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The map label for this gene is lacA [C]

Identifier: 82752137

GI number: 82752137

Start: 2568722

End: 2569303

Strand: Reverse

Name: lacA [C]

Synonym: SAB2429c

Alternate gene names: 82752137

Gene position: 2569303-2568722 (Counterclockwise)

Preceding gene: 82752140

Following gene: 82752136

Centisome position: 93.68

GC content: 36.08

Gene sequence:

>582_bases
ATGTTAGCAGAAAAATGGTATGATGCAAACTTTGATCAAGACTTAATCAATGAACGTGCACGAGCGAAAGATATTTGCTT
TGAATTAAATCATACAAGACCGAGTGACACAAATAAAAGAAAGGAATTAATCGATCAATTATTTCAAACAACAACGGACA
ATGTAAGTATTTCGATTCCTTTTGATACTGATTATGGATGGAACGTAAAACTTGGGAAAAATGTCTTTGTAAATACCAAT
TGTTATTTTATGGATGGTGGACAGATTACAATTGGCGATAATGTTTTTATAGGACCTAATTGTGGATTTTACACAGCGAC
ACATCCACTTAATTTTCATCATAGAAATGAAGGTTTTGAAAAAGCCGGACCCATTCATATTGGCAGTAATACTTGGTTTG
GCGGTCATGTAGTCGTGTTACCAGGGGTGACGATTGGAGAAGGTAGTGTGATTGGTGCAGGTAGTGTTGTCACAAAAGAT
ATTCCGCCACACAGTTTAGCGGTTGGAAATCCTTGTAAAGTCGTTCGTAAAATTGATAATGAGGTACCATCAGAAGCATT
GAACGATGAAACACTATATTAG

Upstream 100 bases:

>100_bases
TATAGCGAATTAGTTGCATAGGCGAATGTTTATTTGATTAACTTTTAAACAAGTAAATAAGTTATTAGGAGTTGAAAGGT
GTAATGACTGAAAAAGAAAA

Downstream 100 bases:

>100_bases
TTGTCATGACACTTCTAAATTTTAAAAAGTAACTAATCATTTTCAAAATGCTTTAGAATAAGAGATGAAGCATAAAAATG
AAAGCGATTGCAAAACCGAC

Product: galactoside-O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MLAEKWYDANFDQDLINERARAKDICFELNHTRPSDTNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVFVNTN
CYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVVVLPGVTIGEGSVIGAGSVVTKD
IPPHSLAVGNPCKVVRKIDNEVPSEALNDETLY

Sequences:

>Translated_193_residues
MLAEKWYDANFDQDLINERARAKDICFELNHTRPSDTNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVFVNTN
CYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVVVLPGVTIGEGSVIGAGSVVTKD
IPPHSLAVGNPCKVVRKIDNEVPSEALNDETLY
>Mature_193_residues
MLAEKWYDANFDQDLINERARAKDICFELNHTRPSDTNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVFVNTN
CYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVVVLPGVTIGEGSVIGAGSVVTKD
IPPHSLAVGNPCKVVRKIDNEVPSEALNDETLY

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=169, Percent_Identity=44.3786982248521, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI1786664, Length=178, Percent_Identity=38.7640449438202, Blast_Score=139, Evalue=9e-35,
Organism=Escherichia coli, GI1788367, Length=151, Percent_Identity=32.4503311258278, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322243, Length=174, Percent_Identity=49.4252873563218, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21385; Mature: 21385

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEKWYDANFDQDLINERARAKDICFELNHTRPSDTNKRKELIDQLFQTTTDNVSISIP
CCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
FDTDYGWNVKLGKNVFVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFE
CCCCCCCEEEECCEEEECCCEEEEECCEEEECCEEEECCCCCCEEECCCCCCCCCCCCCC
KAGPIHIGSNTWFGGHVVVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKIDN
CCCCEEECCCCCCCCEEEEECCEEECCCCEEECCCEEECCCCCCCEECCCHHHHHHHHHH
EVPSEALNDETLY
HCCHHHCCCCCCC
>Mature Secondary Structure
MLAEKWYDANFDQDLINERARAKDICFELNHTRPSDTNKRKELIDQLFQTTTDNVSISIP
CCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
FDTDYGWNVKLGKNVFVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFE
CCCCCCCEEEECCEEEECCCEEEEECCEEEECCEEEECCCCCCEEECCCCCCCCCCCCCC
KAGPIHIGSNTWFGGHVVVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKIDN
CCCCEEECCCCCCCCEEEEECCEEECCCCEEECCCEEECCCCCCCEECCCHHHHHHHHHH
EVPSEALNDETLY
HCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]