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The map label for this gene is murF [H]

Identifier: 82751684

GI number: 82751684

Start: 2102137

End: 2103495

Strand: Reverse

Name: murF [H]

Synonym: SAB1966c

Alternate gene names: 82751684

Gene position: 2103495-2102137 (Counterclockwise)

Preceding gene: 82751685

Following gene: 82751683

Centisome position: 76.7

GC content: 32.97

Gene sequence:

>1359_bases
ATGATTAATGTTACATTAAAGCAAATTCAATCATGGATTCCTTGCAAAATTGAAGATCAATTTTTAAATCAAGAGATAAA
TGGAGTCACAATTGATTCACGAGCAATTTCTAAAAATATGTTATTTATACCATTTAAAGGTGAAAATGTTGACGGTCATC
GCTTTGTCTCTAAAGCATTACAAGATGGTGCTGGAGCTGCTTTTTATCAAAGAGGGACACCTATAGATGAAAATGTAAGC
GGGCCTATTATATGGGTTGAAGACACATTAACGGCATTACAACAATTGGCACAAGCTTACTTGAGACATGTAAACCCTAA
AGTAATTGCCGTCACAGGGTCTAATGGTAAAACAACGACTAAAGATATGATTGAAAGTGTATTGCATACCGAATTTAAAG
TTAAGAAAACGCAAGGTAATTACAATAATGAAATTGGTTTACCTTTAACTATTTTGGAATTAGATAATGATACTGAAATA
TCAATATTGGAGATGGGGATGTCAGGTTTCCATGAAATTGAATTTCTGTCAAACCTCGCTCAACCAGATATTGCAGTTAT
AACTAATATTGGTGAGTCACATATGCAAGATTTAGGTTCTCGCGAGGGGATTGCTAAAGCTAAATCTGAAATTACAATAG
GTTTAAAAGATAATGGTACGTTTATATATGATGGCGATGAACCATTATTGAAACCGCATGTTAAAGAAGTTGAAAATGCA
AAATGTATTAGTATTGGTGTTGCTACTGATAATGCATTAGTTTGTTCTGTTGATGATAGAGATACTACAGGTATTTCATT
TACGATTAATAATAAAGAACATTATGATCTGCCAATATTAGGAAAGCATAATATGAAAAATGCGACGATTGCCATTGCGG
TTGGTCATGAATTAGGTTTGACATATAACAGAATCTATCAAAATTTAAAAAATGTCAGCTTAACTGGTATGCGTATGGAG
CAACATACATTAGAAAATGATATTACTGTGATAAATGATGCCTATAATGCAAGTCCTACAAGTATGAGAGCAGCTATTGA
TACACTGAATACTTTGACAGGGCGTCGCATTCTAATTTTAGGAGATGTTTTAGAATTAGGTGAAAATAGCAAAGAAATGC
ATATCGGTGTAGGTAATTATTTAGAAGAAAAGCATATAGATGTGTTGTATACGTTTGGTAATGAAGCGAAGTATATTTAT
GATTCGGGCCAGCAACATGTCGAAAAAGCACAACACTTCAATTCTAAAGACGATATGATAGAAGTTTTAACAAGTGATTT
AAAAGCGCATGATCGTGTATTAGTTAAAGGATCACGTGGTATGAAGTTAGAAGAAGTCGTAAATGCTTTAATTTCATAG

Upstream 100 bases:

>100_bases
CTTATCCAGAATTGATTACAAAACTTATCGAGCTTGCTAAAGAACGTCACCAGGATAAACAGAAAAATAAATACAAAATT
GACTAACTGAGGTTGTTATT

Downstream 100 bases:

>100_bases
AGATTAGTCGAGCGACCTTTTACTTATAAAAATGATTTGAATTAATACTAAAAGATTACAAAGAAGAGGTGGTTTTGTGT
GTAAATACAAAATTGCCTTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MINVTLKQIQSWIPCKIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVS
GPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNRIYQNLKNVSLTGMRME
QHTLENDITVINDAYNASPTSMRAAIDTLNTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIY
DSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS

Sequences:

>Translated_452_residues
MINVTLKQIQSWIPCKIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVS
GPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNRIYQNLKNVSLTGMRME
QHTLENDITVINDAYNASPTSMRAAIDTLNTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIY
DSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS
>Mature_452_residues
MINVTLKQIQSWIPCKIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVS
GPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNRIYQNLKNVSLTGMRME
QHTLENDITVINDAYNASPTSMRAAIDTLNTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIY
DSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=456, Percent_Identity=31.7982456140351, Blast_Score=201, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50096; Mature: 50096

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVTLKQIQSWIPCKIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKAL
CCEEEHHHHHHCCCCEEEHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
QDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTT
HCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
KDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEISILEMGMSGFHEIEFLSNLA
HHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCC
QPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
CCCEEEEECCCHHHHHHCCCCCCHHHCCCEEEEEEECCCEEEECCCCCCCCCHHHHCCCC
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGL
EEEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCEECCCCCCCEEEEEEECCCCCC
TYNRIYQNLKNVSLTGMRMEQHTLENDITVINDAYNASPTSMRAAIDTLNTLTGRRILIL
CHHHHHHHHHCCEEEEEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEE
GDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMI
ECHHHHCCCCCEEEEECCCHHCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCHHHHH
EVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MINVTLKQIQSWIPCKIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKAL
CCEEEHHHHHHCCCCEEEHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
QDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTT
HCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
KDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEISILEMGMSGFHEIEFLSNLA
HHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCC
QPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
CCCEEEEECCCHHHHHHCCCCCCHHHCCCEEEEEEECCCEEEECCCCCCCCCHHHHCCCC
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGL
EEEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCEECCCCCCCEEEEEEECCCCCC
TYNRIYQNLKNVSLTGMRMEQHTLENDITVINDAYNASPTSMRAAIDTLNTLTGRRILIL
CHHHHHHHHHCCEEEEEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEE
GDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMI
ECHHHHCCCCCEEEEECCCHHCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCHHHHH
EVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]