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The map label for this gene is mutT [H]

Identifier: 83309608

GI number: 83309608

Start: 545434

End: 546417

Strand: Reverse

Name: mutT [H]

Synonym: amb0509

Alternate gene names: 83309608

Gene position: 546417-545434 (Counterclockwise)

Preceding gene: 83309609

Following gene: 83309607

Centisome position: 11.0

GC content: 69.92

Gene sequence:

>984_bases
ATGGCGGAGCGGGGTTGCCGGGAACCGGGCACGGTCTGGGCGGGTCATCCCCTGCTCACGGCCCGTCTGCGCCTGCGGCC
GGTCAGCGACAAGGACCTGGACGCCCTGGTGGCGCTGCTGGGCGACTGGGATGTGGTGCGCCTCACCGCCGCCATTCCCC
ACCCCTATGCCGTGGACGACGCCAAGGCCTTCGTCGCCGCCCAGGAAGTCCGCCGCATCGAGCACAAGGGCGTCGCCCTG
GCCCTGGAGCGCACCACCGACGGCGTGCTGGTCGGCGTCATGGGCTTTGGCCTGGAACGGGACGGCACGCCGGAACTGGG
CTACTGGATCGGCAAGGATTTCTGGGGCCAGGGCTATGCCACCGAGGCGGCCAACCGCCTGATCCGCCATCTGTTCGACA
CCCTGGGCTTCGAACGGGTCTGGGCGCAGTTCCACCCCGACAACACCGGTTCCAAGCGGGTGCTGGAGAAGGTGGGACTC
ACCTTCCACCGGGTCGACGCCTGCCCCATGCCGGCGCGGGGCGAAAGCGTCATCTCTCCCGTCTTCCAGCTGCGCAGGGC
CGAGTGGCTGATGGCCCGCTCGCTCCGCCCCACCGTGCTGGTGGTGGCCGCCGCCCTGGTGGACGGCGACGGCCGCGTGC
TGATGGCGTCGCGCCCCACCGGCAAGAGCATGGAAGGCCTGTGGGAGTTCCCCGGCGGCAAGATTCACGACGGCGAGACC
CCGGAGGCCGCCCTGGTCCGCGAGTTGGAGGAGGAACTGGGCATCGACGTGCGGGAAAGCTGCCTAGCCCCCGTCGCCTT
CGCCTCCCACGATTACGACACCTTCCATCTGCTGATGCCGCTGTATCTGGTGCGGGTATGGAAGGGCAATCCCAGCGCCC
GCGAAGGCCAGGAACTGCGCTGGATCCGCGTGCCCCGCCTGGGCGACCTGCCCATGCCCCCCGCCGACATCCCCCTGGTC
GCCATCCTGCGGGAGTGGGTATAA

Upstream 100 bases:

>100_bases
GCGTGGGCAAGGGACGGTCGACCGTCTATACTTGCGACCTGACGCACGCGTATATCGACATCAATGGGTCCTATCGGACC
TGAGGAACGGAGACGGCATC

Downstream 100 bases:

>100_bases
TATCATTCCCTGATATGGGAGTGCGGTCATGGCCAATGCGGCGATCCTGGTGATCGGCAATGAAATCCTGTCGGGACGCA
CCCAGGATTCCAATGTCGCC

Product: NTP pyrophosphohydrolase

Products: CMP; diphosphate [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAERGCREPGTVWAGHPLLTARLRLRPVSDKDLDALVALLGDWDVVRLTAAIPHPYAVDDAKAFVAAQEVRRIEHKGVAL
ALERTTDGVLVGVMGFGLERDGTPELGYWIGKDFWGQGYATEAANRLIRHLFDTLGFERVWAQFHPDNTGSKRVLEKVGL
TFHRVDACPMPARGESVISPVFQLRRAEWLMARSLRPTVLVVAAALVDGDGRVLMASRPTGKSMEGLWEFPGGKIHDGET
PEAALVRELEEELGIDVRESCLAPVAFASHDYDTFHLLMPLYLVRVWKGNPSAREGQELRWIRVPRLGDLPMPPADIPLV
AILREWV

Sequences:

>Translated_327_residues
MAERGCREPGTVWAGHPLLTARLRLRPVSDKDLDALVALLGDWDVVRLTAAIPHPYAVDDAKAFVAAQEVRRIEHKGVAL
ALERTTDGVLVGVMGFGLERDGTPELGYWIGKDFWGQGYATEAANRLIRHLFDTLGFERVWAQFHPDNTGSKRVLEKVGL
TFHRVDACPMPARGESVISPVFQLRRAEWLMARSLRPTVLVVAAALVDGDGRVLMASRPTGKSMEGLWEFPGGKIHDGET
PEAALVRELEEELGIDVRESCLAPVAFASHDYDTFHLLMPLYLVRVWKGNPSAREGQELRWIRVPRLGDLPMPPADIPLV
AILREWV
>Mature_326_residues
AERGCREPGTVWAGHPLLTARLRLRPVSDKDLDALVALLGDWDVVRLTAAIPHPYAVDDAKAFVAAQEVRRIEHKGVALA
LERTTDGVLVGVMGFGLERDGTPELGYWIGKDFWGQGYATEAANRLIRHLFDTLGFERVWAQFHPDNTGSKRVLEKVGLT
FHRVDACPMPARGESVISPVFQLRRAEWLMARSLRPTVLVVAAALVDGDGRVLMASRPTGKSMEGLWEFPGGKIHDGETP
EAALVRELEEELGIDVRESCLAPVAFASHDYDTFHLLMPLYLVRVWKGNPSAREGQELRWIRVPRLGDLPMPPADIPLVA
ILREWV

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=125, Percent_Identity=37.6, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786288, Length=126, Percent_Identity=34.1269841269841, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 36358; Mature: 36227

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERGCREPGTVWAGHPLLTARLRLRPVSDKDLDALVALLGDWDVVRLTAAIPHPYAVDD
CCCCCCCCCCCEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCH
AKAFVAAQEVRRIEHKGVALALERTTDGVLVGVMGFGLERDGTPELGYWIGKDFWGQGYA
HHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHCHHHCCCCHH
TEAANRLIRHLFDTLGFERVWAQFHPDNTGSKRVLEKVGLTFHRVDACPMPARGESVISP
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
VFQLRRAEWLMARSLRPTVLVVAAALVDGDGRVLMASRPTGKSMEGLWEFPGGKIHDGET
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCEECCCCC
PEAALVRELEEELGIDVRESCLAPVAFASHDYDTFHLLMPLYLVRVWKGNPSAREGQELR
CHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
WIRVPRLGDLPMPPADIPLVAILREWV
EEECCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AERGCREPGTVWAGHPLLTARLRLRPVSDKDLDALVALLGDWDVVRLTAAIPHPYAVDD
CCCCCCCCCCEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCH
AKAFVAAQEVRRIEHKGVALALERTTDGVLVGVMGFGLERDGTPELGYWIGKDFWGQGYA
HHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHCHHHCCCCHH
TEAANRLIRHLFDTLGFERVWAQFHPDNTGSKRVLEKVGLTFHRVDACPMPARGESVISP
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
VFQLRRAEWLMARSLRPTVLVVAAALVDGDGRVLMASRPTGKSMEGLWEFPGGKIHDGET
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCEECCCCC
PEAALVRELEEELGIDVRESCLAPVAFASHDYDTFHLLMPLYLVRVWKGNPSAREGQELR
CHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
WIRVPRLGDLPMPPADIPLVAILREWV
EEECCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8244038 [H]