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The map label for this gene is iscS2 [H]

Identifier: 82751304

GI number: 82751304

Start: 1704773

End: 1705921

Strand: Reverse

Name: iscS2 [H]

Synonym: SAB1575c

Alternate gene names: 82751304

Gene position: 1705921-1704773 (Counterclockwise)

Preceding gene: 82751305

Following gene: 82751303

Centisome position: 62.2

GC content: 31.94

Gene sequence:

>1149_bases
GTGTTTATTTTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTAGATACTTATTTAAAAGTAAA
TCAATCAATGTATTATAATCCGAATAGTCCGCATAAAGCTGGTTTGCAGGCAAATCAATTACTACAACAAGCAAAAGCTC
AAATTAATGCAATGATTAATTCAAAAACAAATTATGATGTTGTATTCACTAGTGGTGCAACGGAATCTAACAATATCGCG
CTTAAAGGTGTGGCTTATCGTAAATTTGATACAGCTAAGGAAATAATTACATCCGTGTTAGAACATCCATCCGTATTAGA
AGTTGTCAGATATTTGGAAGCGCACGAAGGATTTAAAGTTAAATATGTTGATGTAAAGAAAGATGGCAGTATTAACTTAG
AACACTTCAAAGAATTAATGTCAGACAAAGTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACAGGACAAATACAG
CCTATTCCACAAATGGCTGAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGATGCGGTTCAAGCATTCGGCAA
AATTTCAATGGATTTAACAAATGTAGATAGTATTAGTTTAAGTGGACACAAGTTTAATGGTTTAAAAGGTCAAGGCGTCT
TACTTGTAAATCACATTCAAAATGTTGAACCAACTGTCCATGGTGGTGGTCAAGAATATGGCGTTAGAAGTGGAACAGTT
AATTTGCCAAATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACCTTGAAGCATTGAATGCATTTGTTAC
TGAGTTAAATAATGACGTCCGTCAATTTTTAAATAAATATCAAGGTGTTTATATTAATTCATCCGCTTCAGGTTCACCAT
TCGTTTTAAATATTAGTTTTCCTGGCGTAAAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCT
ACGACGAGTGCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGATTATCAGACAAATCTATTGA
AGGTAGTATAAGATTATCATTTGGGGCTACAACAACTAAAGAAGATATAGCAAGGTTTAAAGAAATATTTATCATCATTT
ATGAGGAAATTAAGGAGTTGCTAAAATAA

Upstream 100 bases:

>100_bases
GAAATAAGATTATTTTGCGCTATAACCTTTATTTTTTGTCGGATAATAGTATAATGCTTGAGTAAAACAATTTTAACGAT
TGATGATTAATGAAAGAGAA

Downstream 100 bases:

>100_bases
TGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGAAAGAAATTTGTAAATCAATTA
AGAAATAATGTAAATAAGTC

Product: aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNIA
LKGVAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDLTNVDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTV
NLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQFLNKYQGVYINSSASGSPFVLNISFPGVKGEVLVNAFSKYDIMIS
TTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

Sequences:

>Translated_382_residues
MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNIA
LKGVAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDLTNVDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTV
NLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQFLNKYQGVYINSSASGSPFVLNISFPGVKGEVLVNAFSKYDIMIS
TTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK
>Mature_382_residues
MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNIA
LKGVAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDLTNVDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTV
NLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQFLNKYQGVYINSSASGSPFVLNISFPGVKGEVLVNAFSKYDIMIS
TTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=35.509138381201, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI156713448, Length=404, Percent_Identity=29.2079207920792, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI48994898, Length=371, Percent_Identity=38.0053908355795, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1787970, Length=231, Percent_Identity=26.4069264069264, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789175, Length=385, Percent_Identity=23.3766233766234, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=36.9791666666667, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17533177, Length=312, Percent_Identity=29.1666666666667, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319831, Length=365, Percent_Identity=37.8082191780822, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=35.9375, Blast_Score=208, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42223; Mature: 42223

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMIN
CEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCC
SKTNYDVVFTSGATESNNIALKGVAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKV
CCCCEEEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEE
KYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQPIPQMAEVIKNYPKAHFHVD
EEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEECCHHHHHHHHHCCCCEEEHH
AVQAFGKISMDLTNVDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTV
HHHHHCCCEEEEECCCEEEECCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
NLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQFLNKYQGVYINSSASGSPFVLNISF
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEC
PGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTK
CCCCCHHHEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCH
EDIARFKEIFIIIYEEIKELLK
HHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMIN
CEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCC
SKTNYDVVFTSGATESNNIALKGVAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKV
CCCCEEEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEE
KYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQPIPQMAEVIKNYPKAHFHVD
EEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEECCHHHHHHHHHCCCCEEEHH
AVQAFGKISMDLTNVDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTV
HHHHHCCCEEEEECCCEEEECCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
NLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQFLNKYQGVYINSSASGSPFVLNISF
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEC
PGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTK
CCCCCHHHEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCH
EDIARFKEIFIIIYEEIKELLK
HHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]