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The map label for this gene is nrgA [H]

Identifier: 82751645

GI number: 82751645

Start: 2052436

End: 2053686

Strand: Reverse

Name: nrgA [H]

Synonym: SAB1927c

Alternate gene names: 82751645

Gene position: 2053686-2052436 (Counterclockwise)

Preceding gene: 82751646

Following gene: 82751644

Centisome position: 74.88

GC content: 33.01

Gene sequence:

>1251_bases
ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGCTAATGACACCAGGATTAAGTTTATTTTA
TGGTGGGTTAGTTCAATCTAAAAATGCGCTAAATACTGTCATGCAAAGTATGCCCGCAATTGTGCTTGTTACATTTGTAT
GGATAACAGTTGGTTTTACAATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTATTTCAAATGATGTTTTGTAC
GATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAAATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGA
CTGCTCTTGTATACAGTCCAGTAGCACATTGGGTTTGGGGCGACGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
GGAGGTACAGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATTGGAAAAGGAAACAAACATTC
TGAATCAACACCACATAATCTTATCATTACGTTGATTGGCGGTATATTTGTGTGGATTGGTTGGTATGGATTTAATGTAG
GTAGTGCTTTTACATTTGATAATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
TGGTTAATTCTAGAATATATTTTTAAAAAGACTACAAGTTTACTAGGATTATTATTAGGTACATTAGCTGGTTTAGTTGT
CATTACACCTGTAGCAGGATACGTGACATATCTTAGTGCAACAATCATGGCAATGATCGGCGGTATCTGTTGCTATATCG
TTATTAATTACATCAAGGTAAAACTAAAATATCATGATACATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
GGTGCAGTATTAACAGCCTTTTTCCAAAGTAAAAAGGCAAATCCTGACATTGAGAATGGCTTTATTTATACTGGTGACAT
ACATATTATACTTGTACAAATATTATGTGTAACAGCAGTTGTAATTTTTAGTATCGTCATGACGTTTATTATTGCGAAAG
TAATTAAATTAATTACACCATTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG

Upstream 100 bases:

>100_bases
ATAGCGTGATACATTTTATTCCAAAAGATGTAATAAAACTTTACGTATTTTTGCTTTTTATAAATTGTCGGATTATTATG
AAAACAAGGGAGTGGTAAGT

Downstream 100 bases:

>100_bases
AATATATACATAGAATATTCATTGTCCTGACATTTAACTAAAGGTTGATGTTGGGACATTTTGTTATACAAAAGTTTTAT
TTTGAAATCTTTTTATGAAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMPAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGDGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGTLAGLVVITPVAGYVTYLSATIMAMIGGICCYIVINYIKVKLKYHDTLDAFGIHGVGGII
GAVLTAFFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY

Sequences:

>Translated_416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMPAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGDGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGTLAGLVVITPVAGYVTYLSATIMAMIGGICCYIVINYIKVKLKYHDTLDAFGIHGVGGII
GAVLTAFFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY
>Mature_416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMPAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGDGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGTLAGLVVITPVAGYVTYLSATIMAMIGGICCYIVINYIKVKLKYHDTLDAFGIHGVGGII
GAVLTAFFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=403, Percent_Identity=39.2059553349876, Blast_Score=272, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI133931100, Length=427, Percent_Identity=25.0585480093677, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=416, Percent_Identity=26.2019230769231, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=27.6255707762557, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=431, Percent_Identity=25.754060324826, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=412, Percent_Identity=33.495145631068, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=32.4519230769231, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=31.9711538461538, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=29.0865384615385, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45718; Mature: 45718

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMPAIVLVTFVWITVGFT
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEK
EEECCCEEEECCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFIPYLLFVVIWTALVYSPVAHWVWGDGWINKLGVLDFAGGTVVHITSGVSGLVLAIMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCEEEEEECCHHHHHHEEEE
GKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHH
WLILEYIFKKTTSLLGLLLGTLAGLVVITPVAGYVTYLSATIMAMIGGICCYIVINYIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KLKYHDTLDAFGIHGVGGIIGAVLTAFFQSKKANPDIENGFIYTGDIHIILVQILCVTAV
EEEECCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
VIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMPAIVLVTFVWITVGFT
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEK
EEECCCEEEECCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFIPYLLFVVIWTALVYSPVAHWVWGDGWINKLGVLDFAGGTVVHITSGVSGLVLAIMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCEEEEEECCHHHHHHEEEE
GKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHH
WLILEYIFKKTTSLLGLLLGTLAGLVVITPVAGYVTYLSATIMAMIGGICCYIVINYIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KLKYHDTLDAFGIHGVGGIIGAVLTAFFQSKKANPDIENGFIYTGDIHIILVQILCVTAV
EEEECCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
VIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]