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The map label for this gene is bsp6IM [H]

Identifier: 82752078

GI number: 82752078

Start: 2501562

End: 2502770

Strand: Reverse

Name: bsp6IM [H]

Synonym: SAB2369c

Alternate gene names: 82752078

Gene position: 2502770-2501562 (Counterclockwise)

Preceding gene: 82752079

Following gene: 82752077

Centisome position: 91.26

GC content: 31.02

Gene sequence:

>1209_bases
ATGACTGATTCTGTAAGTTCTAATGATGCCTCTAAGTATTCTCTTGCAGCATTTTTTAGCGGTGTCGGAGGAATTGAGTT
AGGTTTTCATCAAACTAACAAGTTCAGAGTGGTGTACGCCAATGAATTTGATAAAAATGCACAAATAACATATGAAAATA
ATTTCAATAATGTAAGTTTAGATTGCAGAGATATCCATGATGTTAAAACGAGTGAAATTCCTATGTCTGATGTTATAGTA
GGTGGGTTTCCATGCCAGGCATTTAGCATTGCTGGATATCAAAAGGGATTTGAAGATGAAAGAGGAGATTTATTTTTTGA
GTTGTTAAGGTTTATTAATGAGAAGTCACCACAAGCAATATTTATTGAGAATGTAAAAAATATGGTCACACATGATCATG
GAAACACATTTAAAGTAATAAAAGAAGCTTTAACTTTGAATGGATATTTTATTAAGTGGAAAGTACTTAATGCTAAAGAT
TACGGGGACATACCTCAAAATAGAGAACGCATATATATTGTTGGGTTTAAGTGTCGAAAGGCTTTTGATTTATTCGAATT
CCCTGAAAAGATAGATTTAAAGACAGGCATAAAAGATATTATAAACTTCGAAAGTGAAATAGATGAACGTTATTATTATA
GGCTGAATAAGCAACCTTTTTATGACGAACTTGAAAAAGAAATGAAGGACAGTAATCTAATTTATCAATGGAGAAGAAGG
TATGTTAGAGCTAATAAATCTGGAGTAGTCCCAACTTTGACAGCAAACATGGGGACTGGTGGGCATAATGTACCATTAAT
CAAATCTAAATTTGGAATAAGAAAGTTAACGCCACGAGAGACATTTTCAGTCCAAGGATTCCCTGAAAGCTTCAAGTTAC
CTGATAACATGTCTAATGGTCAATTATATAAACAAGCAGGTAATAGTGTAGTGGTACCTGTTGTTAAAAGGATTGCAGAA
AATATAGCTGAATCATTAGATAATAGTGAGTTGCCGGTGTTTAAACTGGATATTAAATCTAAATATGTTCTTTTATATAC
TAAAATGATTGGTAGATTTGAAGGAGAAAGCTATATTTTAAATGGCTTTGATAGTGAAAACGATCTTAATAAATTTATAA
GAAAACATAAAATCAACTTTATAACTGAAGAAGAATATAAAAAAGTAATGAAAAGTAATGTAGGTAAAGAATATTATACT
CTGAAATAA

Upstream 100 bases:

>100_bases
AAATGATGTAATTGGTTGGGTTGGGAAATAGAACATATGTACGTATTCGAGATAGTGTGGTATAATCTTGAAATAACTTA
ATGAAAGTAGGTAGATGTAC

Downstream 100 bases:

>100_bases
ATTTCCCTCTCTGTCAAATCAGACTAATACATTCAATAATGGAAGTTAGGCAAACAAAAATTGCTTAACTTTCTTTTTGC
TTTTGGGAGTATAAATTTTT

Product: DNA modification methylase

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDSVSSNDASKYSLAAFFSGVGGIELGFHQTNKFRVVYANEFDKNAQITYENNFNNVSLDCRDIHDVKTSEIPMSDVIV
GGFPCQAFSIAGYQKGFEDERGDLFFELLRFINEKSPQAIFIENVKNMVTHDHGNTFKVIKEALTLNGYFIKWKVLNAKD
YGDIPQNRERIYIVGFKCRKAFDLFEFPEKIDLKTGIKDIINFESEIDERYYYRLNKQPFYDELEKEMKDSNLIYQWRRR
YVRANKSGVVPTLTANMGTGGHNVPLIKSKFGIRKLTPRETFSVQGFPESFKLPDNMSNGQLYKQAGNSVVVPVVKRIAE
NIAESLDNSELPVFKLDIKSKYVLLYTKMIGRFEGESYILNGFDSENDLNKFIRKHKINFITEEEYKKVMKSNVGKEYYT
LK

Sequences:

>Translated_402_residues
MTDSVSSNDASKYSLAAFFSGVGGIELGFHQTNKFRVVYANEFDKNAQITYENNFNNVSLDCRDIHDVKTSEIPMSDVIV
GGFPCQAFSIAGYQKGFEDERGDLFFELLRFINEKSPQAIFIENVKNMVTHDHGNTFKVIKEALTLNGYFIKWKVLNAKD
YGDIPQNRERIYIVGFKCRKAFDLFEFPEKIDLKTGIKDIINFESEIDERYYYRLNKQPFYDELEKEMKDSNLIYQWRRR
YVRANKSGVVPTLTANMGTGGHNVPLIKSKFGIRKLTPRETFSVQGFPESFKLPDNMSNGQLYKQAGNSVVVPVVKRIAE
NIAESLDNSELPVFKLDIKSKYVLLYTKMIGRFEGESYILNGFDSENDLNKFIRKHKINFITEEEYKKVMKSNVGKEYYT
LK
>Mature_401_residues
TDSVSSNDASKYSLAAFFSGVGGIELGFHQTNKFRVVYANEFDKNAQITYENNFNNVSLDCRDIHDVKTSEIPMSDVIVG
GFPCQAFSIAGYQKGFEDERGDLFFELLRFINEKSPQAIFIENVKNMVTHDHGNTFKVIKEALTLNGYFIKWKVLNAKDY
GDIPQNRERIYIVGFKCRKAFDLFEFPEKIDLKTGIKDIINFESEIDERYYYRLNKQPFYDELEKEMKDSNLIYQWRRRY
VRANKSGVVPTLTANMGTGGHNVPLIKSKFGIRKLTPRETFSVQGFPESFKLPDNMSNGQLYKQAGNSVVVPVVKRIAEN
IAESLDNSELPVFKLDIKSKYVLLYTKMIGRFEGESYILNGFDSENDLNKFIRKHKINFITEEEYKKVMKSNVGKEYYTL
K

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=362, Percent_Identity=29.8342541436464, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 46462; Mature: 46331

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSVSSNDASKYSLAAFFSGVGGIELGFHQTNKFRVVYANEFDKNAQITYENNFNNVSL
CCCCCCCCCCCHHHHHHHHCCCCCEEECEECCCCEEEEEECCCCCCCEEEEECCCCEEEE
DCRDIHDVKTSEIPMSDVIVGGFPCQAFSIAGYQKGFEDERGDLFFELLRFINEKSPQAI
EHHHCCCCCCCCCCHHHEEECCCCCCCEEECHHHCCCCCCHHHHHHHHHHHHCCCCCCEE
FIENVKNMVTHDHGNTFKVIKEALTLNGYFIKWKVLNAKDYGDIPQNRERIYIVGFKCRK
EHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEHHH
AFDLFEFPEKIDLKTGIKDIINFESEIDERYYYRLNKQPFYDELEKEMKDSNLIYQWRRR
HHHHHHCCCHHCHHHHHHHHHCCHHHHCCHHEEEECCCCCHHHHHHHHCCCHHHHHHHHH
YVRANKSGVVPTLTANMGTGGHNVPLIKSKFGIRKLTPRETFSVQGFPESFKLPDNMSNG
HHHCCCCCCCEEEEECCCCCCCCCCEEECCCCCEECCCCCCEEECCCCCCCCCCCCCCCC
QLYKQAGNSVVVPVVKRIAENIAESLDNSELPVFKLDIKSKYVLLYTKMIGRFEGESYIL
CHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEHHHHCCCCCEEEE
NGFDSENDLNKFIRKHKINFITEEEYKKVMKSNVGKEYYTLK
ECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TDSVSSNDASKYSLAAFFSGVGGIELGFHQTNKFRVVYANEFDKNAQITYENNFNNVSL
CCCCCCCCCCHHHHHHHHCCCCCEEECEECCCCEEEEEECCCCCCCEEEEECCCCEEEE
DCRDIHDVKTSEIPMSDVIVGGFPCQAFSIAGYQKGFEDERGDLFFELLRFINEKSPQAI
EHHHCCCCCCCCCCHHHEEECCCCCCCEEECHHHCCCCCCHHHHHHHHHHHHCCCCCCEE
FIENVKNMVTHDHGNTFKVIKEALTLNGYFIKWKVLNAKDYGDIPQNRERIYIVGFKCRK
EHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEHHH
AFDLFEFPEKIDLKTGIKDIINFESEIDERYYYRLNKQPFYDELEKEMKDSNLIYQWRRR
HHHHHHCCCHHCHHHHHHHHHCCHHHHCCHHEEEECCCCCHHHHHHHHCCCHHHHHHHHH
YVRANKSGVVPTLTANMGTGGHNVPLIKSKFGIRKLTPRETFSVQGFPESFKLPDNMSNG
HHHCCCCCCCEEEEECCCCCCCCCCEEECCCCCEECCCCCCEEECCCCCCCCCCCCCCCC
QLYKQAGNSVVVPVVKRIAENIAESLDNSELPVFKLDIKSKYVLLYTKMIGRFEGESYIL
CHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEHHHHCCCCCEEEE
NGFDSENDLNKFIRKHKINFITEEEYKKVMKSNVGKEYYTLK
ECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]