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The map label for this gene is cobB [H]

Identifier: 82751795

GI number: 82751795

Start: 2226277

End: 2227008

Strand: Direct

Name: cobB [H]

Synonym: SAB2078

Alternate gene names: 82751795

Gene position: 2226277-2227008 (Clockwise)

Preceding gene: 82751779

Following gene: 82751799

Centisome position: 81.18

GC content: 36.75

Gene sequence:

>732_bases
TTGGAAAATGATTTAGAGACACTAAAGCATATTATAGATAGTTCGAATCGTATTACATTTTTTACAGGTGCTGGTGTATC
TGTCGCTAGTGGTGTTCCAGATTTCCGTTCAATGGGTGGCTTATTTGATGAAATTTCAAAAGATGGGCTTTCGCCAGAAT
ACTTGTTAAGCCGTGATTATTTAGAAGATGACCCTGAAGGTTTTATCAATTTCTGCCATAAGCGCTTACTATTTGTCGAT
ACGATGCCCAATATCGTTCATGATTGGATTGCAAAATTAGAACGTAATCAACAATCTTTAGGTGTCATCACGCAAAATAT
CGATGGTTTGCACTCAGACGCTGGAAGTCAACATGTTGATGAATTACATGGCACGTTAAATCGTTTTTATTGTAATGCAT
GTCATAAATCTTATACGAAGTCAGATGTCATTGATAGAACTTTGAAACATTGTGATAATTGTGGTGGCGCCATTCGACCG
GACATCGTATCATACGGTGAAATGTTAGATCAACCTACCATCATACGGGCATTAAATAAAATTGAACATGCTGACACCCT
TGTTGTACTAGGTTCATCACTCGTTGTACAACCTGCCGCAGGATTAATATCAAATTTTAAAGGCGACAATTTAATTATTA
TCAATAAAGATCGAACACCTTATGATAGCGACGCTACTTTGGTCATACATGACGATATGGTTTCCGTTGTAAAATCATTA
ATGACAGAGTAA

Upstream 100 bases:

>100_bases
GAACTGTTTAAACTGCGCCTTTGATAATCGTAAAATGAATATACTATAATAAAAGCAAAAGACGTTATAAATCATTTATT
ATTTTGATGGGGTGTTTAAT

Downstream 100 bases:

>100_bases
TCAGTACGTAAAAAGCATTTCCCGAGTAACAGGGAAATGCTTTTAATTTTGGAGTTGCGTTTTTGGAATTCGACATTTGG
AATTCGGTGTTTGGTTTTCG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVD
TMPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRP
DIVSYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSL
MTE

Sequences:

>Translated_243_residues
MENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVD
TMPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRP
DIVSYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSL
MTE
>Mature_243_residues
MENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVD
TMPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRP
DIVSYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSL
MTE

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912660, Length=213, Percent_Identity=31.924882629108, Blast_Score=107, Evalue=6e-24,
Organism=Homo sapiens, GI63054862, Length=205, Percent_Identity=32.6829268292683, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI300795542, Length=231, Percent_Identity=30.7359307359307, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7657575, Length=233, Percent_Identity=29.6137339055794, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775602, Length=218, Percent_Identity=32.5688073394495, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI13775600, Length=218, Percent_Identity=32.5688073394495, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI13787215, Length=249, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI6912664, Length=249, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI300797577, Length=258, Percent_Identity=27.1317829457364, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI300797705, Length=199, Percent_Identity=31.6582914572864, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI300797597, Length=245, Percent_Identity=26.9387755102041, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7706712, Length=263, Percent_Identity=27.7566539923954, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI215982798, Length=174, Percent_Identity=28.735632183908, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI308199517, Length=201, Percent_Identity=34.3283582089552, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17541892, Length=236, Percent_Identity=32.2033898305085, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17567771, Length=285, Percent_Identity=25.9649122807018, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990482, Length=285, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=287, Percent_Identity=26.4808362369338, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325242, Length=211, Percent_Identity=32.2274881516588, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324504, Length=275, Percent_Identity=26.5454545454545, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324599, Length=281, Percent_Identity=28.1138790035587, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320163, Length=257, Percent_Identity=25.2918287937743, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320397, Length=252, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17137536, Length=252, Percent_Identity=30.1587301587302, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648389, Length=212, Percent_Identity=32.5471698113208, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571445, Length=216, Percent_Identity=25.462962962963, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645650, Length=244, Percent_Identity=26.6393442622951, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26953; Mature: 26953

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDY
CCCHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
LEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVD
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCHHHHH
ELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRPDIVSYGEMLDQPTIIRALNK
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHH
IEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSL
HHHHHHHEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHH
MTE
HCC
>Mature Secondary Structure
MENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDY
CCCHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
LEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVD
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCHHHHH
ELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRPDIVSYGEMLDQPTIIRALNK
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHH
IEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSL
HHHHHHHEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHH
MTE
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163387 [H]