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The map label for this gene is nasD [H]

Identifier: 83309632

GI number: 83309632

Start: 576100

End: 578559

Strand: Reverse

Name: nasD [H]

Synonym: amb0533

Alternate gene names: 83309632

Gene position: 578559-576100 (Counterclockwise)

Preceding gene: 83309633

Following gene: 83309631

Centisome position: 11.65

GC content: 67.89

Gene sequence:

>2460_bases
ATGAACGCTCTTCCCCGTCAGAAGCTGGTCGTCATCGGCAACGGCATGGCCGGCATGCGCACCGTCGAGGAGCTGCTGGC
CATCGCGCCGGCCCGTTACGACATCACGGTGTTCGGCGCCGAGCCCCACCCCAATTACAACCGCATCATGCTGTCCTCGG
TGCTGGCCGGCGAAAAGCAGGTGGACGACATCGTCATCAACCCGCGCGCCTGGTACGCCGAGAACGGAATCACCCTGCAC
ACCGGCGACCCGGTGGTGGCCATCGATCGCGCCGCCAAGACCGTCACCTCGGCCAATGGCGTGGTGGTGCCCTATGACAA
GCTGCTGCTGTCCACCGGCTCCAAGCCGCTGATGCCGCCGCTGCCCGGCCTTGACCTGCCCGGCGTGGTGGCCTTCCGCG
ACATCGCCGACGTGGAGAAGATGCTGGACGCCGCCGAGGCCAAGCAGCGCGCCGTGGTGATCGGCGGCGGCCTGCTGGGT
CTGGAGGCCGCCTGGGGCCTGAAGCGGCGCGGCATGCCCGTCGCCCTGGTCCACCTGATGCCCACCCTGATGGAGCGCCA
GCTGGATGTGGAGGCCGGCGGCCTGCTGCAGAAGGACCTGACCGAGCGCGGCCTGCACTTCTTCACCTCGGGCCAGACCG
AGGAAATCATGGGCGCCGAAACCGGGCGCGCCCAGGGCGTCAAGCTCACCGACGGCCGCGAGATTCCCGCCGATCTGGTG
GTGGTGGCCATCGGCATCCGCCCCAACGTGGATCTGGCCAAGGCCGCGGGCCTCGACATCAACCGCGGCATCGAGGTGGG
CGACGACATGGCCACCTCGGACCCCGCCATCTATTCGGTGGGCGAGTGCGTCGAGCATCGCGGCCAGATCTTCGGGCTGG
TGGCGCCCATCTGGGAGCAGGCCAAGGTCTGCGCCTCGCGTCTGGCGGGCCGCGACGACGCCCATTACGAGACCCCGCCA
CTGTCCACCCGGCTCAAGATCACCGGCATCGACGTGTTCTCGGCTGGACAGCTGGCGGCCCAGGACGAAGCCGACGAGGA
GCTGGTGTATCGGGACTCCGCCCGGGGCATCTACAAGAAGCTGGTGATCCGCGCCGACAAGGTGGTGGGCGCCGTGATGT
ACGGCGACGTGGCCGACGGCTCCTGGTACTTCCAGCTCATCCGCGAAAAGGCCGACGTGGCCGCCATCCGCGACCGCATG
ATCTTCGGCCAGGCCTATGCCGATACCACCTGCAAGGGCCACGCGGGCGGCGTCAATGTGGCCGCCACGAGTGACGATGC
CCAGGTCTGCGGCTGTAACGGCGTGTCCAAGGGCGCCATCGTCAAGGCCATCACCGAGAAGGGTCTGACCTCGCTCGACG
AGGTCAAGCTGCACACCAAGGCCTCGGCCTCCTGCGGCCAGTGCGCCTCGGTGGTCCAGGCCATCCTGACCCACGTCACC
GGCGAGGTGGTGGACGCCAAGGCCGCCGGCATGTGCGGCTGCACCGACCTCTCCCACGACGAGGTGCGCAGGGAGATCCT
GCACCAGGGGCTCAAGACCCAGGACGCGGTACGGCAGGCTTTGGGCTGGCGCCATGCCGACGGCTGCGCCAAGTGCCGCC
CGGCCCTGAACTACTACCTGCTGTGCGCCTGGCCCAGTGAATACGTCGACGACTACCAGAGCCGCTTCATCAACGAGCGT
GCCCACGCCAATATCCAGAAGGACGGCACCTATTCGGTCATTCCGCGCATGTGGGGCGGCCTGACCAATCCCACCGAACT
GCGCGCCATCGCCGACGTGGTGGACAAGTTCAAGATCCCCACCGTCAAGGTCACCGGCGGCCAGCGCCTCGACCTGCTGG
GGGTCAGGAAGGAGGATCTGCCCGGCGTGTGGGCCGTGCTGGGCAAGGCCGGTCTGGTCTCGGGCCACGGCTACGCCAAG
GGCCTGCGCACGGTGAAGACCTGCGTCGGCTCGGAATGGTGCCGCTTCGGCACCCAGGATTCCACCGGCCTCGGCGTCAA
GCTGGAGAAGCTGATGTGGGGCTCGTGGACACCGCACAAGGTCAAGCTGGCGGTATCGGGCTGCCCGCGCAACTGCGCCG
AGGCGACCATCAAGGATATGGGCGTGGTCTGCGTCGAGGCGGGCTACGACATCTCGGTGGGCGGCAACGGCGGCATCGAG
CTGCGCGGCACCGACCATCTGGTCCGCGTCACCTCCGAGGAGGAGGTGCTCGAATATTGCGGCGCCTTCATGCAGATCTA
CCGCGAGGAAGCCCGCTACCTGGAGCGCACCGCGCCGTGGGTCGAGCGGGTCGGCATGGACTACATCACCAAGCGCGTCG
TCGAGGATGCCGAAGGCCGCCGCGCCGCCTTCGCCCGCTTCGTCTTCTCGCAGAACTACGCGCAGATCGATCCCTGGGCC
GAACGGGCCTCGGGCGAGGTGGATGCGCACGAATTCAAAACCCTGGCCGAGGTGGGCTAA

Upstream 100 bases:

>100_bases
TGGGGCCCGACCTGCTGCTGGGCGGCGGGACCTTCGACCCCTTCGCATCCCGCGCCACCGCCATGCCCCCGACGGCAAAG
ACCGAATCAGGAGACCTCAC

Downstream 100 bases:

>100_bases
GACCATGCAGGACTGGATCAAGATCGGACGCGTCGAGGACATCCCCAGGCTGGGGGCCCGCACCGTCCAGACGGACAAGG
GCGACATCGCGGTCTTCCGC

Product: NAD(P)H-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 819; Mature: 819

Protein sequence:

>819_residues
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQVDDIVINPRAWYAENGITLH
TGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPPLPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLG
LEAAWGLKRRGMPVALVHLMPTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQAKVCASRLAGRDDAHYETPP
LSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKKLVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRM
IFGQAYADTTCKGHAGGVNVAATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYLLCAWPSEYVDDYQSRFINER
AHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIPTVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAK
GLRTVKTCVGSEWCRFGTQDSTGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGRRAAFARFVFSQNYAQIDPWA
ERASGEVDAHEFKTLAEVG

Sequences:

>Translated_819_residues
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQVDDIVINPRAWYAENGITLH
TGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPPLPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLG
LEAAWGLKRRGMPVALVHLMPTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQAKVCASRLAGRDDAHYETPP
LSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKKLVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRM
IFGQAYADTTCKGHAGGVNVAATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYLLCAWPSEYVDDYQSRFINER
AHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIPTVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAK
GLRTVKTCVGSEWCRFGTQDSTGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGRRAAFARFVFSQNYAQIDPWA
ERASGEVDAHEFKTLAEVG
>Mature_819_residues
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQVDDIVINPRAWYAENGITLH
TGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPPLPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLG
LEAAWGLKRRGMPVALVHLMPTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQAKVCASRLAGRDDAHYETPP
LSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKKLVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRM
IFGQAYADTTCKGHAGGVNVAATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYLLCAWPSEYVDDYQSRFINER
AHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIPTVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAK
GLRTVKTCVGSEWCRFGTQDSTGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGRRAAFARFVFSQNYAQIDPWA
ERASGEVDAHEFKTLAEVG

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=278, Percent_Identity=28.4172661870504, Blast_Score=101, Evalue=4e-21,
Organism=Homo sapiens, GI226437568, Length=278, Percent_Identity=28.4172661870504, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI65787454, Length=278, Percent_Identity=28.4172661870504, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI22202629, Length=237, Percent_Identity=27.4261603375527, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4757732, Length=237, Percent_Identity=27.4261603375527, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=824, Percent_Identity=35.8009708737864, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1789065, Length=335, Percent_Identity=30.1492537313433, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1788892, Length=251, Percent_Identity=28.2868525896414, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=292, Percent_Identity=25.6849315068493, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564386, Length=295, Percent_Identity=27.1186440677966, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281359715, Length=276, Percent_Identity=27.1739130434783, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281359713, Length=276, Percent_Identity=27.1739130434783, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639250, Length=276, Percent_Identity=27.1739130434783, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18543267, Length=276, Percent_Identity=27.1739130434783, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639252, Length=276, Percent_Identity=27.1739130434783, Blast_Score=109, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639257, Length=276, Percent_Identity=27.1739130434783, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585130, Length=279, Percent_Identity=25.8064516129032, Blast_Score=106, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88506; Mature: 88506

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQ
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCC
VDDIVINPRAWYAENGITLHTGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPP
CCEEEECCCCEEECCCEEEECCCCEEEEEHHHHHHHCCCCEEECHHHHHHHCCCCCCCCC
LPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLGLEAAWGLKRRGMPVALVHLM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCHHHCCCCCHHHHHH
PTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
HHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCHHHHCCCCCCCCCEEECCCCCCCCCEE
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQ
EEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHH
AKVCASRLAGRDDAHYETPPLSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKK
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHCHHHHHHHH
LVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRMIFGQAYADTTCKGHAGGVNV
HHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEE
AATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
EECCCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHC
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYL
CHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCEEE
LCAWPSEYVDDYQSRFINERAHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIP
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHCCC
TVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAKGLRTVKTCVGSEWCRFGTQD
EEEECCCCEEEEECCCCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHHHCCCCC
STGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
CCCCCEEEHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEECCCEEEECCCCCEE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGR
EECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCH
RAAFARFVFSQNYAQIDPWAERASGEVDAHEFKTLAEVG
HHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQ
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCC
VDDIVINPRAWYAENGITLHTGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPP
CCEEEECCCCEEECCCEEEECCCCEEEEEHHHHHHHCCCCEEECHHHHHHHCCCCCCCCC
LPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLGLEAAWGLKRRGMPVALVHLM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCHHHCCCCCHHHHHH
PTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
HHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCHHHHCCCCCCCCCEEECCCCCCCCCEE
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQ
EEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHH
AKVCASRLAGRDDAHYETPPLSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKK
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHCHHHHHHHH
LVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRMIFGQAYADTTCKGHAGGVNV
HHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEE
AATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
EECCCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHC
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYL
CHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCEEE
LCAWPSEYVDDYQSRFINERAHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIP
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHCCC
TVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAKGLRTVKTCVGSEWCRFGTQD
EEEECCCCEEEEECCCCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHHHCCCCC
STGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
CCCCCEEEHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEECCCEEEECCCCCEE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGR
EECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCH
RAAFARFVFSQNYAQIDPWAERASGEVDAHEFKTLAEVG
HHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]