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The map label for this gene is ilvB

Identifier: 82751657

GI number: 82751657

Start: 2065567

End: 2067336

Strand: Direct

Name: ilvB

Synonym: SAB1939

Alternate gene names: 82751657

Gene position: 2065567-2067336 (Clockwise)

Preceding gene: 82751656

Following gene: 82751658

Centisome position: 75.32

GC content: 36.67

Gene sequence:

>1770_bases
ATGTCTAAAACTCAACATGAAGTAAACCAAAATATTGACCCTTTAAAAATGGCTGAATCACTTGAACCTGAACAACTAAA
TGAAAAAACTTTAAATGACATGCGTTCAGGATCAGAAGTGCTAGTAGAAGTTCTACTTAAAGAAAATGTGGATTATTTAT
TCGGTTATCCTGGTGGTGCCGTACTACCTTTATACGACACATTTTATGATGGTAAAATCAAACATATTTTAGCAAGACAC
GAACAAGGTGCTGTTCATGCTGCAGAAGGTTATGCACGTGTATCTGGTAAAACTGGCGTCGTTGTAGTTACAAGCGGTCC
AGGTGCAACTAATGTAATGACAGGTATTACGGATGCACATTGCGACTCTTTACCTCTAGTTGTATTCACTGGACAAGTTG
CTACACCAGGCATTGGTAAAGATGCATTCCAAGAAGCGGATATTCTATCTATGACTTCACCAATTACAAAACAAAATTAT
CAAGTGAAACGTGTTGAAGATATCCCTAAAATCGTACACGAAGCTTTCCATGTAGCTAATTCTGGACGCAAAGGTCCTGT
AGTGATTGATTTTCCAAAAGATATGGGTGTTTTAGCTACAAATGTGGATTTATGCGACGAAATCAATATTCCAGGTTATG
AAGTTGTTACAGAACCAGAAAATAAAGACATTGAGACTTTCATCTCACTTTTAAAAGAAGCGAAAAAGCCTGTCGTATTA
GCCGGCGCAGGTATTAATCAATCAAAATCAAATCAATTATTAACACAGTTTGTTAATAAACATCAAATCCCAACAGTTAC
TACTTTACTAGGTCTAGGTGCTGTACCTTATGAGGATACACTATTTTTAGGTATGGGAGGTATGCATGGTTCTTATGCTA
GTAACATGGCATTAACTGAGTGTGATTTACTCATTAATTTAGGTAGCCGCTTCGATGATAGATTAGCAAGCAAACCTGAT
GCCTTTGCACCTAACGCCAAAATTGTACATGTAGATATTGACCCTTCAGAAATTAATAAAGTTATTCATGTAGATTTAGG
TATTATTGCAGACTGTAAACGATTTTTAGAATGTTTAAATGATAAAAATGTTGAGACTATAGAACACAGTGACTGGGTTA
ACCATTGTCAAAATAATAAGCAGAAACACCCGTTTAAACTTGGTGAAGAAGATCCAGTATTTTGTAAGCCACAACAAACA
ATCGAATATATCGGCAAAATTACAAATGGTGAAGCAATTGTTACTACAGACGTGGGACAACATCAAATGTGGGCAGCTCA
ATTTTATCCATTTAAAAATCACGGACAATGGGTTACAAGCGGTGGTTTAGGAACAATGGGATTCGGTATTCCTTCGTCAA
TTGGTGCCAAATTAGCTAATCCTGATAAAACAGTCGTATGTTTCGTCGGTGACGGTGGTTTCCAAATGACAAACCAAGAA
ATGGCACTATTACCCGAATATGGTTTAGATGTCAAAATCGTACTAATCAATAATGGAACATTAGGTATGGTTAAACAATG
GCAAGATAAGTTCTTTAATCAACGCTTCTCACACTCAGTATTTAATGGTCAACCTGATTTTATGAAAATGGCAGAAGCAT
ATGGCGTCAAAGGTTTCTTAATCGATAAGCCAGAACAACTGGAAGAACAATTAGATGTAGCGTTTGCTTATCAAGGACCA
GCTTTAATTGAGGTTCGTATTTCCCCTACTGAAGCTGTAAACCCAATGGTTCCGAGTGGTAAATCAAATCATGAAATGGA
GGGCTTATAA

Upstream 100 bases:

>100_bases
AGCTCGTTATACTGCCCTAGTAACTAGCGCAAATACAGGTGGCGTCATGCAAGTGCCTGAGAATTTAATTTAATTTATTT
TTATATTGGAGATGGTTAAA

Downstream 100 bases:

>100_bases
TGACAAGAATTCTTAAATTACAAGTTGCGGATCAAGTTAGCACGCTAAATCGAATTACAAGTGCTTTTGTTCGCCTACAA
TATAATATCGATACATTACA

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEVLLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARH
EQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIETFISLLKEAKKPVVL
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPD
AFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVNHCQNNKQKHPFKLGEEDPVFCKPQQT
IEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDVAFAYQGP
ALIEVRISPTEAVNPMVPSGKSNHEMEGL

Sequences:

>Translated_589_residues
MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEVLLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARH
EQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIETFISLLKEAKKPVVL
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPD
AFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVNHCQNNKQKHPFKLGEEDPVFCKPQQT
IEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDVAFAYQGP
ALIEVRISPTEAVNPMVPSGKSNHEMEGL
>Mature_588_residues
SKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEVLLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHE
QGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQ
VKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIETFISLLKEAKKPVVLA
GAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDA
FAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVNHCQNNKQKHPFKLGEEDPVFCKPQQTI
EYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQEM
ALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDVAFAYQGPA
LIEVRISPTEAVNPMVPSGKSNHEMEGL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=24.0143369175627, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=24.5960502692998, Blast_Score=144, Evalue=3e-34,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=42.5795053003534, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=41.9014084507042, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=544, Percent_Identity=32.7205882352941, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=26.4705882352941, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=23.8779174147217, Blast_Score=132, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=564, Percent_Identity=25, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=564, Percent_Identity=25, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=604, Percent_Identity=25.8278145695364, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=600, Percent_Identity=41.3333333333333, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=24.223602484472, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=487, Percent_Identity=22.17659137577, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320588, Length=618, Percent_Identity=19.7411003236246, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=566, Percent_Identity=27.5618374558304, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64530; Mature: 64399

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEVLLKENVDYLFGYPGGA
CCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCC
VLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAH
EEEEHHHHCCCHHHHHHHHCCCCCEECCCCEEEECCCCCEEEEECCCCCCHHHCCCCCCC
CDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQVKRVEDIPKIVHEAFHVAN
CCCCCEEEEECCCCCCCCCHHHHHCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
SGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIETFISLLKEAKKPVVL
CCCCCCEEEECCCCCCEEEECCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTE
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCCHHH
CDLLINLGSRFDDRLASKPDAFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLN
HHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCHHHCCEEEEECCCHHHHHHHHHHHHC
DKNVETIEHSDWVNHCQNNKQKHPFKLGEEDPVFCKPQQTIEYIGKITNGEAIVTTDVGQ
CCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCEEEEECCCC
HQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
CCEEHHHCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCEECCCC
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFL
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCEEE
IDKPEQLEEQLDVAFAYQGPALIEVRISPTEAVNPMVPSGKSNHEMEGL
ECCHHHHHHHHCEEEEECCCEEEEEEECCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEVLLKENVDYLFGYPGGA
CCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCC
VLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAH
EEEEHHHHCCCHHHHHHHHCCCCCEECCCCEEEECCCCCEEEEECCCCCCHHHCCCCCCC
CDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQVKRVEDIPKIVHEAFHVAN
CCCCCEEEEECCCCCCCCCHHHHHCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
SGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIETFISLLKEAKKPVVL
CCCCCCEEEECCCCCCEEEECCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTE
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCCHHH
CDLLINLGSRFDDRLASKPDAFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLN
HHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCHHHCCEEEEECCCHHHHHHHHHHHHC
DKNVETIEHSDWVNHCQNNKQKHPFKLGEEDPVFCKPQQTIEYIGKITNGEAIVTTDVGQ
CCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCEEEEECCCC
HQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
CCEEHHHCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCEECCCC
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFL
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCEEE
IDKPEQLEEQLDVAFAYQGPALIEVRISPTEAVNPMVPSGKSNHEMEGL
ECCHHHHHHHHCEEEEECCCEEEEEEECCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]