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The map label for this gene is intB [H]

Identifier: 83309587

GI number: 83309587

Start: 519546

End: 520784

Strand: Reverse

Name: intB [H]

Synonym: amb0488

Alternate gene names: 83309587

Gene position: 520784-519546 (Counterclockwise)

Preceding gene: 83309588

Following gene: 83309586

Centisome position: 10.48

GC content: 59.16

Gene sequence:

>1239_bases
ATGCCCCTTACGGACTTGCAAATTAAGGCGGCGAAACCCGCCGATAAGCCAATAAAGCTGGCGGATGGCGGTGGCATGTT
CTTGCTGGTCACCCCGCAAGATCAACGCTATTGGCGTCTGAAATATCGTTTTGGCGGAAAGGAAAAACTGCTCGCGTTGG
GCGTTTACCCCGACGTGACGATCAAGACCGCTCGCCAGCTTCGAGATGACGCCAAGAAACAGCTAGCCAACGGCATCGAC
CCGTCCGATGCGCGGAAGGCGAAAAAGTCATCGCAGTTGCTTGATGGCCCCGACAGTTTCGAAAACGTAGCCCGCGAATG
GTTCGCTAAGAATGTGGCCACATGGGCCGAGACGCATTCATCCAAGGTTCTCCAGCGGCTTGAAAGCGATGTCTTTCCGC
TAATCGGCACCAAGCCAATCGGTTCGATTACAGCGCCGATGATCCTCGCCGTTGCCCGAAAGATTGAAGCCAGGGGGGCT
AACGATACGGCCCATAGGGCAGTTCAGAACGCAGGCCAAATTATGCGTTACGCTGTCGCCACTGGGCGCGCCGAACGCGA
CCCCACCGGCGACTTGCGCGGGGCCTTACCGCCTGTCCGCCACAAACACTTCGCCGCCCTTACCGACCCGAAGCTGGTTG
GTGAGTTGCTCCGCGCATTCGACGCCTTTGAGGGCACCGTCGTCGTCAAGGCAGCGCTACAACTTGCCCCTCTGGTTTTC
GTCCGCCCTGGTGAACTTAGAAAGGCCAAGTGGGCAGACATCGACCTTGATGCTGGAAGCTGGGCCTTCCATGCCTCCAA
GGCCCGAAAGGGCGAAACGATTGAACATCTGGTCCCGTTATCAACCCAGGCCGTTGAAATTCTGCGCGACTTGCACCAGT
ACACCGGCAACCGTGAATGCTTGTTTCCCGGCCGCGATCCGCGAAAGCCGATGAGTGAGGCCGCTGTAAACGCCGCATTG
CGCCGTCTTGGCTACGACACCAAGACCGAGATTACGGGCCACGGCTTCCGAGCGATGGCCCGCACGATCTTGGCCGAGCA
GCTTGGGGAAGACCCACACGTTATTGAGCAGCAGCTTGCCCACCGCGTGCCGGACGCCCTTGGCCGAGCATACAACCGCA
CCAAGTACATCGAACAGCGACGTGCGATGATGCAGCGCTGGTCGGACTACCTGGACACGCTCAAGGCTGGAGCTGAGGTA
ATTCAGCTTCCCCAGGCGAGGGGGCGGCATGCCAACTGA

Upstream 100 bases:

>100_bases
AATCCGGCCGTCCGCGTTCGTATCAAATCGCTGGAAGCCGGGGGTACATGGGGGTATATTTGGGGGTAGATATGTCCATC
CGCGATGGAGATACCCCCAA

Downstream 100 bases:

>100_bases
CGATCCCGATAGTTTGGAGGCAATCCCACCTGATCTCATTGAACGTATGCGTGCGGTCTGCACCGAGTCGGTTCAGCTAC
TCGGAGTCGATGGCGCTGAA

Product: integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPLTDLQIKAAKPADKPIKLADGGGMFLLVTPQDQRYWRLKYRFGGKEKLLALGVYPDVTIKTARQLRDDAKKQLANGID
PSDARKAKKSSQLLDGPDSFENVAREWFAKNVATWAETHSSKVLQRLESDVFPLIGTKPIGSITAPMILAVARKIEARGA
NDTAHRAVQNAGQIMRYAVATGRAERDPTGDLRGALPPVRHKHFAALTDPKLVGELLRAFDAFEGTVVVKAALQLAPLVF
VRPGELRKAKWADIDLDAGSWAFHASKARKGETIEHLVPLSTQAVEILRDLHQYTGNRECLFPGRDPRKPMSEAAVNAAL
RRLGYDTKTEITGHGFRAMARTILAEQLGEDPHVIEQQLAHRVPDALGRAYNRTKYIEQRRAMMQRWSDYLDTLKAGAEV
IQLPQARGRHAN

Sequences:

>Translated_412_residues
MPLTDLQIKAAKPADKPIKLADGGGMFLLVTPQDQRYWRLKYRFGGKEKLLALGVYPDVTIKTARQLRDDAKKQLANGID
PSDARKAKKSSQLLDGPDSFENVAREWFAKNVATWAETHSSKVLQRLESDVFPLIGTKPIGSITAPMILAVARKIEARGA
NDTAHRAVQNAGQIMRYAVATGRAERDPTGDLRGALPPVRHKHFAALTDPKLVGELLRAFDAFEGTVVVKAALQLAPLVF
VRPGELRKAKWADIDLDAGSWAFHASKARKGETIEHLVPLSTQAVEILRDLHQYTGNRECLFPGRDPRKPMSEAAVNAAL
RRLGYDTKTEITGHGFRAMARTILAEQLGEDPHVIEQQLAHRVPDALGRAYNRTKYIEQRRAMMQRWSDYLDTLKAGAEV
IQLPQARGRHAN
>Mature_411_residues
PLTDLQIKAAKPADKPIKLADGGGMFLLVTPQDQRYWRLKYRFGGKEKLLALGVYPDVTIKTARQLRDDAKKQLANGIDP
SDARKAKKSSQLLDGPDSFENVAREWFAKNVATWAETHSSKVLQRLESDVFPLIGTKPIGSITAPMILAVARKIEARGAN
DTAHRAVQNAGQIMRYAVATGRAERDPTGDLRGALPPVRHKHFAALTDPKLVGELLRAFDAFEGTVVVKAALQLAPLVFV
RPGELRKAKWADIDLDAGSWAFHASKARKGETIEHLVPLSTQAVEILRDLHQYTGNRECLFPGRDPRKPMSEAAVNAALR
RLGYDTKTEITGHGFRAMARTILAEQLGEDPHVIEQQLAHRVPDALGRAYNRTKYIEQRRAMMQRWSDYLDTLKAGAEVI
QLPQARGRHAN

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=402, Percent_Identity=39.3034825870647, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788690, Length=393, Percent_Identity=35.8778625954198, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788974, Length=403, Percent_Identity=33.0024813895782, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45747; Mature: 45616

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDLQIKAAKPADKPIKLADGGGMFLLVTPQDQRYWRLKYRFGGKEKLLALGVYPDVT
CCCCCCEEECCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCH
IKTARQLRDDAKKQLANGIDPSDARKAKKSSQLLDGPDSFENVAREWFAKNVATWAETHS
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SKVLQRLESDVFPLIGTKPIGSITAPMILAVARKIEARGANDTAHRAVQNAGQIMRYAVA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TGRAERDPTGDLRGALPPVRHKHFAALTDPKLVGELLRAFDAFEGTVVVKAALQLAPLVF
HCCCCCCCCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEE
VRPGELRKAKWADIDLDAGSWAFHASKARKGETIEHLVPLSTQAVEILRDLHQYTGNREC
ECCCCCCCCCCCCEECCCCCCHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEE
LFPGRDPRKPMSEAAVNAALRRLGYDTKTEITGHGFRAMARTILAEQLGEDPHVIEQQLA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
HRVPDALGRAYNRTKYIEQRRAMMQRWSDYLDTLKAGAEVIQLPQARGRHAN
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLTDLQIKAAKPADKPIKLADGGGMFLLVTPQDQRYWRLKYRFGGKEKLLALGVYPDVT
CCCCCEEECCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCH
IKTARQLRDDAKKQLANGIDPSDARKAKKSSQLLDGPDSFENVAREWFAKNVATWAETHS
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SKVLQRLESDVFPLIGTKPIGSITAPMILAVARKIEARGANDTAHRAVQNAGQIMRYAVA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TGRAERDPTGDLRGALPPVRHKHFAALTDPKLVGELLRAFDAFEGTVVVKAALQLAPLVF
HCCCCCCCCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEE
VRPGELRKAKWADIDLDAGSWAFHASKARKGETIEHLVPLSTQAVEILRDLHQYTGNREC
ECCCCCCCCCCCCEECCCCCCHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEE
LFPGRDPRKPMSEAAVNAALRRLGYDTKTEITGHGFRAMARTILAEQLGEDPHVIEQQLA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
HRVPDALGRAYNRTKYIEQRRAMMQRWSDYLDTLKAGAEVIQLPQARGRHAN
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]