631

Alternate gene names: 83309730

Gene position: 680739-680116 (Counterclockwise)

Preceding gene: 83309741

Following gene: 83309729

Centisome position: 13.7

GC content: 68.11

Gene sequence:

>624_bases
ATGAGCGCCGGCGTGCACCGCCTGTGGAAGTCGGCGTTCCTCGACATGCTGCGGCCCCGGCCCGACCAGACCCTGCTGGA
CGTGGGCGGCGGCACCGGCGACATCGCCTTCGGCTGGCGAAAGCGCGGTGGCGGCCCCGTCACCGTCTGCGACATCAACC
GCGAGATGCTGTCCGTCGGCCGCGACCGCGCCGTGGACCGCAATCTGGTGGGCGGCCTGACCTGGGTGTGCGGCAATGCC
GAGGACTTGCCCATACCCGACCGGTCGGTCGACCGCTACACCATCGCCTTCTGCCTGCGCAACGTCACCCATTGGGACAA
GGCCATCGCCGAGGCCTATCGGGTGCTGCGTCCCGGCGGGCGCTTCATGTGCCTGGAATTCTCCCGCGTCATCGTGCCGG
GCCTGCGCGAGGCCTATGACGCCTATTCCTTCAACGTGCTGCCCAAGGTGGGCGGCATGGTCACCGGCAATGCCGAGGCC
TATCAGTATCTGGTGGAAAGCATCCGCAAGTTCCCGCCCCAGGAGGAAATGGCAGCCATGGTCGAGGCCGCCGGCTTCTC
CCGCGTCGAGGTCCGCAACCTGTCGGCGGGCATCGCCGCCATCCATTCGGGGTGGCGGATATGA

Upstream 100 bases:

>100_bases
CCATTTCGGCTTCAAGACCGTGGCCGAGGACGAGAAGGTTTCCCTCGTCCGCGGCGTCTTCGATTCGGTGGCGTCCAAAT
ACGACCTGATGAACGATCTG

Downstream 100 bases:

>100_bases
TGGCGCCCGGTGAGGCGAACCGTCATCCCGACGACCATCGGGAGGAGGGATCTCGTCCTGATCCGGCGGTTCCGAATCGG
ACCCGGCAGTCCAAGCGGAG

Product: methylase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSAGVHRLWKSAFLDMLRPRPDQTLLDVGGGTGDIAFGWRKRGGGPVTVCDINREMLSVGRDRAVDRNLVGGLTWVCGNA
EDLPIPDRSVDRYTIAFCLRNVTHWDKAIAEAYRVLRPGGRFMCLEFSRVIVPGLREAYDAYSFNVLPKVGGMVTGNAEA
YQYLVESIRKFPPQEEMAAMVEAAGFSRVEVRNLSAGIAAIHSGWRI

Sequences:

>Translated_207_residues
MSAGVHRLWKSAFLDMLRPRPDQTLLDVGGGTGDIAFGWRKRGGGPVTVCDINREMLSVGRDRAVDRNLVGGLTWVCGNA
EDLPIPDRSVDRYTIAFCLRNVTHWDKAIAEAYRVLRPGGRFMCLEFSRVIVPGLREAYDAYSFNVLPKVGGMVTGNAEA
YQYLVESIRKFPPQEEMAAMVEAAGFSRVEVRNLSAGIAAIHSGWRI
>Mature_206_residues
SAGVHRLWKSAFLDMLRPRPDQTLLDVGGGTGDIAFGWRKRGGGPVTVCDINREMLSVGRDRAVDRNLVGGLTWVCGNAE
DLPIPDRSVDRYTIAFCLRNVTHWDKAIAEAYRVLRPGGRFMCLEFSRVIVPGLREAYDAYSFNVLPKVGGMVTGNAEAY
QYLVESIRKFPPQEEMAAMVEAAGFSRVEVRNLSAGIAAIHSGWRI

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=242, Percent_Identity=47.5206611570248, Blast_Score=225, Evalue=2e-59,
Organism=Escherichia coli, GI48994983, Length=209, Percent_Identity=47.3684210526316, Blast_Score=206, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17557039, Length=209, Percent_Identity=46.88995215311, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323526, Length=224, Percent_Identity=41.5178571428571, Blast_Score=157, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24641702, Length=222, Percent_Identity=48.6486486486487, Blast_Score=219, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 22905; Mature: 22773

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGVHRLWKSAFLDMLRPRPDQTLLDVGGGTGDIAFGWRKRGGGPVTVCDINREMLSVG
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCCCCCCCEEEEEECCHHHHHCC
RDRAVDRNLVGGLTWVCGNAEDLPIPDRSVDRYTIAFCLRNVTHWDKAIAEAYRVLRPGG
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RFMCLEFSRVIVPGLREAYDAYSFNVLPKVGGMVTGNAEAYQYLVESIRKFPPQEEMAAM
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHH
VEAAGFSRVEVRNLSAGIAAIHSGWRI
HHHCCCCCEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAGVHRLWKSAFLDMLRPRPDQTLLDVGGGTGDIAFGWRKRGGGPVTVCDINREMLSVG
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCCCCCCCEEEEEECCHHHHHCC
RDRAVDRNLVGGLTWVCGNAEDLPIPDRSVDRYTIAFCLRNVTHWDKAIAEAYRVLRPGG
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RFMCLEFSRVIVPGLREAYDAYSFNVLPKVGGMVTGNAEAYQYLVESIRKFPPQEEMAAM
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHH
VEAAGFSRVEVRNLSAGIAAIHSGWRI
HHHCCCCCEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA