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The map label for this gene is yiaE [C]

Identifier: 82751894

GI number: 82751894

Start: 2308209

End: 2309162

Strand: Direct

Name: yiaE [C]

Synonym: SAB2178

Alternate gene names: 82751894

Gene position: 2308209-2309162 (Clockwise)

Preceding gene: 82751893

Following gene: 82751895

Centisome position: 84.16

GC content: 35.64

Gene sequence:

>954_bases
ATGGAAAAAGTTTATGTTGCTGGTGCAATACCAGAAGTAGGTTTAAAACTTTTACAAGAACATTTTGAAGTTGAAATGTA
TGAAGGTAAAGGATTAGTCGATAAAGACACTTTAATTAAAGGTGTTAAAAACGCGACTGCCTTAATTAGTTTATTATCTA
CAAACGTTGATAAAGATGTTATCGATGCTGGTAAAGACTTAAAAATCATTGCCAACTATGGCGCTGGTTTTAATAATATT
GATATCGAGTATGCCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAAC
AATTGGATTAGTACTTGCAATAGCGCGTCGTATTGTTGAAGGCGACCAATTATCACGTACCACTGGATTTGACGGATGGG
CACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTA
GCGCGTCGTGCAAGAGCATTTGACATGGATGTACTATACACAGGACCTAATCGCAAAGAAGAAAAAGAACGAGAAATCGG
TGCAAAATATGTAGATTTAGATACACTATTAAAGAATGCAGATTTTATCACTATCAACGCTGCTTATAATCCTAAAATGC
ATCATTTAATTGATACAGAACAATTTAAAATGATGAAATCTACGGCGTATTTAATCAATGCTTCTCGTGGTCCAATCGTG
CACGAACAAGCACTAGTGCAAGCATTGAAAGATAATGAAATTGAAGGTGCTGCACTTGATGTGTATGAATTTGAACCAGA
TATTACCGATGACTTAAAATCACTTAATAATGTAGTATTAACACCACATATTGGTAATGCTACATTTGAAGCACGTGACA
TGATGTCTAAAATTGTTGCTAATGCTGCAATTAGTGCCGTTCAAGGTGAGAAACCACAATTTGTCGTTAACTAA

Upstream 100 bases:

>100_bases
ATCAAAAGTACACGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATATTCTACTATACTAA
GATAAAAGGAGTGATCTTTT

Downstream 100 bases:

>100_bases
TAATTGAATTTATTATAATGACATTATGGTTCTATAATAAATCGGACTGATAGAAAAAGTTTTTTTACTTTTCATCTGTC
CGATTTTTTGATTTTGAATA

Product: dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNI
DIEYAREKSIDVTNTPKASTNATADLTIGLVLAIARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIV
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

Sequences:

>Translated_317_residues
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNI
DIEYAREKSIDVTNTPKASTNATADLTIGLVLAIARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIV
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN
>Mature_317_residues
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNI
DIEYAREKSIDVTNTPKASTNATADLTIGLVLAIARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIV
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=36.1904761904762, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI23308577, Length=318, Percent_Identity=32.7044025157233, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4557497, Length=260, Percent_Identity=37.6923076923077, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI61743967, Length=260, Percent_Identity=37.6923076923077, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI145580575, Length=288, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI145580578, Length=263, Percent_Identity=35.7414448669202, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4557499, Length=263, Percent_Identity=35.7414448669202, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI87082289, Length=313, Percent_Identity=36.4217252396166, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787645, Length=245, Percent_Identity=33.8775510204082, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789279, Length=281, Percent_Identity=32.0284697508897, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081824, Length=249, Percent_Identity=27.710843373494, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788660, Length=256, Percent_Identity=26.5625, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=260, Percent_Identity=33.8461538461538, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=282, Percent_Identity=28.0141843971631, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=323, Percent_Identity=33.4365325077399, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6322116, Length=303, Percent_Identity=34.983498349835, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6324055, Length=241, Percent_Identity=33.6099585062241, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324964, Length=312, Percent_Identity=23.7179487179487, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=280, Percent_Identity=24.6428571428571, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571528, Length=317, Percent_Identity=36.2776025236593, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI45552429, Length=305, Percent_Identity=34.0983606557377, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI45551003, Length=305, Percent_Identity=34.0983606557377, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI28574284, Length=305, Percent_Identity=34.0983606557377, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24585514, Length=305, Percent_Identity=34.0983606557377, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI28574282, Length=305, Percent_Identity=34.0983606557377, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI28574286, Length=313, Percent_Identity=33.8658146964856, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI19921140, Length=318, Percent_Identity=32.7044025157233, Blast_Score=151, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24646446, Length=267, Percent_Identity=32.5842696629214, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24646448, Length=267, Percent_Identity=32.5842696629214, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24646452, Length=267, Percent_Identity=32.5842696629214, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24646450, Length=267, Percent_Identity=32.5842696629214, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=32.5925925925926, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24585516, Length=304, Percent_Identity=25.9868421052632, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 34689; Mature: 34689

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDV
CCEEEEECCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
IDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAIARRIVE
HHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKE
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCH
EKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIV
HHHHHHCCEEECHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHEEEEECCCCCCH
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
NAAISAVQGEKPQFVVN
HHHHHHHCCCCCCEECC
>Mature Secondary Structure
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDV
CCEEEEECCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
IDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAIARRIVE
HHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKE
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCH
EKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIV
HHHHHHCCEEECHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHEEEEECCCCCCH
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
NAAISAVQGEKPQFVVN
HHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]