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The map label for this gene is leuD

Identifier: 82751663

GI number: 82751663

Start: 2072724

End: 2073296

Strand: Direct

Name: leuD

Synonym: SAB1945

Alternate gene names: 82751663

Gene position: 2072724-2073296 (Clockwise)

Preceding gene: 82751662

Following gene: 82751664

Centisome position: 75.58

GC content: 33.16

Gene sequence:

>573_bases
ATGGCAGCAATCAAACCTATTAAAACATATAAAGGTAAAATAGTCCCTCTTTTCAACGACAATATCGATACAGACCAGAT
TATTCCTAAGGTACACTTAAAGCGTATTTCAAAAAGTGGCTTTGGTCCATTTGCTTTTGATGAATGGCGGTACTTACCTG
ATGGTTCAGATAATCCTGATTTCAATCCAAATAAGCCTCAATATAAAGGTGCATCCATATTAATTACTGGAGATAATTTC
GGATGTGGTTCAAGTCGTGAACATGCAGCTTGGGCACTTAAGGACTATGGCTTTCATATTATTATTGCAGGAAGTTTCAG
TGACATTTTTTATATGAATTGTACAAAAAATGCAATGTTACCAATTGTTTTAGAAAAAAATGCACGTGAACATCTTGCGA
AATATGTTGAAATTGAGGTCGATTTACCAAATCAAACTGTGTCATCACCAGACAAGAGTTTCCATTTTGAAATTGATGAA
ACTTGGAAGAATAAACTTGTAAATGGCTTAGATGACATTGCAATCACCCTACAATATGAATCATTAATAGAAAAATATGA
AAAATCACTTTAA

Upstream 100 bases:

>100_bases
ACAAGGCAAAGGTGCTAGAACACATTTAGTGTCCCCTGCTATGGCAGCAGCCGCAGCTATTCATGGTAAATTTGTGGACG
TAAGAAAGGTGGTTGTTTAA

Downstream 100 bases:

>100_bases
GGGAGTTGAATATTATGACAGTCAAAACAACAGTTTCTACGAAAGATATCGATGAAGCATTTTTAAGACTTAAAGATATT
GTCAAAGAAACACCTTTACA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAAIKPIKTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNF
GCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
TWKNKLVNGLDDIAITLQYESLIEKYEKSL

Sequences:

>Translated_190_residues
MAAIKPIKTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNF
GCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
TWKNKLVNGLDDIAITLQYESLIEKYEKSL
>Mature_189_residues
AAIKPIKTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFG
CGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDET
WKNKLVNGLDDIAITLQYESLIEKYEKSL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=186, Percent_Identity=46.2365591397849, Blast_Score=172, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6321429, Length=200, Percent_Identity=48, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21564; Mature: 21433

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIKPIKTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPD
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
FNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAML
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEECCCCCEE
PIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDETWKNKLVNGLDDIAITLQYE
EEEECCCHHHHHHEEEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEEHH
SLIEKYEKSL
HHHHHHHHCC
>Mature Secondary Structure 
AAIKPIKTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPD
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
FNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAML
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEECCCCCEE
PIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDETWKNKLVNGLDDIAITLQYE
EEEECCCHHHHHHEEEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEEHH
SLIEKYEKSL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA