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The map label for this gene is pbuE [H]

Identifier: 82752047

GI number: 82752047

Start: 2470371

End: 2471555

Strand: Reverse

Name: pbuE [H]

Synonym: SAB2334c

Alternate gene names: 82752047

Gene position: 2471555-2470371 (Counterclockwise)

Preceding gene: 82752048

Following gene: 82752044

Centisome position: 90.12

GC content: 35.27

Gene sequence:

>1185_bases
ATGTCAATCATGCGACTATTTACATTCATTTTAAGTATTTTCATCGTAGGAATGGTTGAAATGATGGTTGCAGGAATTAT
GAACTTGATGAGTCAGGACTTACATGTATCGGAAGCTGTCGTTGGTCAATTAGTGACAATGTACGCTTTAACATTTGCGA
TATGTGGACCTATTCTGGTTAAATTAACGAATCGTTTTTCATCAAGGCCTGTATTATTATGGACATTACTTATATTTATC
ATTGGTAATGGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATTATCTCATCTGCAGCAGCACT
AATTATCGTAAAAGTATTAGCTATTACAGCGATGTTATCAGCACCTAAAAATCGTGGCAAAATGATTGGACTTGTCTATA
CAGGGTTTAGTGGTGCTAATGTTTTTGGTGTACCAATTGGAACGGTTATCGGCGATTTAGTAGGTTGGCGCTATACATTT
CTATTCTTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGGTGATCTATTTACCGAAGGATCAGGAAATACAACGAGG
CCCTGTGAATCATGAGGCACCATCTCATGAAAATCATGTTACTTCGAAAATATTAAGACCTGCTGAAGTAGCAAAATATT
TAATTATTACGTTTTTAGTATTGATTGCAAACTCAGTGACATTCGTCTTTATAAATCCACTTATTTTATCTAATGGTCAT
GATATGTCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGAACATCATTAGGTGGTATATTCTC
CGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCGGTTTCTATTTTTATCGTCATGATGTTACTTATGAATTTAATCT
TACCTGGTTCAGGTCTATTGTTAGCAGGACTATTTATTTGGAATATCATGCAATGGAGTACTAATCCAGCAGTGCAAAGC
GGTGTGATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCTAGTTTAAACGCTGGTATTGGTGT
TGGTGGCATTATTGGAGGCTTGGTCATGACACATGTTTCTGTTCAAGCTATCACGTATACGAGTGCCATCATTGGCGCAT
TAGGATTAATCGTTGTTTTCACATTGAAAAATAATCATTATGCTAAAACATTTAAATCATCATAA

Upstream 100 bases:

>100_bases
TAGAACGATTATATATAAAATTGTGTTGCACACATTTTTTAATAAATCGTTATTCTAAGGGAAATGAATATCGGAAATTT
TGTTTGAAAGGAGTTTTAAA

Downstream 100 bases:

>100_bases
TTCTCATATGAAAAGCACGCCTGCTATCAAATTCAGGTGTGCTTTTTTAGATGCGATAACGTTATTGATATGTGCGATAA
TAGCGACGTTCATTATGATA

Product: membrane transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFI
IGNGIIAVAPNFSILVVGRIISSAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTF
LFLIIVSIIVGFLMVIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGH
DMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQS
GVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Sequences:

>Translated_394_residues
MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFI
IGNGIIAVAPNFSILVVGRIISSAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTF
LFLIIVSIIVGFLMVIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGH
DMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQS
GVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
>Mature_393_residues
SIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFII
GNGIIAVAPNFSILVVGRIISSAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFL
FLIIVSIIVGFLMVIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHD
MSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSG
VIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786595, Length=357, Percent_Identity=22.96918767507, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787947, Length=266, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI145693198, Length=250, Percent_Identity=26.4, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787808, Length=377, Percent_Identity=26.525198938992, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=312, Percent_Identity=22.1153846153846, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42443; Mature: 42312

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAALIIVKVLAITAMLS
HHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
APKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMVIYLPK
CCCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGH
CCCHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCC
DMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLL
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVS
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHH
VQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
HHHHHHHHHHHHHHHHHHEEEECCCCCCHHCCCC
>Mature Secondary Structure 
SIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAALIIVKVLAITAMLS
HHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
APKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMVIYLPK
CCCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGH
CCCHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCC
DMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLL
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVS
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHH
VQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
HHHHHHHHHHHHHHHHHHEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]