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The map label for this gene is nasD

Identifier: 82751996

GI number: 82751996

Start: 2410620

End: 2413025

Strand: Reverse

Name: nasD

Synonym: SAB2280c

Alternate gene names: 82751996

Gene position: 2413025-2410620 (Counterclockwise)

Preceding gene: 82751997

Following gene: 82751995

Centisome position: 87.99

GC content: 36.2

Gene sequence:

>2406_bases
ATGGCAAAGCAAAAACTAGTAATGATTGGTAACGGTATGGCGGGCATTCGAACAATCGAAGAAATATTAGAGCGCGCTAA
CGATTTATATGATATTAAGGTAATTGGTAAAGAACCTTATCCAAACTATAACCGCATCATGCTTTCAAATATTTTACAAA
ATAAAATGACAGTTGAAGAAACAATTATGAATCCATATGAGTGGTATGAGGAACATGGTATCGAGTTAATTACAAATGAT
CCTGTAATTGAAGTAGATAGAGCAAATCAATCAGTTACTACTGCAAATGGTATTGAAGTATCATACGATAAATTGATTTT
TGCGACAGGTTCTAAAGCATTCGTCATTCCAGTTCCAGGTTCGACATTACCGAGTGTAATTGGTTGGAGAACGATTGATG
ATACTGAACAAATGATGAACATTGCTAAGACTAAAAAGAAAGCAATTGTAATTGGTGGAGGCTTACTAGGTTTAGAGTGT
GCACGTGGTTTATTAGATCAAGGTATGGAAGTAACGGTGTTACATTTAGCTGAATGGTTGATGGAAATGCAGCTAGACCG
TAAAGCTGGAAATATGCTTAAAGCAGATCTAGAAAAGCAAGGTATGAAGTTTGAAATGCAAGCTAACACAACTGAAATCT
TAGGAGAAGATGATGTTGAAGGTGTTAAATTGGCTGATGGACGCGAGATTCCGGCAGACTTAGTTGTTATGGCAGTAGGT
ATTCGACCATACACAGAAGTAGCCAAAGAATCAGGTCTAGATGTTAATCGTGGTATTGTTGTCAATGATGTGATGCAAAC
AAGTGATAGCAATGTATATGCAGTCGGTGAATGTGCAGAACATAACGGCAAAGTTTATGGACTCGTTGCACCACTATATG
AACAAGGTAAAGTATTAGCTGATCATTTAACAAATAAAGAAACGAACGGATACAAGGGATCAACAACATTTACGTCATTA
AAAGTTTCTGGGTGTGACTTGTATAGCGCTGGTCAAATTGTAGAAAATGCAGAAATTAAAGGTATTGAAATATTTAATAG
TGTTGATAATAACTATAAAAAAATCTTTTTAAAAGACGGTAATGTAGTTGGTGCAGTACTATATGGTGATATCGATGATG
GTTCACGCTTTTATAACATGATGAAAAAAGGTGAATCTACTGAAGATTACACACTGGTATCATTGCTTACTAAAGGTGGA
GAAGAGGCATCGCTATCAATTGCTGATATGGCTGATGATGAAACAATTTGTGGTTGTAATGGTGTTGATAAAGGCACTAT
AGTAAATGCGATTAAGGAAAATGGCTTTACAACAGTTGAAGAAGTAACGGCTAAAACAAAAGCGGGGAATTCATGTGGTA
AATGTAAACCGCAAATTGCTCAAATATTGCAGCACACTTTAGGAGATGACTTTGTTGCCGCAAAACCTGCTGGTATATGC
GGTTGTACTGATTTGACACGCGATCAAATTGTAACGCAAATAAGAGCGAAAGGTTTAAAAACATCTAAAGAAGTTCGACA
TGTTTTAAACTTTAAAAATAAAGGTGGATGTCCAAAATGTCGACCAGCAATCAACTATTATTTAAACATGGTTTATCCAC
ATGATCATGAAGATGAAAGAGAATCAAGATTTGCTAACGAACGTTACCATGCGAATATTCAAAATGATGGTACATTTTCT
GTTATACCTCAAATGCGTGGAGGTGTTACAGATGCAGACCAACTGATTCGTCTAGGAGAAGTGGCTAAGAAATATCATGT
ACCATTAGTTAAAGTGACAGGTTCACAACGTGTTGGTTTGTATGGAGTTAAAAAAGAAGAATTACCAAATATATGGGAAG
ACCTAGGTATGCGTTCAGCATCAGCTTATGGTAAGAAAACACGCTCAGTTAAAAGCTGTGTTGGTAAAGAGTTTTGTCGA
TTTGGTACGCAATACACGACACGACTTGGCATTCGTTTAGAAAAAACATTTGAATACATCGATACACCTCATAAATTCAA
AATGGGTGTATCAGGCTGCCCAAGAAGTTGTGTTGAATCAGGTGTTAAAGATTTTGGTATTATCTCAGTTGAAAATGGGT
TCCAAATCTATATCGGTGGTAATGGAGGTACAGAAGTTGAAAAAGCTGAATTTTTAACAACTGTAGAAACAGAAGATGAA
GTAATCAAATTATGTGGTGCTTTGATGCAATATTATCGCGAAACAGGTATATATGCTGAAAGAACAGCGCCATGGTTAAG
AAGACTTGGATTTGAAAATGTAAAAGAAGTCTTACTTAACCCAGAAAGACAAAATGAACTATTTGAACGCATTATGGATG
CTAAAAAAGCAGTCGAAGCTGAACCTTGGGAAGCAATAACTAGCAATGCGCAAGCACGTAAAATTTTTGAAGTGGAGAAG
GTGTAA

Upstream 100 bases:

>100_bases
TAGATTAGAAAGTCTTAATGACTCCAATGAAAATATAAAAGCACAAACGAAAATTAAAACCTTTTTGAAATAAATATTAT
GAAGTAAAGGGGCATATTTT

Downstream 100 bases:

>100_bases
TACATGGAAACAAAAGAAAAAATTAAAGTGACGACTATAGATGAATTAACACCCCTAATTGGAAAAAAGGTTATTGTCAA
AGGCAAAGAGGTAGGATTGT

Product: nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 800

Protein sequence:

>801_residues
MAKQKLVMIGNGMAGIRTIEEILERANDLYDIKVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITND
PVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSL
KVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGG
EEASLSIADMADDETICGCNGVDKGTIVNAIKENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFS
VIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCR
FGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLNPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEVEK
V

Sequences:

>Translated_801_residues
MAKQKLVMIGNGMAGIRTIEEILERANDLYDIKVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITND
PVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSL
KVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGG
EEASLSIADMADDETICGCNGVDKGTIVNAIKENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFS
VIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCR
FGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLNPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEVEK
V
>Mature_800_residues
AKQKLVMIGNGMAGIRTIEEILERANDLYDIKVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDP
VIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLECA
RGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVGI
RPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLK
VSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGE
EASLSIADMADDETICGCNGVDKGTIVNAIKENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGICG
CTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSV
IPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRF
GTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEV
IKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLNPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEVEKV

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=387, Percent_Identity=27.1317829457364, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI21389617, Length=387, Percent_Identity=27.1317829457364, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI226437568, Length=387, Percent_Identity=27.1317829457364, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI22202629, Length=221, Percent_Identity=28.9592760180996, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI4757732, Length=221, Percent_Identity=28.9592760180996, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1789765, Length=819, Percent_Identity=35.2869352869353, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789065, Length=332, Percent_Identity=27.710843373494, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788892, Length=251, Percent_Identity=23.1075697211155, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=428, Percent_Identity=27.803738317757, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32565766, Length=220, Percent_Identity=28.6363636363636, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281359715, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359713, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639250, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18543267, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639252, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639257, Length=290, Percent_Identity=27.2413793103448, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585130, Length=281, Percent_Identity=25.2669039145907, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21358499, Length=215, Percent_Identity=30.6976744186047, Blast_Score=81, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28573993, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581020, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88683; Mature: 88552

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKLVMIGNGMAGIRTIEEILERANDLYDIKVIGKEPYPNYNRIMLSNILQNKMTVEE
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
TIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPG
HHCCHHHHHHHCCCEEEECCCEEEEECCCCCEEECCCCEEEECEEEEEECCCEEEEECCC
STLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLECARGLLDQGMEVTVLHLAEWL
CCCCHHHCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEHHHHHHHHH
MEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
HHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHCCCCCCCCEEECCCCCCCHHHEEEEEC
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLA
CCCHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEEEEHHCCCCEEEEEECCHHHCCCHHH
DHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDG
HHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCCCCEEEEECCCCCCEEEEEEECC
NVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGEEASLSIADMADDETICGCN
CEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEECCC
GVDKGTIVNAIKENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
CCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDER
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHEEEECCCCCCHHH
ESRFANERYHANIQNDGTFSVIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGL
HHHHCCCCEEECCCCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYI
ECCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHEECEEEEEHHHHH
DTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
CCCCCEECCCCCCCHHHHHCCCHHCEEEEECCCEEEEECCCCCCCCHHHHHEEECCCHHH
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLNPERQNELFERIMDAKKAVEA
HHHHHHHHHHHHHHCCCCHHHCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
EPWEAITSNAQARKIFEVEKV
CCHHHHHCCCHHHHHEEECCC
>Mature Secondary Structure 
AKQKLVMIGNGMAGIRTIEEILERANDLYDIKVIGKEPYPNYNRIMLSNILQNKMTVEE
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
TIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPG
HHCCHHHHHHHCCCEEEECCCEEEEECCCCCEEECCCCEEEECEEEEEECCCEEEEECCC
STLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLECARGLLDQGMEVTVLHLAEWL
CCCCHHHCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEHHHHHHHHH
MEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
HHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHCCCCCCCCEEECCCCCCCHHHEEEEEC
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLA
CCCHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEEEEHHCCCCEEEEEECCHHHCCCHHH
DHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDG
HHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCCCCEEEEECCCCCCEEEEEEECC
NVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGEEASLSIADMADDETICGCN
CEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEECCC
GVDKGTIVNAIKENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
CCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDER
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHEEEECCCCCCHHH
ESRFANERYHANIQNDGTFSVIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGL
HHHHCCCCEEECCCCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYI
ECCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHEECEEEEEHHHHH
DTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
CCCCCEECCCCCCCHHHHHCCCHHCEEEEECCCEEEEECCCCCCCCHHHHHEEECCCHHH
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLNPERQNELFERIMDAKKAVEA
HHHHHHHHHHHHHHCCCCHHHCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
EPWEAITSNAQARKIFEVEKV
CCHHHHHCCCHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]