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The map label for this gene is rho

Identifier: 82751722

GI number: 82751722

Start: 2134856

End: 2136172

Strand: Reverse

Name: rho

Synonym: SAB2005c

Alternate gene names: 82751722

Gene position: 2136172-2134856 (Counterclockwise)

Preceding gene: 82751723

Following gene: 82751721

Centisome position: 77.89

GC content: 35.61

Gene sequence:

>1317_bases
ATGCCTGAAAGAGAACGTACATCTCCTCAGTATGAATCATTCCACGAATTGTACAAGAACTATACTACCAAGGAACTCAC
TCAAAAAGCTAAAACTCTTAAGTTGACGAACTATAGTAAATTAAATAAAAAAGAACTTGTTCTAGCTATTATGGAAGCAC
AAATGGAAAAAGATGGTAACTATTATATGGAAGGTATCTTAGATGATATACAACCAGATGGTTATGGTTTTTTAAGAACA
GTGAACTATTCTAAAGGGGAAAAAGATATTTATATATCTGCTAGCCAAATTCGTCGTTTTGAAATTAAACGTGGGGATAA
AGTAACTGGGAAAGTTAGAAAACCTAAAGATAACGAAAAATATTATGGCTTATTACAAGTTGACTTTGTTAATGACCATA
ACGCAGAAGAAGTGAAGAAACGTCCGCATTTCCAAGCTTTGACACCACTTTATCCAGATGAGCGTATTAAATTAGAGACA
GAAACACAAAATTATTCAACGCGCATCATGGATTTAGTAACACCGATTGGTTTAGGTCAACGTGGTTTAATAGTGGCGCC
ACCTAAAGCAGGTAAAACATCGTTATTAAAAGAGATAGCGAATGCAATCAGTACGAACAAACCAGATGCAAAGCTATTTA
TTTTGTTAGTTGGCGAGCGTCCTGAAGAGGTAACAGATTTAGAACGCTCAGTAGAAGCTGCTGAAGTCGTTCATTCAACG
TTTGACGAACCACCAGAACACCATGTTAAAGTAGCTGAATTATTACTTGAACGTGCAAAGCGTTTAGTAGAAATTGGGGA
AGATGTCATTATTTTAATGGATTCTATAACGAGATTAGCACGCGCTTATAACTTAGTTATTCCACCAAGTGGTCGTACAT
TATCAGGTGGTTTAGATCCTGCATCTTTACACAAACCAAAAGCATTCTTCGGTGCAGCGAGAAATATTGAAGCAGGTGGA
AGTTTAACAATACTTGCAACTGCATTAGTTGATACGGGTTCACGTATGGACGATATGATTTACGAAGAATTTAAAGGAAC
AGGTAACATGGAGTTACATTTAGATCGTAAATTGTCTGAACGTCGTATCTTCCCTGCAATTGATATTGGCAGAAGTTCAA
CGCGTAAAGAAGAATTGTTGATAAGTAAATCTGAATTAGACACATTATGGCAATTAAGAAATCTATTCACTGACTCAACA
GACTTTACTGAAAGATTTATTCGCAAACTTAAAAAGTCTAAGAATAATGAAGATTTCTTCAAGCAGCTACAAAAGTCTGC
AGAAGAAAGTACTAAAACGGGTCGACCTATAATTTAA

Upstream 100 bases:

>100_bases
GGGATTAGCATAATGGGATTGTGCTAGCACAGTTATTTATGCATTGTCATGCCTATCTATTACTTACTAACTAAAAAATA
ATGAAATGGGTGTAAACTAC

Downstream 100 bases:

>100_bases
TAAACATTATATAGGGGCTTGCGTTTTGAATTAATTACCTTTATAATTACACAGTATTGGGTAAAAACTCACAAATAACT
CTGTTCCAGATGGTTCAGGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRT
VNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHST
FDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGG
SLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDST
DFTERFIRKLKKSKNNEDFFKQLQKSAEESTKTGRPII

Sequences:

>Translated_438_residues
MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRT
VNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHST
FDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGG
SLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDST
DFTERFIRKLKKSKNNEDFFKQLQKSAEESTKTGRPII
>Mature_437_residues
PERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTV
NYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETE
TQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHSTF
DEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGS
LTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTD
FTERFIRKLKKSKNNEDFFKQLQKSAEESTKTGRPII

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=415, Percent_Identity=52.0481927710843, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 49931; Mature: 49800

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGN
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCC
YYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEK
EEHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
YYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETETQNYSTRIMDLVTPIGLGQ
EEEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCC
RGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHST
CCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
FDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCH
ASLHKPKAFFGAARNIEAGGSLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSE
HHCCCCHHHHHCCCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCHHH
RRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTDFTERFIRKLKKSKNNEDFF
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
KQLQKSAEESTKTGRPII
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGN
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCC
YYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEK
EEHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
YYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETETQNYSTRIMDLVTPIGLGQ
EEEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCC
RGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHST
CCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
FDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCH
ASLHKPKAFFGAARNIEAGGSLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSE
HHCCCCHHHHHCCCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCHHH
RRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTDFTERFIRKLKKSKNNEDFF
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
KQLQKSAEESTKTGRPII
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]