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The map label for this gene is nasA [H]

Identifier: 83309630

GI number: 83309630

Start: 573203

End: 575758

Strand: Reverse

Name: nasA [H]

Synonym: amb0531

Alternate gene names: 83309630

Gene position: 575758-573203 (Counterclockwise)

Preceding gene: 83309631

Following gene: 83309628

Centisome position: 11.59

GC content: 70.07

Gene sequence:

>2556_bases
ATGCCCGAGATCGTCCGCACCACCTGTCCCTATTGCGGCGTCGGCTGCGGCGTCATCGCCGCCCGCCTTGATGACTCCGC
GCAATGGGTAGTGCGGGGCGATCCCGAGCACCCCGCCAATTTTGGCCGCCTGTGCGTCAAGGGCTCCAGTCTGGCCGAGA
CTCTCGACCTGGACGGCCGCCTGCTCCACCCCATGATCGGGGAGCAAACGGTGGCGTGGGAGCCGGCGCTGGATCTGGTG
GCGGAAAAGTTCACCGCCGCCATCGCCGAGCACGGGCCGGATTCCGTGGCCTTCTACGTCTCCGGCCAATTGCTGACCGA
GGATTACTACGTCGCCAACAAGCTGATGAAGGGCTTCATCGGCTCGGGCAATATCGACACCAATTCCCGGCTGTGCATGT
CGTCCACCGTGGCCGGTCATGTGCGCGCCTTCGGCTCGGACACCGTGCCCGGCTGCTACGAGGACCTGGATCTGGCCGAT
CTGGTGGTGCTGGTAGGCTCCAATACCGCGTGGTGCCACCCTGTCGCCTTCCAGCGCATCCTGGCCGCCAAGGCCCGGCG
CCCCGACATGCGCATCGTGGTGGTCGATCCCCGCCGCACCGCCACCGCCGAATGCGCCGACCTGCATCTGGCCATCCGGC
CGGGCACCGACGCCATCCTGTTCAACGGGCTGCTGGCCCACCTCAGGGGCGAGGAAGCCCAGGTGGAGGTCGGCGTGCCG
CTGGAGCAGATCGTCCAGTTCTTCTCGTGGTTCGCCGAGACCGAAAAGACCGTCACGGTGTGGTCCCAGGGTATCAACCA
ATCCTCGTCGGGCACCGACAAGGTGGATGCCATCATCAACTGCCACCTGCAGACCGGCCGCATCGGCCGTCCCGGCATGG
GGCCGTTCTCACTGACCGGCCAGCCCAACGCCATGGGCGGGCGCGAGGTGGGGGGACTGGCCAACATGCTGGCCGCCCAT
ATGGGCTTCGACGAGGCCAATGTGGACCGGGTCGGGCGCTTCTGGGCGTCGGAGCGCGTGGCGGCCAAGCCCGGCCTCAA
GGCCGTCGACATGTTCAAGGCGGTGGCCGAGAGCAAGATCAAGGCCCTTTGGGTCATGTGCACCAACCCGGCGGTCAGCA
TGCCCGAGGCCGATCTGGTCCGGGCCGCCATCGCCGCCTGCCCCTTCGTGGTGGTCTCCGAATGCGAGGCCGACACCGAC
ACGGCCCGCCTCGCCCATGTGCGCCTGCCGGCGCTGACCTGGGGCGAAAAGGAGGGCACCGTCACCAATTCCGAGCGCCG
CATCTCGCGCCAGCGCCCCTTCCTCCCCACCCCGGGCGAGGCCAAGGGCGATTGGTGGATCATGGCCCAGGTGGCGCAGC
GCATGGGCTTTGGCGAGGCCTTCGCCTGGACCGATACGGGGGCCATCTTCGACGAGTTCTGCCGCCTGACCGCCTTCGAG
AACCATGGCGCCCGCGACCTGGATCTGTCGGGTCTGGTGGGAGCCGATTACGAGTCCATCGAGCCCATCCAGTGGCCGGT
GCGCGAACCGGGAAAGGGCACGGCGCGGCTGTTCGCCGACGGCCGCTTCTACCACCCCGATGGCAAGGCCCGGATGGTCG
ACACCCCGCCCCGCCCGCCAGCGCGGGCCACCGACGAGGCCTTTCCCCTGATCCTCAACACCGGGCGCATGCGCGACCAG
TGGCACACCATGACCCGCACCGGCCGTGCCGCTCGGCTGGCCGCCCACGCGCCCGAGCCGGTGCTGCAGGTCCATCCCCG
GGACGCCATCCGCTTCCGGCTGGCGGACGGGGGACTGGCCAAGGTCTCCGGCCGCCGGGCCAGCGTGGTTCTGCGCATCG
CCTTCGACAACGGCCAGCGCCCGGGCGAGGTCTTCGCCCCCATCCACTGGAACGACCGCACCAGTTCGGGCGCGGTGGTC
GGCACCCTAATCGACGCCGCCACCGATCCCGTCTCGGGCCAGCCGGAACTGAAGCAGGCCCCGGTGATGGTCGAGCCCCT
GGACGCCGCCTGGCACGGCGTGCTGCTCAGCCGCAATCCGGTAGAGCTGCCCCGCTCGTTCTACTGGGCCAAGGCGGCGG
GTCAGGCCCACTCCATCTGGCGGCTGGCGGGCGAAGCCGGCGAGGACTGGCCCGTCACCGCCAAGCAATGGCTGGGCGCC
GACGGCGAATGGATCGAGTTCCTCGACCCCAATCGCGGCCACTATCGCGGCGCCCGTCTGGTGGACGGCCGCCTGGATGC
GGTGCTGTTCGTCTTTCCCTGGGCCACCGATTTCTCGCCCGACTGGGTGGCCGCCGCCTTCGGCCGCGCCGCCATCGCCG
GCGACGAGCGCGCCACCCTGGTGGCCGGCGCGCCGCCCGGCGGCGACCGGGGCCGCGACAAGACGGTGTGCGCCTGCTTC
AACGTGGGCCTGGAAGCCATCCGCGCCGCCATCCGCGACCACCGCCTGTCCAGCGCGGCCGAGGTGGGCGCCATGCTCAA
GGCGGGGACCAATTGCGGCTCGTGCGTGCCGGAAATCCGGGCGATCCTGGCCGAGGCGGCGCCCAAGGTGGCGTGA

Upstream 100 bases:

>100_bases
GCCCCCGACGAGGGCTGCACGCGGCCCGTGGATTCCAAGGTGGTCGACGGCCTGGTCTGGCTGAAGCTCGAAAGCGCGAA
AAAGGTGGCCCATGGCTGAC

Downstream 100 bases:

>100_bases
CTTATGGCGTCATGGCCGTGCTTGATACGGCCATGACGCCCTATACCTAGAAAACGGCCCAGCCCTTGTCGCCCTTGACG
AAGCGGGCCGAGGCCTTCTC

Product: anaerobic dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 851; Mature: 850

Protein sequence:

>851_residues
MPEIVRTTCPYCGVGCGVIAARLDDSAQWVVRGDPEHPANFGRLCVKGSSLAETLDLDGRLLHPMIGEQTVAWEPALDLV
AEKFTAAIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTNSRLCMSSTVAGHVRAFGSDTVPGCYEDLDLAD
LVVLVGSNTAWCHPVAFQRILAAKARRPDMRIVVVDPRRTATAECADLHLAIRPGTDAILFNGLLAHLRGEEAQVEVGVP
LEQIVQFFSWFAETEKTVTVWSQGINQSSSGTDKVDAIINCHLQTGRIGRPGMGPFSLTGQPNAMGGREVGGLANMLAAH
MGFDEANVDRVGRFWASERVAAKPGLKAVDMFKAVAESKIKALWVMCTNPAVSMPEADLVRAAIAACPFVVVSECEADTD
TARLAHVRLPALTWGEKEGTVTNSERRISRQRPFLPTPGEAKGDWWIMAQVAQRMGFGEAFAWTDTGAIFDEFCRLTAFE
NHGARDLDLSGLVGADYESIEPIQWPVREPGKGTARLFADGRFYHPDGKARMVDTPPRPPARATDEAFPLILNTGRMRDQ
WHTMTRTGRAARLAAHAPEPVLQVHPRDAIRFRLADGGLAKVSGRRASVVLRIAFDNGQRPGEVFAPIHWNDRTSSGAVV
GTLIDAATDPVSGQPELKQAPVMVEPLDAAWHGVLLSRNPVELPRSFYWAKAAGQAHSIWRLAGEAGEDWPVTAKQWLGA
DGEWIEFLDPNRGHYRGARLVDGRLDAVLFVFPWATDFSPDWVAAAFGRAAIAGDERATLVAGAPPGGDRGRDKTVCACF
NVGLEAIRAAIRDHRLSSAAEVGAMLKAGTNCGSCVPEIRAILAEAAPKVA

Sequences:

>Translated_851_residues
MPEIVRTTCPYCGVGCGVIAARLDDSAQWVVRGDPEHPANFGRLCVKGSSLAETLDLDGRLLHPMIGEQTVAWEPALDLV
AEKFTAAIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTNSRLCMSSTVAGHVRAFGSDTVPGCYEDLDLAD
LVVLVGSNTAWCHPVAFQRILAAKARRPDMRIVVVDPRRTATAECADLHLAIRPGTDAILFNGLLAHLRGEEAQVEVGVP
LEQIVQFFSWFAETEKTVTVWSQGINQSSSGTDKVDAIINCHLQTGRIGRPGMGPFSLTGQPNAMGGREVGGLANMLAAH
MGFDEANVDRVGRFWASERVAAKPGLKAVDMFKAVAESKIKALWVMCTNPAVSMPEADLVRAAIAACPFVVVSECEADTD
TARLAHVRLPALTWGEKEGTVTNSERRISRQRPFLPTPGEAKGDWWIMAQVAQRMGFGEAFAWTDTGAIFDEFCRLTAFE
NHGARDLDLSGLVGADYESIEPIQWPVREPGKGTARLFADGRFYHPDGKARMVDTPPRPPARATDEAFPLILNTGRMRDQ
WHTMTRTGRAARLAAHAPEPVLQVHPRDAIRFRLADGGLAKVSGRRASVVLRIAFDNGQRPGEVFAPIHWNDRTSSGAVV
GTLIDAATDPVSGQPELKQAPVMVEPLDAAWHGVLLSRNPVELPRSFYWAKAAGQAHSIWRLAGEAGEDWPVTAKQWLGA
DGEWIEFLDPNRGHYRGARLVDGRLDAVLFVFPWATDFSPDWVAAAFGRAAIAGDERATLVAGAPPGGDRGRDKTVCACF
NVGLEAIRAAIRDHRLSSAAEVGAMLKAGTNCGSCVPEIRAILAEAAPKVA
>Mature_850_residues
PEIVRTTCPYCGVGCGVIAARLDDSAQWVVRGDPEHPANFGRLCVKGSSLAETLDLDGRLLHPMIGEQTVAWEPALDLVA
EKFTAAIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTNSRLCMSSTVAGHVRAFGSDTVPGCYEDLDLADL
VVLVGSNTAWCHPVAFQRILAAKARRPDMRIVVVDPRRTATAECADLHLAIRPGTDAILFNGLLAHLRGEEAQVEVGVPL
EQIVQFFSWFAETEKTVTVWSQGINQSSSGTDKVDAIINCHLQTGRIGRPGMGPFSLTGQPNAMGGREVGGLANMLAAHM
GFDEANVDRVGRFWASERVAAKPGLKAVDMFKAVAESKIKALWVMCTNPAVSMPEADLVRAAIAACPFVVVSECEADTDT
ARLAHVRLPALTWGEKEGTVTNSERRISRQRPFLPTPGEAKGDWWIMAQVAQRMGFGEAFAWTDTGAIFDEFCRLTAFEN
HGARDLDLSGLVGADYESIEPIQWPVREPGKGTARLFADGRFYHPDGKARMVDTPPRPPARATDEAFPLILNTGRMRDQW
HTMTRTGRAARLAAHAPEPVLQVHPRDAIRFRLADGGLAKVSGRRASVVLRIAFDNGQRPGEVFAPIHWNDRTSSGAVVG
TLIDAATDPVSGQPELKQAPVMVEPLDAAWHGVLLSRNPVELPRSFYWAKAAGQAHSIWRLAGEAGEDWPVTAKQWLGAD
GEWIEFLDPNRGHYRGARLVDGRLDAVLFVFPWATDFSPDWVAAAFGRAAIAGDERATLVAGAPPGGDRGRDKTVCACFN
VGLEAIRAAIRDHRLSSAAEVGAMLKAGTNCGSCVPEIRAILAEAAPKVA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=795, Percent_Identity=30.5660377358491, Blast_Score=306, Evalue=4e-84,
Organism=Escherichia coli, GI3868721, Length=711, Percent_Identity=27.7074542897328, Blast_Score=262, Evalue=8e-71,
Organism=Escherichia coli, GI3868720, Length=252, Percent_Identity=32.1428571428571, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI3868719, Length=248, Percent_Identity=31.0483870967742, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1787778, Length=698, Percent_Identity=22.0630372492837, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787870, Length=238, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 91933; Mature: 91802

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIVRTTCPYCGVGCGVIAARLDDSAQWVVRGDPEHPANFGRLCVKGSSLAETLDLDGR
CCCCHHHCCCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHCCCCE
LLHPMIGEQTVAWEPALDLVAEKFTAAIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFI
EECHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEECHHHHHHHHHHHHC
GSGNIDTNSRLCMSSTVAGHVRAFGSDTVPGCYEDLDLADLVVLVGSNTAWCHPVAFQRI
CCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCEECHHHHHHH
LAAKARRPDMRIVVVDPRRTATAECADLHLAIRPGTDAILFNGLLAHLRGEEAQVEVGVP
HHHHCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCC
LEQIVQFFSWFAETEKTVTVWSQGINQSSSGTDKVDAIINCHLQTGRIGRPGMGPFSLTG
HHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEECC
QPNAMGGREVGGLANMLAAHMGFDEANVDRVGRFWASERVAAKPGLKAVDMFKAVAESKI
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCE
KALWVMCTNPAVSMPEADLVRAAIAACPFVVVSECEADTDTARLAHVRLPALTWGEKEGT
EEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHEEEEEECCCEECCCCCCC
VTNSERRISRQRPFLPTPGEAKGDWWIMAQVAQRMGFGEAFAWTDTGAIFDEFCRLTAFE
CCCHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHCCCCCEEEECCCHHHHHHHHHHHECC
NHGARDLDLSGLVGADYESIEPIQWPVREPGKGTARLFADGRFYHPDGKARMVDTPPRPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEECCCCCEEEECCCCCCC
ARATDEAFPLILNTGRMRDQWHTMTRTGRAARLAAHAPEPVLQVHPRDAIRFRLADGGLA
CCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCEEEEECCCCEE
KVSGRRASVVLRIAFDNGQRPGEVFAPIHWNDRTSSGAVVGTLIDAATDPVSGQPELKQA
EECCCEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEHHHCCCCCCCCCCHHCC
PVMVEPLDAAWHGVLLSRNPVELPRSFYWAKAAGQAHSIWRLAGEAGEDWPVTAKQWLGA
CEEEEECCHHHCCEEECCCCHHCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHCCC
DGEWIEFLDPNRGHYRGARLVDGRLDAVLFVFPWATDFSPDWVAAAFGRAAIAGDERATL
CCCEEEEECCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCEECCCCCEEE
VAGAPPGGDRGRDKTVCACFNVGLEAIRAAIRDHRLSSAAEVGAMLKAGTNCGSCVPEIR
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AILAEAAPKVA
HHHHHHCCCCC
>Mature Secondary Structure 
PEIVRTTCPYCGVGCGVIAARLDDSAQWVVRGDPEHPANFGRLCVKGSSLAETLDLDGR
CCCHHHCCCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHCCCCE
LLHPMIGEQTVAWEPALDLVAEKFTAAIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFI
EECHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEECHHHHHHHHHHHHC
GSGNIDTNSRLCMSSTVAGHVRAFGSDTVPGCYEDLDLADLVVLVGSNTAWCHPVAFQRI
CCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCEECHHHHHHH
LAAKARRPDMRIVVVDPRRTATAECADLHLAIRPGTDAILFNGLLAHLRGEEAQVEVGVP
HHHHCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCC
LEQIVQFFSWFAETEKTVTVWSQGINQSSSGTDKVDAIINCHLQTGRIGRPGMGPFSLTG
HHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEECC
QPNAMGGREVGGLANMLAAHMGFDEANVDRVGRFWASERVAAKPGLKAVDMFKAVAESKI
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCE
KALWVMCTNPAVSMPEADLVRAAIAACPFVVVSECEADTDTARLAHVRLPALTWGEKEGT
EEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHEEEEEECCCEECCCCCCC
VTNSERRISRQRPFLPTPGEAKGDWWIMAQVAQRMGFGEAFAWTDTGAIFDEFCRLTAFE
CCCHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHCCCCCEEEECCCHHHHHHHHHHHECC
NHGARDLDLSGLVGADYESIEPIQWPVREPGKGTARLFADGRFYHPDGKARMVDTPPRPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEECCCCCEEEECCCCCCC
ARATDEAFPLILNTGRMRDQWHTMTRTGRAARLAAHAPEPVLQVHPRDAIRFRLADGGLA
CCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCEEEEECCCCEE
KVSGRRASVVLRIAFDNGQRPGEVFAPIHWNDRTSSGAVVGTLIDAATDPVSGQPELKQA
EECCCEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEHHHCCCCCCCCCCHHCC
PVMVEPLDAAWHGVLLSRNPVELPRSFYWAKAAGQAHSIWRLAGEAGEDWPVTAKQWLGA
CEEEEECCHHHCCEEECCCCHHCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHCCC
DGEWIEFLDPNRGHYRGARLVDGRLDAVLFVFPWATDFSPDWVAAAFGRAAIAGDERATL
CCCEEEEECCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCEECCCCCEEE
VAGAPPGGDRGRDKTVCACFNVGLEAIRAAIRDHRLSSAAEVGAMLKAGTNCGSCVPEIR
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AILAEAAPKVA
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]