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The map label for this gene is rfaF [H]

Identifier: 83309627

GI number: 83309627

Start: 569948

End: 571162

Strand: Reverse

Name: rfaF [H]

Synonym: amb0528

Alternate gene names: 83309627

Gene position: 571162-569948 (Counterclockwise)

Preceding gene: 83309628

Following gene: 83309626

Centisome position: 11.5

GC content: 67.49

Gene sequence:

>1215_bases
GTGAACGTCGACAGGATGCGGCAGATCGATTTCTGGGCGGGGGTGCCCCTCGCGTTCCTGATCACCCTTTACTGGCGGAT
CCGCTGTTTCTTCAGCCCGCCGGCACCGTCCGCGGGCAAGAACATCCTGTTCATCGAACTGTCGGAGATGGGCAGCGCGG
TCATCGCCGACGCGGCCTTGAAGCGGGCGCAGGCGCTGTTCCCCGACGCCAGGGTCTATTTCCTGATCTTCGCCAAGAAC
CGTCCCAGCCTGGACATCATGGGCACCATCGCGCGCGAGAACATGCTGACCATCCGCGCCGACAGCCTGGTCACCCTGGT
CGTCGACACCCTGCGCATGATCCGCACCATGCGTTCGCTGGACCTGATGGCCACCATCGACATGGAGATGTTCTCGCGCT
TCTCGGCGCTGCTGACCTTCCTGTCGGGGGCGCCCAAGCGGGTGGGCTTTCACCGCTTCCACACCGAGGGCCTCTATCGG
GGCGAGTTGCTGACCCACCGGGTGGGCTACAACCCGCACCAGCACATCGCCAAGAGCTTCATGGCCCTGGTCCATGCCCT
GACCGAGCCCGAGGGCACCACGCCCCACGGCAAGGTCGCCTTCACCGACGACGAAGTGAGGCTGGCGCCGCTGGCCCCCG
CCCCCGAGGCGCTGGAGGCCTTCAAGACCCGGCTGTTTCAGGCCTATCCGGTGCTGAAGGAGACGACGCGCTGGGTGATC
CTCAATCCCAACAGCAGCGAGCTGATGCCGCTGCGCCGCTGGCCCTATGACCGCTACACCGAGGTGGCGCGGCGCCTGCT
GGCCGAGGACGAGGGCCTGGCCGTCATCGTCACCGGCGTGGCCTCGGAAAAGGCCGAGGCCCAGCGGCTGGTGGACGCCA
CCGGCTCGAACCGCGCCTGCAATCTGGCCGGCTTCACCCGCATGGAGGATCTGATTCCCCTCTATGCCCTGGCCCGAGCC
ATGGTCACCAACGATTCCGGCCCGGCCCATTTCGCCGCGCCGGTGGGCCTGCCCACCCTGGTGCTGTTCGGCCCCGAGAC
CCCCGCCCTCTACGGCGCCCTGAATCACAAGGCCGAGTTCATCACCGCCCGGCTGGCCTGCTCGCCCTGCGTCTCGGCCA
TGAATCACCGCAGCACCGCCTGCACCGACGCCGCGTGCATGCGGGCCATTTCGGTGGAGCAGGTCCTCGACGCGGTCAAG
CGCCTGCTGGGATGA

Upstream 100 bases:

>100_bases
ACGGTGGACCGGGCTTGGGGCGGCAAGGTCGTGGACCATTACGAAATCTGGCTGGCCGAGTCCTATGGCTCGGGCACCTT
CGAGAACAAGTGAGGCGGTC

Downstream 100 bases:

>100_bases
TGCTGATCGGCCATCGCGGCCTGGCCGGGCTGGCGCCCGAGAACACCCTGGCCTCGTTCAGGACCGCCGCCGCCCATGGC
CTCGCCATGGTGGAGTTCGA

Product: ADP-heptose

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MNVDRMRQIDFWAGVPLAFLITLYWRIRCFFSPPAPSAGKNILFIELSEMGSAVIADAALKRAQALFPDARVYFLIFAKN
RPSLDIMGTIARENMLTIRADSLVTLVVDTLRMIRTMRSLDLMATIDMEMFSRFSALLTFLSGAPKRVGFHRFHTEGLYR
GELLTHRVGYNPHQHIAKSFMALVHALTEPEGTTPHGKVAFTDDEVRLAPLAPAPEALEAFKTRLFQAYPVLKETTRWVI
LNPNSSELMPLRRWPYDRYTEVARRLLAEDEGLAVIVTGVASEKAEAQRLVDATGSNRACNLAGFTRMEDLIPLYALARA
MVTNDSGPAHFAAPVGLPTLVLFGPETPALYGALNHKAEFITARLACSPCVSAMNHRSTACTDAACMRAISVEQVLDAVK
RLLG

Sequences:

>Translated_404_residues
MNVDRMRQIDFWAGVPLAFLITLYWRIRCFFSPPAPSAGKNILFIELSEMGSAVIADAALKRAQALFPDARVYFLIFAKN
RPSLDIMGTIARENMLTIRADSLVTLVVDTLRMIRTMRSLDLMATIDMEMFSRFSALLTFLSGAPKRVGFHRFHTEGLYR
GELLTHRVGYNPHQHIAKSFMALVHALTEPEGTTPHGKVAFTDDEVRLAPLAPAPEALEAFKTRLFQAYPVLKETTRWVI
LNPNSSELMPLRRWPYDRYTEVARRLLAEDEGLAVIVTGVASEKAEAQRLVDATGSNRACNLAGFTRMEDLIPLYALARA
MVTNDSGPAHFAAPVGLPTLVLFGPETPALYGALNHKAEFITARLACSPCVSAMNHRSTACTDAACMRAISVEQVLDAVK
RLLG
>Mature_404_residues
MNVDRMRQIDFWAGVPLAFLITLYWRIRCFFSPPAPSAGKNILFIELSEMGSAVIADAALKRAQALFPDARVYFLIFAKN
RPSLDIMGTIARENMLTIRADSLVTLVVDTLRMIRTMRSLDLMATIDMEMFSRFSALLTFLSGAPKRVGFHRFHTEGLYR
GELLTHRVGYNPHQHIAKSFMALVHALTEPEGTTPHGKVAFTDDEVRLAPLAPAPEALEAFKTRLFQAYPVLKETTRWVI
LNPNSSELMPLRRWPYDRYTEVARRLLAEDEGLAVIVTGVASEKAEAQRLVDATGSNRACNLAGFTRMEDLIPLYALARA
MVTNDSGPAHFAAPVGLPTLVLFGPETPALYGALNHKAEFITARLACSPCVSAMNHRSTACTDAACMRAISVEQVLDAVK
RLLG

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=338, Percent_Identity=24.8520710059172, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 44783; Mature: 44783

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDRMRQIDFWAGVPLAFLITLYWRIRCFFSPPAPSAGKNILFIELSEMGSAVIADAAL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEHHHCCCHHHHHHHH
KRAQALFPDARVYFLIFAKNRPSLDIMGTIARENMLTIRADSLVTLVVDTLRMIRTMRSL
HHHHHHCCCCEEEEEEEECCCCCEEHEEEHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHH
DLMATIDMEMFSRFSALLTFLSGAPKRVGFHRFHTEGLYRGELLTHRVGYNPHQHIAKSF
HHHHEECHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHH
MALVHALTEPEGTTPHGKVAFTDDEVRLAPLAPAPEALEAFKTRLFQAYPVLKETTRWVI
HHHHHHHCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LNPNSSELMPLRRWPYDRYTEVARRLLAEDEGLAVIVTGVASEKAEAQRLVDATGSNRAC
ECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEE
NLAGFTRMEDLIPLYALARAMVTNDSGPAHFAAPVGLPTLVLFGPETPALYGALNHKAEF
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEECCCCCHHHHHHCCHHHH
ITARLACSPCVSAMNHRSTACTDAACMRAISVEQVLDAVKRLLG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNVDRMRQIDFWAGVPLAFLITLYWRIRCFFSPPAPSAGKNILFIELSEMGSAVIADAAL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEHHHCCCHHHHHHHH
KRAQALFPDARVYFLIFAKNRPSLDIMGTIARENMLTIRADSLVTLVVDTLRMIRTMRSL
HHHHHHCCCCEEEEEEEECCCCCEEHEEEHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHH
DLMATIDMEMFSRFSALLTFLSGAPKRVGFHRFHTEGLYRGELLTHRVGYNPHQHIAKSF
HHHHEECHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHH
MALVHALTEPEGTTPHGKVAFTDDEVRLAPLAPAPEALEAFKTRLFQAYPVLKETTRWVI
HHHHHHHCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LNPNSSELMPLRRWPYDRYTEVARRLLAEDEGLAVIVTGVASEKAEAQRLVDATGSNRAC
ECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEE
NLAGFTRMEDLIPLYALARAMVTNDSGPAHFAAPVGLPTLVLFGPETPALYGALNHKAEF
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEECCCCCHHHHHHCCHHHH
ITARLACSPCVSAMNHRSTACTDAACMRAISVEQVLDAVKRLLG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]