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The map label for this gene is hldD [H]

Identifier: 83309695

GI number: 83309695

Start: 642853

End: 643863

Strand: Reverse

Name: hldD [H]

Synonym: amb0596

Alternate gene names: 83309695

Gene position: 643863-642853 (Counterclockwise)

Preceding gene: 83309696

Following gene: 83309694

Centisome position: 12.96

GC content: 63.9

Gene sequence:

>1011_bases
ATGATCCCACTGGCGGAGAGCCTCATGATCCTCGTCACCGGCGGAGCCGGCTTCATCGGTTCCAACATCCTGGCGGCCCT
CGAGGAAAAGGGGGCGGGCAAGCTGGTGGTTTGCGACCGCCTGCGCTCGGGCACCAAGTGGCAAAACATCGCCAAGCGCG
AACTGGCCGACATCGTCCATCCCGAGCAGATCTTCGATTTCCTCGAAGCCAATGCCAAGCACATGGAGGTGGTGATCCAC
ATGGGGGCCATCTCGGCCACCACCGAGACCGACGCCGACAAGATCCTCGCCAACAACTTCTCGCTGTCCCTGGCCCTTTG
GAAGTGGTGCGCGCTGCACGACGTGCGCTTTATCTACGCCTCGTCGGCCGCCACCTATGGCGACGGCACCCAAGGCTTCG
ACGATGACGGCTCCATGGAGCATCTGGCCCATCTGCGTCCCTTGAACGCCTATGGCTGGTCCAAGCACCTGTTCGACCGC
CGGGTGGCGCGCAAGGTGTGGGCCGGCTCGCGCAAGCCGCCGCAATGGGCGGGCCTCAAGTTCTTCAACGTCTACGGCCC
CAACGAGTATCACAAGGGTGGCCAGCAGAGCGTGGTGGCCCAGGTCTATCCCCATGCCGAGAAGAACGCCGCCTACCAGC
TGTTCCGCTCGCACAATCCCAAATACCCCGATGGCGGCCAGCTGCGTGATTTCATCTGGATCGGCGACGTGGTCGACGTG
GTGATGTGGCTGGTGGAGAACCCCAAGGTCAGCGGGCTGTTCAACGTGGGCACCGGCAAGGCGCGCTCGTTCCTCGACCT
GGCCAACGCCGTCTACCGCGCCGTGGGCCGCGAGCCGCAGATCAAGTTCCGCGACACCCCCATCGAGATCCGCGACAAGT
ACCAGTACTTCACCCAGGCCAAGATGGAGCGTCTGCACCGCGCCGGCTATTCCAAGCCCTTCACCTCGCTGGAAGACGGC
GTCGAGCAGTACGTGAAGCGCTACCTCGCCTCCGGGGATGCCTACCGGTGA

Upstream 100 bases:

>100_bases
CGGCATCCGCGCCGGGCTGGTCGACGCGGTCGAGCGCATGAAGATGCGGATCAGACGGTAACTTGCGAGCCCGCACGTCC
CGGCCTATAAGAGTCGCGGA

Downstream 100 bases:

>100_bases
TGACCTTCGCCCTGGCCTATCCCCATATCGACCCCATCGCGCTGCAGATCGGACCCATCGCCATCCGCTGGTACGCCCTG
GCCTATATCGCCGGGCTGAT

Product: nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MIPLAESLMILVTGGAGFIGSNILAALEEKGAGKLVVCDRLRSGTKWQNIAKRELADIVHPEQIFDFLEANAKHMEVVIH
MGAISATTETDADKILANNFSLSLALWKWCALHDVRFIYASSAATYGDGTQGFDDDGSMEHLAHLRPLNAYGWSKHLFDR
RVARKVWAGSRKPPQWAGLKFFNVYGPNEYHKGGQQSVVAQVYPHAEKNAAYQLFRSHNPKYPDGGQLRDFIWIGDVVDV
VMWLVENPKVSGLFNVGTGKARSFLDLANAVYRAVGREPQIKFRDTPIEIRDKYQYFTQAKMERLHRAGYSKPFTSLEDG
VEQYVKRYLASGDAYR

Sequences:

>Translated_336_residues
MIPLAESLMILVTGGAGFIGSNILAALEEKGAGKLVVCDRLRSGTKWQNIAKRELADIVHPEQIFDFLEANAKHMEVVIH
MGAISATTETDADKILANNFSLSLALWKWCALHDVRFIYASSAATYGDGTQGFDDDGSMEHLAHLRPLNAYGWSKHLFDR
RVARKVWAGSRKPPQWAGLKFFNVYGPNEYHKGGQQSVVAQVYPHAEKNAAYQLFRSHNPKYPDGGQLRDFIWIGDVVDV
VMWLVENPKVSGLFNVGTGKARSFLDLANAVYRAVGREPQIKFRDTPIEIRDKYQYFTQAKMERLHRAGYSKPFTSLEDG
VEQYVKRYLASGDAYR
>Mature_336_residues
MIPLAESLMILVTGGAGFIGSNILAALEEKGAGKLVVCDRLRSGTKWQNIAKRELADIVHPEQIFDFLEANAKHMEVVIH
MGAISATTETDADKILANNFSLSLALWKWCALHDVRFIYASSAATYGDGTQGFDDDGSMEHLAHLRPLNAYGWSKHLFDR
RVARKVWAGSRKPPQWAGLKFFNVYGPNEYHKGGQQSVVAQVYPHAEKNAAYQLFRSHNPKYPDGGQLRDFIWIGDVVDV
VMWLVENPKVSGLFNVGTGKARSFLDLANAVYRAVGREPQIKFRDTPIEIRDKYQYFTQAKMERLHRAGYSKPFTSLEDG
VEQYVKRYLASGDAYR

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=321, Percent_Identity=40.809968847352, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786974, Length=302, Percent_Identity=25.8278145695364, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115532424, Length=340, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=298, Percent_Identity=25.8389261744966, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 37790; Mature: 37790

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLAESLMILVTGGAGFIGSNILAALEEKGAGKLVVCDRLRSGTKWQNIAKRELADIVH
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHHHHHHHHCC
PEQIFDFLEANAKHMEVVIHMGAISATTETDADKILANNFSLSLALWKWCALHDVRFIYA
HHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHEEE
SSAATYGDGTQGFDDDGSMEHLAHLRPLNAYGWSKHLFDRRVARKVWAGSRKPPQWAGLK
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
FFNVYGPNEYHKGGQQSVVAQVYPHAEKNAAYQLFRSHNPKYPDGGQLRDFIWIGDVVDV
EEEECCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEHHEEHHHHHHH
VMWLVENPKVSGLFNVGTGKARSFLDLANAVYRAVGREPQIKFRDTPIEIRDKYQYFTQA
HHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
KMERLHRAGYSKPFTSLEDGVEQYVKRYLASGDAYR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIPLAESLMILVTGGAGFIGSNILAALEEKGAGKLVVCDRLRSGTKWQNIAKRELADIVH
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHHHHHHHHCC
PEQIFDFLEANAKHMEVVIHMGAISATTETDADKILANNFSLSLALWKWCALHDVRFIYA
HHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHEEE
SSAATYGDGTQGFDDDGSMEHLAHLRPLNAYGWSKHLFDRRVARKVWAGSRKPPQWAGLK
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
FFNVYGPNEYHKGGQQSVVAQVYPHAEKNAAYQLFRSHNPKYPDGGQLRDFIWIGDVVDV
EEEECCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEHHEEHHHHHHH
VMWLVENPKVSGLFNVGTGKARSFLDLANAVYRAVGREPQIKFRDTPIEIRDKYQYFTQA
HHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
KMERLHRAGYSKPFTSLEDGVEQYVKRYLASGDAYR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]