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The map label for this gene is ribA

Identifier: 82751354

GI number: 82751354

Start: 1772248

End: 1773429

Strand: Reverse

Name: ribA

Synonym: SAB1626c

Alternate gene names: 82751354

Gene position: 1773429-1772248 (Counterclockwise)

Preceding gene: 82751355

Following gene: 82751353

Centisome position: 64.66

GC content: 34.35

Gene sequence:

>1182_bases
ATGCAATTCGATAATATTGACAGTGCTTTAATGGCTTTAAAAAATGGAGAACCAATTATTGTAGTAGATGATGAGAATCG
TGAAAATGAAGGTGATTTAGTAGCGGTCACTGAATGGATGAACGATAATACCATTAATTTTATGGCGAAAGAAGCAAGGG
GATTAATATGCGCACCAGTGTCTAAAGATATTGCACAACGTTTGGATTTGGTACAAATGGTTGATGATAACTCCGACATC
TTTGGTACGCAATTTACAGTGAGTATTGATCATGTAGATACAACAACAGGAATTAGCGCTTATGAACGTACATTGACTGC
CAAAAAGCTCATTGATCCTAGTAGTGAAGCTAAAGATTTTAATCGTCCTGGTCATTTATTTCCATTAGTAGCACAAGATA
AAGGCGTATTAGCTAGAAATGGACACACAGAAGTGGCTGTTGATTTAGCTAAACTTACTGGTGCCAAGCCCGCTGGTGTC
ATTTGTGAGATTATGAATGATGACGGCACGATGGCGAAAGGACAAGATTTACAAAAGTTTAAAGAAAAACATCAATTAAA
GATGATTACGATTGATGATTTAATTGAATATCGTAAAAAATTAGAACCAGAAATTGAATTTAAGGCAAAAGTGAAAATGC
CTACAGATTTCGGAACATTTGATATGTATGGTTTTAAAGCGACTTACACAGATGAAGAGATAGTTGTACTGACAAAAGGT
GCAATTCGACAACATGAAAATGTACGCTTACATTCTGCGTGCCTTACAGGCGATATTTTCCATAGTCAACGTTGTGATTG
TGGTGCTCAACTTGAATCGTCTATGAAGTATATCAATGAACATGGCGGCATGATTATTTATCTACCTCAAGAAGGTCGTG
GCATAGGATTGTTAAACAAATTACGCGCATATGAATTAATTGAGCAAGGATATGATACAGTAACTGCAAATTTAGCATTA
GGTTTTGATGAAGATTTGCGAGATTATCATATTGCTGCACAGATTTTAAAATATTTTAACATCGAACATATCAATTTATT
AAGTAATAATCCAAGTAAATTTGAGGGATTAAAACAATATGGCATTGATATTGCAGAAAGAATTGAAGTTATCGTACCAG
AAACGGCACATAATCATGATTATATGGAAACGAAAAAAATAAAAATGGGTCATTTAATATAG

Upstream 100 bases:

>100_bases
ACAGACGTTTTGTTTAAATATGTTGAAAATATTTTAAATAAAGATAAAGACCAATTATCTGTAGATAAATTAAGAGCATT
TGGTTTTTAGGAGGGGTAGC

Downstream 100 bases:

>100_bases
GAGGACTTTAATATGAATTTTGAAGGTAAATTAATTGGAAAAGATTTGAAAGTTGCAATCGTAGTTAGTCGATTTAATGA
TTTTATCACTGGAAGATTAC

Product: riboflavin biosynthesis protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDI
FGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEVAVDLAKLTGAKPAGV
ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLAL
GFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETAHNHDYMETKKIKMGHLI

Sequences:

>Translated_393_residues
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDI
FGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEVAVDLAKLTGAKPAGV
ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLAL
GFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETAHNHDYMETKKIKMGHLI
>Mature_393_residues
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDI
FGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEVAVDLAKLTGAKPAGV
ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLAL
GFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETAHNHDYMETKKIKMGHLI

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=43.7810945273632, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787533, Length=188, Percent_Identity=48.936170212766, Blast_Score=176, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=42.9951690821256, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319438, Length=169, Percent_Identity=37.8698224852071, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 44189; Mature: 44189

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPV
CCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEHHCCCEEEECC
SKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDF
CHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
NRPGHLFPLVAQDKGVLARNGHTEVAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKF
CCCCCEEEEEECCCCEEEECCCCCEEEEHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
KEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
HHHHCEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCEEEECCCCEEEEEECC
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNK
CCCCCCCCEEEHHEEECCHHCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
LRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQY
HHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
GIDIAERIEVIVPETAHNHDYMETKKIKMGHLI
CCCHHHCEEEEECCCCCCCCCHHHHCEECCCCC
>Mature Secondary Structure
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPV
CCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEHHCCCEEEECC
SKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDF
CHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
NRPGHLFPLVAQDKGVLARNGHTEVAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKF
CCCCCEEEEEECCCCEEEECCCCCEEEEHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
KEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
HHHHCEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCEEEECCCCEEEEEECC
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNK
CCCCCCCCEEEHHEEECCHHCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
LRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQY
HHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
GIDIAERIEVIVPETAHNHDYMETKKIKMGHLI
CCCHHHCEEEEECCCCCCCCCHHHHCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA