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The map label for this gene is alsS [H]

Identifier: 82751804

GI number: 82751804

Start: 2236250

End: 2237914

Strand: Reverse

Name: alsS [H]

Synonym: SAB2087c

Alternate gene names: 82751804

Gene position: 2237914-2236250 (Counterclockwise)

Preceding gene: 82751806

Following gene: 82751803

Centisome position: 81.6

GC content: 35.14

Gene sequence:

>1665_bases
ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGGGTAGAATATGTTTTTGGTAT
TCCGGGTGCAAAGATTGACTATCTATTTAATGCTTTGATTGATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAA
ACGCCGCAATGATGGCGCAAGGTATTGGAAGATTAACAGGCAAACCGGGTGTAGTACTTGTTACAAGTGGCCCTGGTGTA
AGTAATTTAACGACTGGACTATTAACAGCTACATCTGAAGGGGATCCTGTCTTAGCGTTAGGCGGCCAAGTGAAACGTAA
TGATTTATTACGATTAACGCATCAAAGTATTGATAATGCTGCTTTATTAAAATATTCATCAAAATATAGTGAAGAAGTAC
AAGATCCTGAATCATTATCAGAAGTTATGACAAATGCAATTCGAATTGCTATTTCAGGAAAAAATGGCGCAAGTTTTATT
AGTATTCCGCAAGATGTGATTTCTTCACCAGTTGAATCTAAGGCGATATCACTTTGTCAAAAACCAAATTTAGGTGTACC
AAGTGAACAGGATATTAATGATGTCATTGAAGCGATTAAAAATGCATCATTTCCGGTTTTACTAGCTGGAATGAGAAGTT
CAAGTGCAGACGAAACGAATGCTATTCGCAAATTAGTAGAGCGTACCAATTTACCCGTTGTAGAAACATTCCAAGGTGCT
GGTGTGATTAGTCGTGAATTAGAAAATCATTTCTTTGGGCGAGTGGGTTTATTCCGTAATCAAGTTGGTGATGAATTATT
ACGTAAAAGTGATTTAGTTTTTACAATCGGTTATGATCCTATAGAATATGAGGCTAGTAACTGGAATAAAGAATTAGATA
CGCAAATAATCAATATTGACGAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
GCTAAAACGATTGAAATGATTTCTGACAAAGTAGATGAACCATTTATAAATCAACAACATTTAGATGAATTAGAACAGTT
AAGAGCGCATATTGATCAAGAAACTGGTATTAAAGCAACGCATGAAGAAGGCATCTTACACCCAGTTGAAATTATAGAAT
CTATGCAAAAGGTATTAGCTGATGAAACAACAGTGACAGTTGATGTTGGTAGTCACTATATTTGGATGGCACGTAATTTC
AGAAGTTACAATCCAAGACATTTATTATTTAGTAACGGTATGCAAACGCTTGGTGTAGCATTACCGTGGGCAATTTCAGC
TGCGCTTGTACGCCCTAATACGCAAGTTGTATCCGTTGCTGGCGATGGAGGCTTTTTATTTTCATCGCAAGATTTAGAAA
CAGCCGTACGTAAAAATCTAAACATTATTCAACTTATTTGGAACGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
ATGAAATATAAACGTTCATCAGGTGTCGACTTCGGTCCTGTAGATTTCGTAAAATATGCTGAATCATTTGGCGCAAAAGG
TTTACGAGTTACTAATCAAGAAGAATTAGAAGTGGCAATTAAAGAGGGCTATGAAGCAGATGGTCCAGTATTAATTGATA
TACCTGTAAATTATAAAGATAATATCAAACTTTCAACAAATATGTTACCTAACGTATTTAACTAA

Upstream 100 bases:

>100_bases
TTTTGATAAATATCATTAATTTTACATAACTATCATTAGGTTACAATTCACAAATCAATTATATGTAATACACATCACAT
TTGAAGGGAAATGAATATAA

Downstream 100 bases:

>100_bases
AACAAAGATAAATGTTAATGAGGAGTGGGAAATTTTATGGCTAGTGTTTTATATCAACATGGTACATTAGGCACATTAAT
GGCAGGTTTATTAAAAGGAA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGV
SNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIAISGKNGASFI
SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGA
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVFTIGYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNI
AKTIEMISDKVDEPFINQQHLDELEQLRAHIDQETGIKATHEEGILHPVEIIESMQKVLADETTVTVDVGSHYIWMARNF
RSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEVAIKEGYEADGPVLIDIPVNYKDNIKLSTNMLPNVFN

Sequences:

>Translated_554_residues
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGV
SNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIAISGKNGASFI
SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGA
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVFTIGYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNI
AKTIEMISDKVDEPFINQQHLDELEQLRAHIDQETGIKATHEEGILHPVEIIESMQKVLADETTVTVDVGSHYIWMARNF
RSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEVAIKEGYEADGPVLIDIPVNYKDNIKLSTNMLPNVFN
>Mature_553_residues
TDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVS
NLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIAISGKNGASFIS
IPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGAG
VISRELENHFFGRVGLFRNQVGDELLRKSDLVFTIGYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIA
KTIEMISDKVDEPFINQQHLDELEQLRAHIDQETGIKATHEEGILHPVEIIESMQKVLADETTVTVDVGSHYIWMARNFR
SYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEM
KYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEVAIKEGYEADGPVLIDIPVNYKDNIKLSTNMLPNVFN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=27.6595744680851, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI21361361, Length=593, Percent_Identity=23.1028667790894, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=26.8248175182482, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=27.1739130434783, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=26.4814814814815, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786717, Length=574, Percent_Identity=25.4355400696864, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=24.3386243386243, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=25.4083484573503, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=25.4545454545455, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=551, Percent_Identity=22.8675136116152, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=28.2006920415225, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=24.8447204968944, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=21.7213114754098, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321524, Length=490, Percent_Identity=21.0204081632653, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=26.7482517482518, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61138; Mature: 61007

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQ
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNA
HHHHCCCCCCEEEEECCCCCCHHHHHHEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCH
ALLKYSSKYSEEVQDPESLSEVMTNAIRIAISGKNGASFISIPQDVISSPVESKAISLCQ
HHHHEHHHHHHHCCCHHHHHHHHHHEEEEEEECCCCCCEEECCHHHHCCCHHHHHHHHHC
KPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGA
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCC
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVFTIGYDPIEYEASNWNKELDTQIINID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCHHHEEECHH
EVQAEITNYMQPKKELIGNIAKTIEMISDKVDEPFINQQHLDELEQLRAHIDQETGIKAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCC
HEEGILHPVEIIESMQKVLADETTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHH
LPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
HHHHHHHHEECCCCEEEEEECCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEVAIKEGYEADGPVLIDIPVNYKD
HHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCEEEEEECCCCC
NIKLSTNMLPNVFN
CEEEECCCCCCCCC
>Mature Secondary Structure 
TDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQ
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNA
HHHHCCCCCCEEEEECCCCCCHHHHHHEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCH
ALLKYSSKYSEEVQDPESLSEVMTNAIRIAISGKNGASFISIPQDVISSPVESKAISLCQ
HHHHEHHHHHHHCCCHHHHHHHHHHEEEEEEECCCCCCEEECCHHHHCCCHHHHHHHHHC
KPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADETNAIRKLVERTNLPVVETFQGA
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCC
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVFTIGYDPIEYEASNWNKELDTQIINID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCHHHEEECHH
EVQAEITNYMQPKKELIGNIAKTIEMISDKVDEPFINQQHLDELEQLRAHIDQETGIKAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCC
HEEGILHPVEIIESMQKVLADETTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHH
LPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
HHHHHHHHEECCCCEEEEEECCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEVAIKEGYEADGPVLIDIPVNYKD
HHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCEEEEEECCCCC
NIKLSTNMLPNVFN
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]