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The map label for this gene is gidB

Identifier: 82752288

GI number: 82752288

Start: 2737522

End: 2738241

Strand: Reverse

Name: gidB

Synonym: SAB2586c

Alternate gene names: 82752288

Gene position: 2738241-2737522 (Counterclockwise)

Preceding gene: 82752289

Following gene: 82752287

Centisome position: 99.84

GC content: 33.61

Gene sequence:

>720_bases
ATGACTGTAGAATGGTTAGCAGAACAATTAAAAGAACATAATATTGAATTAACTGAGACTCAAAAACAACAGTTTCAAAC
ATATTATTGTTTACTTGTTGAATGGAATGAAAAGATGAATTTGACAAGTATTACAGATGAACACGATGTATATTTGAAAC
ATTTTTATGATTCCATTGCACCTAGTTTTTATTTTGATTTTAATCAGCCTATAAGTATATGTGATGTAGGCGCTGGAGCT
GGTTTTCCAAGTATTCCGTTAAAAATAATGTTTCCGCAGTTAAAAGTGACGATTGTTGATTCATTAAATAAGCGTATTCA
ATTTTTAAACCATTTAGCGTCAGAATTACAATTACAGGATGTCAGCTTTATACACGATAGAGCAGAAACATTTGGTAAGG
GTGTCTACAGGGAGTCTTATGATGTTGTTACTGCAAGAGCAGTAGCTAGATTATCCGTGTTGAGTGAATTGTGTTTACCG
CTAGTTAAAAAAGGTGGACAGTTTGTTGCATTAAAATCTTCAAAAGGTGAAGAAGAATTAGAAGAAGCAAAATTTGCAAT
TAGTGTGTTAGGTGGTAACGTTACAGAAACACATACCTATGAATTGCCAGAAGATGCTGGAGAGCGCCAGATGTTCATTA
TTGATAAAAAAAGACAGACGCCGAAAAAGTATCCAAGAAAACCAGGGACGCCTAATAAGACTCCTTTACTTGAAAAATAA

Upstream 100 bases:

>100_bases
ATATTGCACAAGCTTCTAGAATATCAGGGGTAAATCCAGCAGACATATCTATATTATTGATTTACTTAGAACAAGGTAAA
CTCCAAAGGGTGAGTTACTA

Downstream 100 bases:

>100_bases
TGCATAATCCTTTACAACTAACATAAAAGGAGCGAATGGATAATGAAAAAACCTTTTTCAAAATTATTTGGTTTGAAAAA
CAAAGATGACATCATTGGAC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTVEWLAEQLKEHNIELTETQKQQFQTYYCLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGA
GFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTYELPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK

Sequences:

>Translated_239_residues
MTVEWLAEQLKEHNIELTETQKQQFQTYYCLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGA
GFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTYELPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK
>Mature_238_residues
TVEWLAEQLKEHNIELTETQKQQFQTYYCLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAG
FPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPL
VKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTYELPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=209, Percent_Identity=33.4928229665072, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27324; Mature: 27192

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEWLAEQLKEHNIELTETQKQQFQTYYCLLVEWNEKMNLTSITDEHDVYLKHFYDSIA
CCHHHHHHHHHHCCCEEEHHHHHHHHEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHC
PSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQD
CCEEECCCCCCCEEECCCCCCCCCCCEEEECCHHEEHHHHHHHHHHHHHHHHHHHHHHHH
VSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPLVKKGGQFVALKSSKGEEEL
HHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
EEAKFAISVLGGNVTETHTYELPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK
HHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVEWLAEQLKEHNIELTETQKQQFQTYYCLLVEWNEKMNLTSITDEHDVYLKHFYDSIA
CHHHHHHHHHHCCCEEEHHHHHHHHEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHC
PSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQD
CCEEECCCCCCCEEECCCCCCCCCCCEEEECCHHEEHHHHHHHHHHHHHHHHHHHHHHHH
VSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPLVKKGGQFVALKSSKGEEEL
HHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
EEAKFAISVLGGNVTETHTYELPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK
HHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA