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The map label for this gene is phoB

Identifier: 82752208

GI number: 82752208

Start: 2643348

End: 2644772

Strand: Direct

Name: phoB

Synonym: SAB2502

Alternate gene names: 82752208

Gene position: 2643348-2644772 (Clockwise)

Preceding gene: 82752194

Following gene: 82752220

Centisome position: 96.38

GC content: 35.3

Gene sequence:

>1425_bases
ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACAGTTTTTAGTTCTGTCACACC
TGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCT
TTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATG
ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCAAAACAGAATATTACTGATTC
TGCAGCTGGCGGAACAGCCTTTAGTTCTGGTCATAAGACATATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAG
TAAAGACGGTGCTTAAACGTGCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAA
CGGAAAGCATAAAGTTGATGTTTTATTAGGCGGTGGTGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAAT
TCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGATATGCAACAAAGTGCACTTAG
TAAGCTTGAGCGTAATAAAAAAGGTTTCTTTTTAATGGTCGAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATA
TTACAGGCGTTATGTCTGAAATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAGCATAAAGAT
ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAAGGATACGAATGGAATCCTCA
ACCGATTAAATCGATGAAACACTCTGGATCATATATGACAGAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTA
ATGAAGGTTATGGCTTTGAATTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGCTAAAAAGACTACTT
AAAAAAGAAAAAGACGAAAAATCCGCAGAAGTAGAAACGCAAACAAAAGCCCTACAACACGCTATTCAGAAGCCAGTAAA
TGATAAATCTTATACAGGGTGGACTTCCGATGGACATACAGGCGAGGATGTTAATACCTACGCTTATGGACCTAGATCAG
AATCATTTAGTGGTAATATCGAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA

Upstream 100 bases:

>100_bases
CTAAATCACTCTGTTTACATATTTTTAACTAAAACTACATAATTGCTCAAAATTCCTCTTTTACACTGAAGACATAGTCA
TATACTCAGGAGGATGTCTT

Downstream 100 bases:

>100_bases
AGCATAAAAAAATTCCCGAGCACTACAACAAGGTGTTCGGGAATTTTTTATGGTTTAATTTAGTACTCAATGTTAGGTGC
TTGGTTAACACGTTCTTGGT

Product: alkaline phosphatase III precursor

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEM
TPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLKRAKEKGKSTGLVSTAELTDAT
PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLF
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKD
TLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLL
KKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

Sequences:

>Translated_474_residues
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEM
TPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLKRAKEKGKSTGLVSTAELTDAT
PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLF
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKD
TLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLL
KKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK
>Mature_474_residues
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEM
TPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLKRAKEKGKSTGLVSTAELTDAT
PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLF
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKD
TLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLL
KKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=338, Percent_Identity=31.3609467455621, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI94721246, Length=482, Percent_Identity=28.0082987551867, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157266292, Length=476, Percent_Identity=27.9411764705882, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157266296, Length=482, Percent_Identity=27.8008298755187, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI294660770, Length=329, Percent_Identity=30.3951367781155, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI294660772, Length=302, Percent_Identity=29.4701986754967, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI48994877, Length=349, Percent_Identity=36.676217765043, Blast_Score=174, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6320689, Length=526, Percent_Identity=32.5095057034221, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI18859923, Length=487, Percent_Identity=29.3634496919918, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21355149, Length=476, Percent_Identity=29.6218487394958, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651554, Length=494, Percent_Identity=29.1497975708502, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921912, Length=473, Percent_Identity=27.906976744186, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355981, Length=473, Percent_Identity=27.906976744186, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI85725200, Length=477, Percent_Identity=26.8343815513627, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI85815833, Length=477, Percent_Identity=26.8343815513627, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21355151, Length=337, Percent_Identity=30.2670623145401, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24651556, Length=481, Percent_Identity=28.6902286902287, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585213, Length=348, Percent_Identity=31.3218390804598, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21358067, Length=339, Percent_Identity=27.7286135693215, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24657835, Length=478, Percent_Identity=26.1506276150628, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24657842, Length=339, Percent_Identity=30.9734513274336, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24657827, Length=467, Percent_Identity=27.1948608137045, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51960; Mature: 51960

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGM
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCEECCCCCCCCEEEEEEECCC
GPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKT
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YNGAIGVDSNKQKVKTVLKRAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCHHHHHHHCCCCCHHHHHHH
FYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLF
HHHHHCCCCEEEEEEECCCHHCCCCCCCCCHHHHHCCCCCCCCCCHHHCCCCHHHEEEEE
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSE
ECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHH
MEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHCCCHHH
EKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLLKKEKDEKSAEVETQTKALQH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK
HHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGM
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCEECCCCCCCCEEEEEEECCC
GPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKT
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YNGAIGVDSNKQKVKTVLKRAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCHHHHHHHCCCCCHHHHHHH
FYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLF
HHHHHCCCCEEEEEEECCCHHCCCCCCCCCHHHHHCCCCCCCCCCHHHCCCCHHHEEEEE
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSE
ECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHH
MEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHCCCHHH
EKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLLKKEKDEKSAEVETQTKALQH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK
HHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]