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The map label for this gene is barA [H]

Identifier: 83309540

GI number: 83309540

Start: 485084

End: 487306

Strand: Reverse

Name: barA [H]

Synonym: amb0441

Alternate gene names: 83309540

Gene position: 487306-485084 (Counterclockwise)

Preceding gene: 83309541

Following gene: 83309539

Centisome position: 9.81

GC content: 67.75

Gene sequence:

>2223_bases
GTGACAAGCCGGGCCGGAACAGACGAGCGGGACCTTGCCGTGAGAGATGAACTCGCGCGTCTTCGCGCGGCTCTCGACAA
GGGCGAACAGGAGCGCTCGGACCTGGAGATCGAGCTTCAGGCCGCCATCGAGCATGGCGACGCCATCGAGGCGGAACTGG
CGCTGGCCAACGACCAGATGCGTGCCGAGATCGCCGAGCGCATCCGGGCCGAGGCCAAGCTGCAGCGCCTGCTTGCCGCC
CTGGGCGAGCAGAAGGCCGACCTCGAGATGCTGGTCCACACCATCACCCAGCACAGCGACGAGATCGACATCGAGCAGGA
GCTGGCCAACGAGCAGCTTCGCCTTGAGAACGAGCGCATCAACCTCGCCAAGGAGCAGGCGGAAAGCCTGGCGCAGGCCA
AGACGGAATTCGTCGCCGTGGTCTCCCACGAGGTCCGCACCCCCATGAACGGGCTGCTGGGCATGACCCAGTTGCTGCTA
GACACCCCCCTGACCCAGGAACAGCGCGACATCGCCGGTACCGTGATGTCCTCGGGCCGCCTGCTGCTCAACATCCTCGA
CGAGTTGCTGGACCTGTCCAAGATCGAGGCCGGCAAGCTGCAGATCGAGCACTTGGAGGTCAAGGTCGTCGATCTGGTGG
AGGAAAGCTTCGGCCTGATGAGCATGCGTGCCACCGAGCGCGGCCTGGCGGTAGCCCATGCCATCGCCCCGGATGTTCCG
GCCATGGTCACCGGTGATCCGTCCCGCCTGCGCCAGGTGCTGCTGAACCTGATCGGCAACGCCATCAAATTCACCGAAAA
AGGCAGCGTCACCCTGACGGTGACACGGGTGCCCGTTCACAGGACCGGCGGCGAGGGACTGCGCTTTGCCGTCGCCGATA
CCGGAATCGGCATTCCCGAGGAGGCGCGGGACCAGCTGTTCCGCCGCTACGTTCAGGCCGCCGCCTGGGTATCGCGCAAA
TTCGGCGGAACCGGCCTCGGCCTGTCCATCTGCCGGCAGCTGGTGGAACTGATGGGCGGCGAGATCGGCGTCGACAGCGT
CGCGGGACAGGGCAGCGTGTTCTGGTTCCAAATTCCGCTGATCCCCGTGGCGGCAAGCCCCGGACGTTCTCGCCCGGCCC
ATGCTCCGGCGCGGCTGCTGCTGCTGGAGCCAGAAGCACCGGTCCGCACCGCGCTGACAACCATTCTGGCCGGCTGGGGC
ATCCAGGTCATGGAGGCCACCAAGGCCAGTGCCGCGACACTGGGGCGGCCGGGCGACGTTCTGCTGCTCGGCGGACGCAT
GGCGCTTTCCGAGGCGCAGGTGTTGGCCCGGAATTCCGGGCTTGCCGCCATCATCCTGTCGCCCCTGGGAACCACGCCCA
CCACGGGGGAAACCGATGCGGTGGCAATCCTGCCCGAGCCGGTGCGCGAAGCGGCCCTGATCCGGGCTCTCGACCAGTTT
GGCGCCCCTGGACGGGCGATCGGGCCGGGCCCGGCCGATCTCCCCCACAGGGGGCAGGAGGCCGCCCAGGCAGGACAGAG
TCTCACCGTGCTGGTGGTCGAGGACAATCCGGTCAACCGGCGCGTGGCCGGCGGCTTCCTCTCCCGCCAGGGCCACACGG
TGCTGATGGCGGAAAACGGCGCCCAGGCCTTGGAGGTCATCGCGTCGCACCCCGTCGACATCATCCTCATGGATCGTCAC
ATGCCGGTGATGGACGGCCTGCAGGCGGTCCGCGCCCTGCGGGCCATGCCGCCCCCCGCCGGCCATCTGCCGGTGGTGGC
CCTGACGGCGGCGGCGACCCGCGAAGAGGCACGGGAGTGCCTGGATGCCGGCATGAACGACTTTATTTCCAAGCCGTTCA
CCCCCGAACAACTGACCGAAGCCATCGACCGCCAGATGCGGGGCCGCCTGACCGCCGATTTCGACCGCGCCGCCCTCGTC
ACCTTATCCGAAAGGATCGGCGACGATGGCCTGGTCGAGATCATCGGCGACTATCGGACCGTCTCGCAATCCCTGGCCGA
AGCCATTGTCCGGGCAGCAACCATGCGCGACGGACAAGCCCTGGGAGAAGCCGCCCACAGCCTTAGCGGATGCTCGGGAC
AACTGGGCCTGAACGGCATCTCCGGTCTTTGCCGCGCCGTGGAGGGCGCCTTGCGCGAAGGGCGTCTGGACGATGCCCTG
GCCATGGCGGCTCAGATCCCAGCAGCCCATGCCCGGGGACTGGCCTTTCTGCGCGACGGCTGA

Upstream 100 bases:

>100_bases
CCTTGATCAAGCCGTCTGTTCCTGCCATCAGTGATACGACATTAGCCGATGATAATCTTCGCCGCCGCCTGGGCGAAGAA
TCCGCGGAACGGGAGAGCGC

Downstream 100 bases:

>100_bases
CTTGTGGCCGCCGGGGAAGGATGGCACACTCCGGCCCGCGAACGGCACTTGCAACCTCCGAGGGGAGAGGGAATGGCAGA
GGTCATCGGCGATTTTTCCG

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MTSRAGTDERDLAVRDELARLRAALDKGEQERSDLEIELQAAIEHGDAIEAELALANDQMRAEIAERIRAEAKLQRLLAA
LGEQKADLEMLVHTITQHSDEIDIEQELANEQLRLENERINLAKEQAESLAQAKTEFVAVVSHEVRTPMNGLLGMTQLLL
DTPLTQEQRDIAGTVMSSGRLLLNILDELLDLSKIEAGKLQIEHLEVKVVDLVEESFGLMSMRATERGLAVAHAIAPDVP
AMVTGDPSRLRQVLLNLIGNAIKFTEKGSVTLTVTRVPVHRTGGEGLRFAVADTGIGIPEEARDQLFRRYVQAAAWVSRK
FGGTGLGLSICRQLVELMGGEIGVDSVAGQGSVFWFQIPLIPVAASPGRSRPAHAPARLLLLEPEAPVRTALTTILAGWG
IQVMEATKASAATLGRPGDVLLLGGRMALSEAQVLARNSGLAAIILSPLGTTPTTGETDAVAILPEPVREAALIRALDQF
GAPGRAIGPGPADLPHRGQEAAQAGQSLTVLVVEDNPVNRRVAGGFLSRQGHTVLMAENGAQALEVIASHPVDIILMDRH
MPVMDGLQAVRALRAMPPPAGHLPVVALTAAATREEARECLDAGMNDFISKPFTPEQLTEAIDRQMRGRLTADFDRAALV
TLSERIGDDGLVEIIGDYRTVSQSLAEAIVRAATMRDGQALGEAAHSLSGCSGQLGLNGISGLCRAVEGALREGRLDDAL
AMAAQIPAAHARGLAFLRDG

Sequences:

>Translated_740_residues
MTSRAGTDERDLAVRDELARLRAALDKGEQERSDLEIELQAAIEHGDAIEAELALANDQMRAEIAERIRAEAKLQRLLAA
LGEQKADLEMLVHTITQHSDEIDIEQELANEQLRLENERINLAKEQAESLAQAKTEFVAVVSHEVRTPMNGLLGMTQLLL
DTPLTQEQRDIAGTVMSSGRLLLNILDELLDLSKIEAGKLQIEHLEVKVVDLVEESFGLMSMRATERGLAVAHAIAPDVP
AMVTGDPSRLRQVLLNLIGNAIKFTEKGSVTLTVTRVPVHRTGGEGLRFAVADTGIGIPEEARDQLFRRYVQAAAWVSRK
FGGTGLGLSICRQLVELMGGEIGVDSVAGQGSVFWFQIPLIPVAASPGRSRPAHAPARLLLLEPEAPVRTALTTILAGWG
IQVMEATKASAATLGRPGDVLLLGGRMALSEAQVLARNSGLAAIILSPLGTTPTTGETDAVAILPEPVREAALIRALDQF
GAPGRAIGPGPADLPHRGQEAAQAGQSLTVLVVEDNPVNRRVAGGFLSRQGHTVLMAENGAQALEVIASHPVDIILMDRH
MPVMDGLQAVRALRAMPPPAGHLPVVALTAAATREEARECLDAGMNDFISKPFTPEQLTEAIDRQMRGRLTADFDRAALV
TLSERIGDDGLVEIIGDYRTVSQSLAEAIVRAATMRDGQALGEAAHSLSGCSGQLGLNGISGLCRAVEGALREGRLDDAL
AMAAQIPAAHARGLAFLRDG
>Mature_739_residues
TSRAGTDERDLAVRDELARLRAALDKGEQERSDLEIELQAAIEHGDAIEAELALANDQMRAEIAERIRAEAKLQRLLAAL
GEQKADLEMLVHTITQHSDEIDIEQELANEQLRLENERINLAKEQAESLAQAKTEFVAVVSHEVRTPMNGLLGMTQLLLD
TPLTQEQRDIAGTVMSSGRLLLNILDELLDLSKIEAGKLQIEHLEVKVVDLVEESFGLMSMRATERGLAVAHAIAPDVPA
MVTGDPSRLRQVLLNLIGNAIKFTEKGSVTLTVTRVPVHRTGGEGLRFAVADTGIGIPEEARDQLFRRYVQAAAWVSRKF
GGTGLGLSICRQLVELMGGEIGVDSVAGQGSVFWFQIPLIPVAASPGRSRPAHAPARLLLLEPEAPVRTALTTILAGWGI
QVMEATKASAATLGRPGDVLLLGGRMALSEAQVLARNSGLAAIILSPLGTTPTTGETDAVAILPEPVREAALIRALDQFG
APGRAIGPGPADLPHRGQEAAQAGQSLTVLVVEDNPVNRRVAGGFLSRQGHTVLMAENGAQALEVIASHPVDIILMDRHM
PVMDGLQAVRALRAMPPPAGHLPVVALTAAATREEARECLDAGMNDFISKPFTPEQLTEAIDRQMRGRLTADFDRAALVT
LSERIGDDGLVEIIGDYRTVSQSLAEAIVRAATMRDGQALGEAAHSLSGCSGQLGLNGISGLCRAVEGALREGRLDDALA
MAAQIPAAHARGLAFLRDG

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=657, Percent_Identity=31.9634703196347, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI145693157, Length=511, Percent_Identity=33.0724070450098, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI87081816, Length=330, Percent_Identity=36.3636363636364, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=38.3333333333333, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=33.1932773109244, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=30.5439330543933, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87082128, Length=255, Percent_Identity=30.5882352941176, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=31.5555555555556, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788393, Length=215, Percent_Identity=30.2325581395349, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786783, Length=250, Percent_Identity=26.8, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1786912, Length=259, Percent_Identity=25.0965250965251, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790300, Length=253, Percent_Identity=29.2490118577075, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=25, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=35.2941176470588, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788905, Length=182, Percent_Identity=27.4725274725275, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790861, Length=265, Percent_Identity=23.7735849056604, Blast_Score=63, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=264, Percent_Identity=27.6515151515151, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322000, Length=121, Percent_Identity=31.404958677686, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323034, Length=182, Percent_Identity=26.3736263736264, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 78916; Mature: 78784

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRAGTDERDLAVRDELARLRAALDKGEQERSDLEIELQAAIEHGDAIEAELALANDQM
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEEHHCCCCCHHHHHHHCCHHH
RAEIAERIRAEAKLQRLLAALGEQKADLEMLVHTITQHSDEIDIEQELANEQLRLENERI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHH
NLAKEQAESLAQAKTEFVAVVSHEVRTPMNGLLGMTQLLLDTPLTQEQRDIAGTVMSSGR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
LLLNILDELLDLSKIEAGKLQIEHLEVKVVDLVEESFGLMSMRATERGLAVAHAIAPDVP
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
AMVTGDPSRLRQVLLNLIGNAIKFTEKGSVTLTVTRVPVHRTGGEGLRFAVADTGIGIPE
EEECCCHHHHHHHHHHHHHHHHEEECCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCH
EARDQLFRRYVQAAAWVSRKFGGTGLGLSICRQLVELMGGEIGVDSVAGQGSVFWFQIPL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECE
IPVAASPGRSRPAHAPARLLLLEPEAPVRTALTTILAGWGIQVMEATKASAATLGRPGDV
EEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCE
LLLGGRMALSEAQVLARNSGLAAIILSPLGTTPTTGETDAVAILPEPVREAALIRALDQF
EEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
GAPGRAIGPGPADLPHRGQEAAQAGQSLTVLVVEDNPVNRRVAGGFLSRQGHTVLMAENG
CCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHHCCHHCCCCCEEEEECCC
AQALEVIASHPVDIILMDRHMPVMDGLQAVRALRAMPPPAGHLPVVALTAAATREEAREC
HHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHH
LDAGMNDFISKPFTPEQLTEAIDRQMRGRLTADFDRAALVTLSERIGDDGLVEIIGDYRT
HHCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
VSQSLAEAIVRAATMRDGQALGEAAHSLSGCSGQLGLNGISGLCRAVEGALREGRLDDAL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
AMAAQIPAAHARGLAFLRDG
HHHHHCCHHHHCCEEEEECC
>Mature Secondary Structure 
TSRAGTDERDLAVRDELARLRAALDKGEQERSDLEIELQAAIEHGDAIEAELALANDQM
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEEHHCCCCCHHHHHHHCCHHH
RAEIAERIRAEAKLQRLLAALGEQKADLEMLVHTITQHSDEIDIEQELANEQLRLENERI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHH
NLAKEQAESLAQAKTEFVAVVSHEVRTPMNGLLGMTQLLLDTPLTQEQRDIAGTVMSSGR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
LLLNILDELLDLSKIEAGKLQIEHLEVKVVDLVEESFGLMSMRATERGLAVAHAIAPDVP
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
AMVTGDPSRLRQVLLNLIGNAIKFTEKGSVTLTVTRVPVHRTGGEGLRFAVADTGIGIPE
EEECCCHHHHHHHHHHHHHHHHEEECCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCH
EARDQLFRRYVQAAAWVSRKFGGTGLGLSICRQLVELMGGEIGVDSVAGQGSVFWFQIPL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECE
IPVAASPGRSRPAHAPARLLLLEPEAPVRTALTTILAGWGIQVMEATKASAATLGRPGDV
EEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCE
LLLGGRMALSEAQVLARNSGLAAIILSPLGTTPTTGETDAVAILPEPVREAALIRALDQF
EEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
GAPGRAIGPGPADLPHRGQEAAQAGQSLTVLVVEDNPVNRRVAGGFLSRQGHTVLMAENG
CCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHHCCHHCCCCCEEEEECCC
AQALEVIASHPVDIILMDRHMPVMDGLQAVRALRAMPPPAGHLPVVALTAAATREEAREC
HHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHH
LDAGMNDFISKPFTPEQLTEAIDRQMRGRLTADFDRAALVTLSERIGDDGLVEIIGDYRT
HHCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
VSQSLAEAIVRAATMRDGQALGEAAHSLSGCSGQLGLNGISGLCRAVEGALREGRLDDAL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
AMAAQIPAAHARGLAFLRDG
HHHHHCCHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]