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The map label for this gene is cls2 [H]

Identifier: 82751690

GI number: 82751690

Start: 2107309

End: 2108793

Strand: Direct

Name: cls2 [H]

Synonym: SAB1972

Alternate gene names: 82751690

Gene position: 2107309-2108793 (Clockwise)

Preceding gene: 82751686

Following gene: 82751691

Centisome position: 76.84

GC content: 32.05

Gene sequence:

>1485_bases
ATGATAGAGTTATTATCCATTGCACTCAAGCATTCTAATATTATTTTAAATTCAATATTTATTGGTGCATTTATTTTAAA
CTTATTATTCGCCTTTACCATTATTTTCATGGAAAGACGTTCTGCCAATTCTATCTGGGCTTGGTTACTAGTCTTAGTTT
TCTTGCCTTTATTCGGCTTCATTTTATACTTACTATTAGGACGACAAATTCAACGTGACCAAATTTTCAAAATTGATAAG
GAAGATAAAAAAGGATTAGAGTTAATCGTTGATGAGCAATTAGCTGCTTTAAAAAATGAAAACTTTTCAAATTCTAATTA
TCAAATTGTAAAATTTAAAGAAATGATTCAAATGTTGTTATATAATAACGCAGCATTTTTAACAACAGACAACGATTTAA
AAATATACACAGACGGCCAAGAAAAATTTGATGACTTAATACAAGACATCCGTAATGCTACTGATTATATTCATTTTCAG
TACTATATTATTCAAAATGATGAATTAGGTCGTACCATTTTAAATGAACTTGGTAAAAAAGCGGAACAAGGTGTAGAAGT
TAAAATTCTTTATGATGACATGGGTTCTCGCGGACTGCGTAAAAAAGGTTTACGCCCGTTTCGCAATAAAGGTGGACATG
CTGAAGCATTTTTCCCATCAAAATTACCTTTAATTAACTTACGTATGAACAATCGAAACCATCGAAAAATTGTTGTAATA
GATGGGCAAATTGGATATGTTGGTGGTTTTAATATTGGTGATGAGTACTTAGGTAAATCAAAAAAATTCGGCTATTGGCG
AGATACGCATTTACGAATTGTCGGGGATGCAGTAAATGCATTGCAATTACGATTTATTCTAGATTGGAATTCACAAGCCA
CACGTGACCACATCTCCTATGATGATCGTTATTTCCCAGATGTAAATTCTGATGAAACAATTGGCGTTCAAATAGCTTCT
AGTGGTCCTGACGAAGAATGGGAACAGATTAAATATGGTTATTTGAAAATGATTTCATCTGCTAAAAAATCGATTTATAT
TCAATCTCCCTATTTCATACCTGATCAAGCCTTTTTAGATTCTATTAAAATTGCGGCATTAGGTGGTGTTGATGTCAATA
TCATGATTCCTAATAAACCTGACCATCCGTTTGTTTTTTGGGCTACTTTAAAAAATGCAGCATCCTTATTAGATGCCGGT
GTTAAAGTATTTCACTACGACAATGGCTTTTTACACTCAAAAACACTTGTTATAGATGATGAAATTGCAAGTGTGGGAAC
AGCTAATATGGACCATCGCAGTTTCACATTGAATTTCGAAGTCAACGCTTTTATTTATGACCAACAAATTGCCAAAAAAT
TAAAACAAGCTTTTATAGATGATTTAGCAGTATCTTCTGAATTAACAAAAGCACGTTATGCTAAGCGAAGTCTTTGGATT
AAATTTAAAGAAGGTATTTCACAATTATTGTCACCTATCTTATAA

Upstream 100 bases:

>100_bases
AGTGTGTTTTTCAAACTAAATTGTCATGATATTTAAATTGCTTCATGATACAATTAATAAGATATACTACTCGTTTAAGA
TTTTCGATGGGAGGTTTCAA

Downstream 100 bases:

>100_bases
AATAGAAATATGAGGAGTGTAACTTTAATGCAACAATCAGACATCATTAGTGCAGCCAAAAAATATATGGAATCTATTCA
TCAAAATGATTATACAGGCC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK
EDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNIGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSDETIGVQIAS
SGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAG
VKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL

Sequences:

>Translated_494_residues
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK
EDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNIGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSDETIGVQIAS
SGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAG
VKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL
>Mature_494_residues
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK
EDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNIGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSDETIGVQIAS
SGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAG
VKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=478, Percent_Identity=30.3347280334728, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1787007, Length=362, Percent_Identity=27.6243093922652, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56663; Mature: 56663

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50035 PLD ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGF
CHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLL
HHHHHHCCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
YNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKK
HCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHH
AEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
HHCCCEEEEEEECCCCCCHHHHCCCCHHCCCCCEEEECCCCCCEEEEEECCCCCCEEEEE
DGQIGYVGGFNIGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISY
ECCEEEEECCCCCHHHCCCCCCCCCEECCEEEEEECCCCEEEEEEEEECCCCCCCCCCCC
DDRYFPDVNSDETIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHC
SIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDD
CEEEEEECCEEEEEEECCCCCCCEEEEEEHHHHHHHHHCCEEEEEECCCEEECEEEEEEC
EIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
HHHHCCCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
KFKEGISQLLSPIL
EHHHHHHHHHHHCC
>Mature Secondary Structure
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGF
CHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLL
HHHHHHCCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
YNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKK
HCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHH
AEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
HHCCCEEEEEEECCCCCCHHHHCCCCHHCCCCCEEEECCCCCCEEEEEECCCCCCEEEEE
DGQIGYVGGFNIGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISY
ECCEEEEECCCCCHHHCCCCCCCCCEECCEEEEEECCCCEEEEEEEEECCCCCCCCCCCC
DDRYFPDVNSDETIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHC
SIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDD
CEEEEEECCEEEEEEECCCCCCCEEEEEEHHHHHHHHHCCEEEEEECCCEEECEEEEEEC
EIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
HHHHCCCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
KFKEGISQLLSPIL
EHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA