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The map label for this gene is pckA

Identifier: 82751373

GI number: 82751373

Start: 1790547

End: 1792139

Strand: Direct

Name: pckA

Synonym: SAB1646

Alternate gene names: 82751373

Gene position: 1790547-1792139 (Clockwise)

Preceding gene: 82751369

Following gene: 82751376

Centisome position: 65.29

GC content: 34.59

Gene sequence:

>1593_bases
ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACGTCACATTTTCAACTTTCGAC
GACACAACTTTATAATAAAATCTTAGATAATAACGAAGGGGTATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAA
AATATACTGGTCGTTCGCCTAAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTAGATAAAAAAGATGAATTATA
CGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATGTTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATT
TATTTGCTAAAAATATGTTTATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
GTCCCACATTTTAAAGCAGATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATTATTTCATTTAAACACAAAGT
CATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAAAAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGC
AAGATATTATGAGCATGCATTGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
GGTAAAACAACCCTATCAGCAGATCCACACCGTAAACTAATCGGTGATGATGAACACGGCTGGAATAAAAACGGGGTCTT
TAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTTTCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAAT
ATGGTGCAATTTTAGAGAACACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGATAATCGTTATACAGAAAATACA
CGTGCGGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCAAAAGCAGCACATCCAAATACAATTATTTTCTT
AACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAGTTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTT
TCACTTCTAAATTAGCTGGTACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGCGCACCGTTC
TTCCCGTTACACCCTACTGTTTACGCTGATTTATTAGGTGAACTTATCGATTTACATGATGTTGATGTTTATCTTGTTAA
TACTGGATGGACTGGCGGAAAATATGGTGTAGGACGTAGAATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGA
TTTCTGGCAAATTGAAAAATGCAGAATATACAAAAGATAGTACGTTTGGTTTAAGTATTCCTGTAGAAATTGAAGATGTA
CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAAGCAGAAGATTTAATTCAACG
TTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAACATATTGCTAAAAAAGGTAGCTTCAACAAATAA

Upstream 100 bases:

>100_bases
ATAAATTTACAGTAAAATATTGTAGATTAATTATGTTAATGTGTTATACTAATTAAATGTAAAGGCTTACATTTAAATTA
TCGCTTTGGAGGGATTTAGG

Downstream 100 bases:

>100_bases
ATTTGAATCCTAATTAAAGAGCCAACCGTTGTTATAGTCTAACATTGGTTGGCTCCTCTTATTTTATATGGTAAGAGTTT
ATAGGCAATTTTATTACAAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEI
NQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
VPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENT
RAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPF
FPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAKKGSFNK

Sequences:

>Translated_530_residues
MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEI
NQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
VPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENT
RAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPF
FPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAKKGSFNK
>Mature_529_residues
SVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEIN
QPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSV
PHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTG
KTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTR
AAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFF
PLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVP
KTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAKKGSFNK

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=503, Percent_Identity=52.4850894632207, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322950, Length=496, Percent_Identity=49.1935483870968, Blast_Score=491, Evalue=1e-139,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59405; Mature: 59274

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSP
CCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
KDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTM
CCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCE
LKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSVPHFKADPEVDGTKSETFVI
EEEEHHHHHHHHHHHHHCEEECCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
ISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
EEECCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILEN
CCEEECCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
TVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISK
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCEEEEEECCCCCCCCCHHH
LNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
VDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
CEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHH
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAKKGSFNK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSP
CCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
KDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTM
CCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCE
LKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSVPHFKADPEVDGTKSETFVI
EEEEHHHHHHHHHHHHHCEEECCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
ISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGT
EEECCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
GKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILEN
CCEEECCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
TVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISK
EEEECCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCEEEEEECCCCCCCCCHHH
LNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHD
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
VDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDV
CEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHH
PKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAKKGSFNK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA